ID   IPT_RHIRD               Reviewed;         239 AA.
AC   P15653;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
GN   Name=ipt;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiBo542.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bo542;
RX   PubMed=2747621; DOI=10.1007/bf00334380;
RA   Strabala T.J., Bednarek S.Y., Bertoni G., Amasino R.;
RT   "Isolation and characterization of an ipt gene from the Ti plasmid Bo542.";
RL   Mol. Gen. Genet. 216:388-394(1989).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X14410; CAA32582.1; -; Genomic_DNA.
DR   PIR; S04421; JJAGBT.
DR   AlphaFoldDB; P15653; -.
DR   SMR; P15653; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..239
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216430"
SQ   SEQUENCE   239 AA;  26873 MW;  E11150D857CE4663 CRC64;
     MDLRLIFGPT CTGKTSTAIA LAQQTGLPVL SLDRVQCCPQ LSTGSGRPTV EELKGTTRLY
     LDDRPLVKGI ITAKQAHERL IAEVHNHEAK GGLILEGGSI SLLRCMAQSR YWNADFRWHI
     IRNELADEES FMSVAKTRVK QMLRPSAGLS IIQELVQLWR EPRLRPILEG IDGYRYALLF
     ATQNQITPDM LLQLDADMEN KLIHGIAQEF LIHARRQEQK FPLVGATAVE AFEGPPFRM