IPYR_PLAF7
ID IPYR_PLAF7 Reviewed; 380 AA.
AC O77392;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable inorganic pyrophosphatase;
DE EC=3.6.1.1;
DE AltName: Full=Pyrophosphate phospho-hydrolase;
DE Short=PPase;
GN ORFNames=MAL3P6.3, PFC0710w;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=10448855; DOI=10.1038/22964;
RA Bowman S., Lawson D., Basham D., Brown D., Chillingworth T., Churcher C.M.,
RA Craig A., Davies R.M., Devlin K., Feltwell T., Gentles S., Gwilliam R.,
RA Hamlin N., Harris D., Holroyd S., Hornsby T., Horrocks P., Jagels K.,
RA Jassal B., Kyes S., McLean J., Moule S., Mungall K.L., Murphy L.,
RA Oliver K., Quail M.A., Rajandream M.A., Rutter S., Skelton J., Squares R.,
RA Squares S., Sulston J.E., Whitehead S., Woodward J.R., Newbold C.,
RA Barrell B.G.;
RT "The complete nucleotide sequence of chromosome 3 of Plasmodium
RT falciparum.";
RL Nature 400:532-538(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474; EC=3.6.1.1;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the PPase family. {ECO:0000305}.
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DR EMBL; AL844502; CAB11148.1; -; Genomic_DNA.
DR PIR; T18509; T18509.
DR RefSeq; XP_001351233.1; XM_001351197.1.
DR PDB; 5WRU; X-ray; 3.19 A; A/B/C/D/E=1-380.
DR PDBsum; 5WRU; -.
DR AlphaFoldDB; O77392; -.
DR SMR; O77392; -.
DR STRING; 5833.PFC0710w.2; -.
DR PRIDE; O77392; -.
DR EnsemblProtists; CAB11148; CAB11148; PF3D7_0316300.1.
DR GeneID; 9221773; -.
DR KEGG; pfa:PF3D7_0316300.1; -.
DR VEuPathDB; PlasmoDB:PF3D7_0316300; -.
DR InParanoid; O77392; -.
DR PhylomeDB; O77392; -.
DR Reactome; R-PFA-379716; Cytosolic tRNA aminoacylation.
DR Reactome; R-PFA-379726; Mitochondrial tRNA aminoacylation.
DR Reactome; R-PFA-71737; Pyrophosphate hydrolysis.
DR Proteomes; UP000001450; Chromosome 3.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0006796; P:phosphate-containing compound metabolic process; IBA:GO_Central.
DR CDD; cd00412; pyrophosphatase; 1.
DR Gene3D; 3.90.80.10; -; 1.
DR InterPro; IPR008162; Pyrophosphatase.
DR InterPro; IPR036649; Pyrophosphatase_sf.
DR PANTHER; PTHR10286; PTHR10286; 1.
DR Pfam; PF00719; Pyrophosphatase; 1.
DR SUPFAM; SSF50324; SSF50324; 1.
DR PROSITE; PS00387; PPASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..380
FT /note="Probable inorganic pyrophosphatase"
FT /id="PRO_0000137573"
FT BINDING 198
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 235
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT STRAND 41..43
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 55..60
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 67..75
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 78..86
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 94..101
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 104..108
FT /evidence="ECO:0007829|PDB:5WRU"
FT TURN 110..113
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 123..129
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 165..170
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 180..183
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 203..206
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 217..230
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 233..242
FT /evidence="ECO:0007829|PDB:5WRU"
FT TURN 246..250
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 254..260
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 264..274
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 277..280
FT /evidence="ECO:0007829|PDB:5WRU"
FT HELIX 293..320
FT /evidence="ECO:0007829|PDB:5WRU"
FT TURN 356..359
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 363..367
FT /evidence="ECO:0007829|PDB:5WRU"
FT STRAND 375..377
FT /evidence="ECO:0007829|PDB:5WRU"
SQ SEQUENCE 380 AA; 45232 MW; 109FFC49AE5443B9 CRC64;
MGSKLINVEG GNNQDDNKYN SNNVISINNK VNKNDYFIET NKELKINLNF QNNNIISNIF
SNINIYDKIS NIFINNKKTY MLKYNNNINE ENFFISYFEK KDDNFVPISP WHHIDLKNDD
GTYNMIVEIT KYNYIKLEIQ LREKFNVIKQ DKKKGKLRYY HNSIYWNYGA LPQTYEYPKH
IYQNKSKKNK EALLFTGDND PLDILDIGSA CLKIGQVVPV KILGAFTLID EGELDWKIIA
INKEDKHYED INSLSDIEKY YPHTLSLLLE WFRSYKMADT KKLNLISKQL YDKKESEDLI
MKTHHYYLEF REDVKKLKEE HSKETIKEHD YVNAQNIQFN YDKLNNNDDE PMENNLLEDI
NITYYKSDSA YKPDLNIWTP
