ID   IPYR_CYAPA              Reviewed;          25 AA.
AC   P80887;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   11-DEC-2019, entry version 26.
DE   RecName: Full=Inorganic pyrophosphatase;
DE            EC=3.6.1.1;
DE   AltName: Full=Pyrophosphate phospho-hydrolase;
DE            Short=PPase;
DE   Flags: Fragment;
OS   Cyanophora paradoxa.
OC   Eukaryota; Glaucocystophyceae; Cyanophoraceae; Cyanophora.
OX   NCBI_TaxID=2762;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES,
RP   SUBUNIT, AND MASS SPECTROMETRY.
RC   STRAIN=UTEX LB 555 / Pringsheim {ECO:0000269|PubMed:16313235};
RX   PubMed=16313235; DOI=10.1042/bj20051657;
RA   Gomez-Garcia M.R., Losada M., Serrano A.;
RT   "A novel subfamily of monomeric inorganic pyrophosphatases in
RT   photosynthetic eukaryotes.";
RL   Biochem. J. 395:211-221(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474; EC=3.6.1.1;
CC         Evidence={ECO:0000269|PubMed:16313235};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q6BWA5};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=32.4 uM for Mg2-PPi {ECO:0000269|PubMed:16313235};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:16313235}.
CC   -!- MASS SPECTROMETRY: Mass=26560; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16313235};
CC   -!- SIMILARITY: Belongs to the PPase family. {ECO:0000255}.
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DR   SABIO-RK; P80887; -.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Magnesium; Metal-binding.
FT   CHAIN           1..>25
FT                   /note="Inorganic pyrophosphatase"
FT                   /id="PRO_0000253940"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:16313235"
SQ   SEQUENCE   25 AA;  2774 MW;  7F4B01D7075A02E9 CRC64;
     XAITAEPVGT PETLEYRVFI QKDGK