ID   IPT_RALSL               Reviewed;         238 AA.
AC   P14333;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
DE   AltName: Full=Trans-zeatin producing protein;
GN   Name=tzs;
OS   Ralstonia solanacearum (Pseudomonas solanacearum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K60;
RX   PubMed=2587241; DOI=10.1093/nar/17.21.8886;
RA   Akiyoshi D.E., Regier D.A., Gordon M.P.;
RT   "Nucleotide sequence of the tzs gene from Pseudomonas solanacearum strain
RT   K60.";
RL   Nucleic Acids Res. 17:8886-8886(1989).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X16381; CAA34418.1; -; Genomic_DNA.
DR   PIR; S06739; S06739.
DR   AlphaFoldDB; P14333; -.
DR   SMR; P14333; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Cytokinin biosynthesis; Transferase.
FT   CHAIN           1..238
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216437"
SQ   SEQUENCE   238 AA;  26440 MW;  D250E48455CBA8D9 CRC64;
     MPIRLYLIWG ATTTGKTAQS VALARATGAP VISLDRVQCC HELAVGSGRP LPSELLGTRR
     EYLCDRSVSS GVIPAADANQ LLFDKVVRYS AHERALILEG GSVSLINAMI RDARWSALGE
     WALRRIVLPG RAVFIAQAIK RVRDMLEPPP GHASILDELE GLWAHPGNHA VLEDVDGYRQ
     IIRYARASQV PIDRITSIDR STMATLVERI AQEYWEHALW QEQEFLGIPA TWRRADDA