IPYR_ORYSI
ID IPYR_ORYSI Reviewed; 214 AA.
AC A2X8Q3; O22537; Q6YVH9; Q9ZSU9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Soluble inorganic pyrophosphatase;
DE EC=3.6.1.1;
DE AltName: Full=Pyrophosphate phospho-hydrolase;
DE Short=PPase;
GN Name=IPP; ORFNames=OsI_008446;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Milyang 23; TISSUE=Seed;
RA Lee M.C., Kim C.S., Eun M.Y.;
RT "Isolation and characterization of inorganic pyrophosphatase from rice.";
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474; EC=3.6.1.1;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the PPase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB82136.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF022733; AAB82136.1; ALT_FRAME; mRNA.
DR EMBL; CM000127; EAY87213.1; -; Genomic_DNA.
DR PIR; T02082; T02082.
DR AlphaFoldDB; A2X8Q3; -.
DR SMR; A2X8Q3; -.
DR STRING; 39946.A2X8Q3; -.
DR EnsemblPlants; BGIOSGA005774-TA; BGIOSGA005774-PA; BGIOSGA005774.
DR Gramene; BGIOSGA005774-TA; BGIOSGA005774-PA; BGIOSGA005774.
DR HOGENOM; CLU_073198_2_1_1; -.
DR OMA; DEPTFPG; -.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblPlants.
DR GO; GO:0005654; C:nucleoplasm; IEA:EnsemblPlants.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0052546; P:cell wall pectin metabolic process; IEA:EnsemblPlants.
DR GO; GO:0052386; P:cell wall thickening; IEA:EnsemblPlants.
DR GO; GO:0006796; P:phosphate-containing compound metabolic process; IEA:InterPro.
DR CDD; cd00412; pyrophosphatase; 1.
DR Gene3D; 3.90.80.10; -; 1.
DR HAMAP; MF_00209; Inorganic_PPase; 1.
DR InterPro; IPR008162; Pyrophosphatase.
DR InterPro; IPR036649; Pyrophosphatase_sf.
DR PANTHER; PTHR10286; PTHR10286; 1.
DR Pfam; PF00719; Pyrophosphatase; 1.
DR SUPFAM; SSF50324; SSF50324; 1.
DR PROSITE; PS00387; PPASE; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..214
FT /note="Soluble inorganic pyrophosphatase"
FT /id="PRO_0000301654"
FT BINDING 64
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WI55"
FT BINDING 78
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WI55"
FT BINDING 90
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WI55"
FT BINDING 100
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 137
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WI55"
FT CONFLICT 65
FT /note="Y -> C (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="C -> R (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="L -> F (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="L -> F (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="Y -> H (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="L -> S (in Ref. 1; AAB82136)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 214 AA; 24160 MW; 9986B4D0DC5ACCB3 CRC64;
MAGEADGKAP LGSRYPPAAL NERILSSMSQ KHVAAHPWHD LEIGPGAPAV FNCVVEIPRG
SKVKYELDKA TGLIKVDRVL YSSVVYPHNY GFIPRTLCED GDPMDVLVLM QEQVVPGCFL
RARAIGLMPM IDQGEKDDKI IAVCADDPEY RHFRDIKEIP PHRLQEIRRF FEDYKKNENK
EVAVNEFLPA EDAINAIKYS MDLYGAYIIE SLRK
