IQCF1_HUMAN
ID IQCF1_HUMAN Reviewed; 205 AA.
AC Q8N6M8; Q8N711;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=IQ domain-containing protein F1 {ECO:0000305};
GN Name=IQCF1 {ECO:0000312|HGNC:HGNC:28607};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP LYS-76.
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=25380116; DOI=10.1111/andr.296;
RA Fang P., Xu W., Li D., Zhao X., Dai J., Wang Z., Yan X., Qin M., Zhang Y.,
RA Xu C., Wang L., Qiao Z.;
RT "A novel acrosomal protein, IQCF1, involved in sperm capacitation and the
RT acrosome reaction.";
RL Andrology 3:332-344(2015).
CC -!- FUNCTION: Involved in sperm capacitation and acrosome reaction.
CC {ECO:0000250|UniProtKB:Q9D9K8}.
CC -!- SUBUNIT: Interacts with calmodulin. {ECO:0000250|UniProtKB:Q9D9K8}.
CC -!- INTERACTION:
CC Q8N6M8-2; P55212: CASP6; NbExp=3; IntAct=EBI-21771049, EBI-718729;
CC Q8N6M8-2; P13473-2: LAMP2; NbExp=3; IntAct=EBI-21771049, EBI-21591415;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:25380116}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N6M8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N6M8-2; Sequence=VSP_024184, VSP_024185;
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DR EMBL; AC097636; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC029595; AAH29595.1; -; mRNA.
DR EMBL; BC034228; AAH34228.1; -; mRNA.
DR CCDS; CCDS2836.1; -. [Q8N6M8-1]
DR RefSeq; NP_689610.2; NM_152397.2. [Q8N6M8-1]
DR AlphaFoldDB; Q8N6M8; -.
DR BioGRID; 126306; 49.
DR IntAct; Q8N6M8; 32.
DR STRING; 9606.ENSP00000307958; -.
DR iPTMnet; Q8N6M8; -.
DR PhosphoSitePlus; Q8N6M8; -.
DR BioMuta; IQCF1; -.
DR DMDM; 229462886; -.
DR MassIVE; Q8N6M8; -.
DR PaxDb; Q8N6M8; -.
DR PeptideAtlas; Q8N6M8; -.
DR PRIDE; Q8N6M8; -.
DR ProteomicsDB; 72199; -. [Q8N6M8-1]
DR Antibodypedia; 31058; 119 antibodies from 19 providers.
DR DNASU; 132141; -.
DR Ensembl; ENST00000310914.10; ENSP00000307958.5; ENSG00000173389.16. [Q8N6M8-1]
DR GeneID; 132141; -.
DR KEGG; hsa:132141; -.
DR MANE-Select; ENST00000310914.10; ENSP00000307958.5; NM_152397.3; NP_689610.2.
DR UCSC; uc003dbv.4; human. [Q8N6M8-1]
DR CTD; 132141; -.
DR GeneCards; IQCF1; -.
DR HGNC; HGNC:28607; IQCF1.
DR HPA; ENSG00000173389; Tissue enriched (testis).
DR neXtProt; NX_Q8N6M8; -.
DR OpenTargets; ENSG00000173389; -.
DR PharmGKB; PA134895829; -.
DR VEuPathDB; HostDB:ENSG00000173389; -.
DR eggNOG; ENOG502SR3N; Eukaryota.
DR GeneTree; ENSGT00390000004641; -.
DR HOGENOM; CLU_114989_0_0_1; -.
DR InParanoid; Q8N6M8; -.
DR OMA; HSCASRG; -.
DR OrthoDB; 1354510at2759; -.
DR PhylomeDB; Q8N6M8; -.
DR TreeFam; TF337908; -.
DR PathwayCommons; Q8N6M8; -.
DR SignaLink; Q8N6M8; -.
DR BioGRID-ORCS; 132141; 7 hits in 1058 CRISPR screens.
DR GenomeRNAi; 132141; -.
DR Pharos; Q8N6M8; Tdark.
DR PRO; PR:Q8N6M8; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8N6M8; protein.
DR Bgee; ENSG00000173389; Expressed in sperm and 74 other tissues.
DR ExpressionAtlas; Q8N6M8; baseline and differential.
DR Genevisible; Q8N6M8; HS.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0005516; F:calmodulin binding; IBA:GO_Central.
DR GO; GO:2000344; P:positive regulation of acrosome reaction; ISS:UniProtKB.
DR GO; GO:0060474; P:positive regulation of flagellated sperm motility involved in capacitation; ISS:UniProtKB.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR039887; IQCF.
DR PANTHER; PTHR21633; PTHR21633; 1.
DR Pfam; PF00612; IQ; 2.
DR SMART; SM00015; IQ; 2.
DR PROSITE; PS50096; IQ; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Reference proteome; Repeat.
FT CHAIN 1..205
FT /note="IQ domain-containing protein F1"
FT /id="PRO_0000282556"
FT DOMAIN 68..97
FT /note="IQ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 124..153
FT /note="IQ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 58..81
FT /note="PPENQKKLSDKDTVATKIQAWWRG -> VCQWNMVIVSMDWKSLESEDLRNL
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024184"
FT VAR_SEQ 82..205
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024185"
FT VARIANT 76
FT /note="Q -> K (in dbSNP:rs17852683)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_055096"
FT VARIANT 114
FT /note="R -> Q (in dbSNP:rs11927897)"
FT /id="VAR_031414"
SQ SEQUENCE 205 AA; 23699 MW; B13B0E3573927D19 CRC64;
MEEKQPQKTK EPSKEDEPQQ KEMPTHLSLG AESKAEAKTP VLVETQTVDN ANEKSEKPPE
NQKKLSDKDT VATKIQAWWR GTLVRRALLH AALSACIIQC WWRLILSKIL KKRRQAALEA
FSRKEWAAVT LQSQARMWRI RRRYCQVLNA VRIIQAYWRC RSCASRGFIK GQYRVTANQL
HLQLEILLDS GPCIVTECIP FSIKE
