ID   IPYR_MAIZE              Reviewed;         214 AA.
AC   O48556;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Soluble inorganic pyrophosphatase;
DE            EC=3.6.1.1;
DE   AltName: Full=Pyrophosphate phospho-hydrolase;
DE            Short=PPase;
GN   Name=IPP;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. B73;
RA   Kadambi S.N., Baysdorfer C.;
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474; EC=3.6.1.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the PPase family. {ECO:0000305}.
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DR   EMBL; AF034947; AAB88618.1; -; mRNA.
DR   PIR; T01370; T01370.
DR   RefSeq; NP_001104889.1; NM_001111419.1.
DR   RefSeq; XP_008668607.1; XM_008670385.1.
DR   AlphaFoldDB; O48556; -.
DR   SMR; O48556; -.
DR   STRING; 4577.GRMZM2G061928_P01; -.
DR   PaxDb; O48556; -.
DR   PRIDE; O48556; -.
DR   EnsemblPlants; Zm00001eb076120_T001; Zm00001eb076120_P001; Zm00001eb076120.
DR   EnsemblPlants; Zm00001eb076120_T003; Zm00001eb076120_P003; Zm00001eb076120.
DR   EnsemblPlants; Zm00001eb076120_T004; Zm00001eb076120_P004; Zm00001eb076120.
DR   EnsemblPlants; Zm00001eb076120_T005; Zm00001eb076120_P005; Zm00001eb076120.
DR   EnsemblPlants; Zm00001eb076120_T006; Zm00001eb076120_P006; Zm00001eb076120.
DR   GeneID; 541666; -.
DR   Gramene; Zm00001eb076120_T001; Zm00001eb076120_P001; Zm00001eb076120.
DR   Gramene; Zm00001eb076120_T003; Zm00001eb076120_P003; Zm00001eb076120.
DR   Gramene; Zm00001eb076120_T004; Zm00001eb076120_P004; Zm00001eb076120.
DR   Gramene; Zm00001eb076120_T005; Zm00001eb076120_P005; Zm00001eb076120.
DR   Gramene; Zm00001eb076120_T006; Zm00001eb076120_P006; Zm00001eb076120.
DR   KEGG; zma:541666; -.
DR   eggNOG; KOG1626; Eukaryota.
DR   OrthoDB; 1429691at2759; -.
DR   Proteomes; UP000007305; Chromosome 2.
DR   ExpressionAtlas; O48556; baseline and differential.
DR   Genevisible; O48556; ZM.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR   GO; GO:0006796; P:phosphate-containing compound metabolic process; IBA:GO_Central.
DR   CDD; cd00412; pyrophosphatase; 1.
DR   Gene3D; 3.90.80.10; -; 1.
DR   HAMAP; MF_00209; Inorganic_PPase; 1.
DR   InterPro; IPR008162; Pyrophosphatase.
DR   InterPro; IPR036649; Pyrophosphatase_sf.
DR   PANTHER; PTHR10286; PTHR10286; 1.
DR   Pfam; PF00719; Pyrophosphatase; 1.
DR   SUPFAM; SSF50324; SSF50324; 1.
DR   PROSITE; PS00387; PPASE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..214
FT                   /note="Soluble inorganic pyrophosphatase"
FT                   /id="PRO_0000137576"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         64
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WI55"
FT   BINDING         78
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WI55"
FT   BINDING         90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WI55"
FT   BINDING         100
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         174
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WI55"
SQ   SEQUENCE   214 AA;  24370 MW;  F70E726561257AB9 CRC64;
     MSEEDKTAAS AEQPKRAPKL NERILSSLSR RSVAAHPWHD LEIGPDAPAV FNVVVEITKG
     SKVKYELDKK TGLIKVDRVL YSSVVYPHNY GFVPRTLCED NDPMDVLVLM QEPVVPGSFL
     RARAIGLMPM IDQGEKDDKI IAVCADDPEY RHYNDISELS PHRLQEIKRF FEDYKKNENK
     EVAVDAFLPA TTAREAIQYS MDLYAQYILQ SLRQ