ID   IQD33_ARATH             Reviewed;         442 AA.
AC   Q8L8M9; Q9FKI3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Protein IQ-DOMAIN 33 {ECO:0000303|PubMed:16368012};
DE            Short=AtIQD33 {ECO:0000303|PubMed:16368012};
GN   Name=IQD33 {ECO:0000303|PubMed:16368012};
GN   OrderedLocusNames=At5g35670 {ECO:0000312|Araport:AT5G35670};
GN   ORFNames=MXH1.1 {ECO:0000312|EMBL:BAB09264.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INTERACTION WITH CALMODULIN, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=16368012; DOI=10.1186/1471-2148-5-72;
RA   Abel S., Savchenko T., Levy M.;
RT   "Genome-wide comparative analysis of the IQD gene families in Arabidopsis
RT   thaliana and Oryza sativa.";
RL   BMC Evol. Biol. 5:72-72(2005).
RN   [6]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH CALMODULIN.
RC   STRAIN=cv. Columbia;
RX   PubMed=28115582; DOI=10.1104/pp.16.01743;
RA   Buerstenbinder K., Moeller B., Ploetner R., Stamm G., Hause G., Mitra D.,
RA   Abel S.;
RT   "The IQD family of calmodulin-binding proteins links calcium signaling to
RT   microtubules, membrane subdomains, and the nucleus.";
RL   Plant Physiol. 173:1692-1708(2017).
RN   [7]
RP   REVIEW.
RX   PubMed=28534650; DOI=10.1080/15592324.2017.1331198;
RA   Buerstenbinder K., Mitra D., Quegwer J.;
RT   "Functions of IQD proteins as hubs in cellular calcium and auxin signaling:
RT   A toolbox for shape formation and tissue-specification in plants?";
RL   Plant Signal. Behav. 12:E1331198-E1331198(2017).
CC   -!- FUNCTION: May be involved in cooperative interactions with calmodulins
CC       or calmodulin-like proteins (By similarity). Recruits calmodulin
CC       proteins to microtubules, thus being a potential scaffold in cellular
CC       signaling and trafficking (By similarity). May associate with nucleic
CC       acids and regulate gene expression at the transcriptional or post-
CC       transcriptional level (By similarity). {ECO:0000250|UniProtKB:Q9SF32}.
CC   -!- SUBUNIT: Binds to multiple calmodulin (CaM) in the presence of Ca(2+)
CC       and CaM-like proteins. {ECO:0000250|UniProtKB:Q9SF32}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC       ECO:0000269|PubMed:28115582}. Note=Recruits calmodulin (CaM2) at the
CC       nucleus. {ECO:0000269|PubMed:28115582}.
CC   -!- SIMILARITY: Belongs to the IQD family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB09264.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB011485; BAB09264.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED93999.1; -; Genomic_DNA.
DR   EMBL; AK175704; BAD43467.1; -; mRNA.
DR   EMBL; AY088904; AAM67210.1; -; mRNA.
DR   RefSeq; NP_568529.1; NM_122958.4.
DR   AlphaFoldDB; Q8L8M9; -.
DR   STRING; 3702.AT5G35670.1; -.
DR   PaxDb; Q8L8M9; -.
DR   PRIDE; Q8L8M9; -.
DR   ProteomicsDB; 191526; -.
DR   EnsemblPlants; AT5G35670.1; AT5G35670.1; AT5G35670.
DR   GeneID; 833540; -.
DR   Gramene; AT5G35670.1; AT5G35670.1; AT5G35670.
DR   KEGG; ath:AT5G35670; -.
DR   Araport; AT5G35670; -.
DR   TAIR; locus:2177266; AT5G35670.
DR   eggNOG; ENOG502QSU8; Eukaryota.
DR   HOGENOM; CLU_059472_0_0_1; -.
DR   InParanoid; Q8L8M9; -.
DR   OMA; CHRIQRR; -.
DR   OrthoDB; 945638at2759; -.
DR   PhylomeDB; Q8L8M9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q8L8M9; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR   Pfam; PF00612; IQ; 1.
DR   PROSITE; PS50096; IQ; 1.
PE   1: Evidence at protein level;
KW   Calmodulin-binding; Nucleus; Reference proteome.
FT   CHAIN           1..442
FT                   /note="Protein IQ-DOMAIN 33"
FT                   /id="PRO_0000453136"
FT   DOMAIN          159..188
FT                   /note="IQ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   REGION          184..212
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          270..282
FT                   /note="Calmodulin-binding"
FT                   /evidence="ECO:0000303|PubMed:16368012"
FT   REGION          375..442
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           385..392
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT   COMPBIAS        196..212
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        375..389
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        405..442
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   442 AA;  49544 MW;  8912A64F4538FE03 CRC64;
     MGVTGGLVRS IFFRNKSFGA HDYNNGRGNL GEKKRWSSVR SYLCGDEFNS VRAVNDSESI
     KDSEALLTMS QQLSSEFGMP FGSVIAIEDS ASVRHLEDED SGSVKSSEAT VTQPLLEEKS
     KGLDCYLKEE DVEDNQSEAT ETHIPKKHQT TPISKLFLEE DAAVIIQSAF RSYLAIRRSK
     EEEETFAKEE SFSGEESQDN ASMGTSLEAQ TGNSVKAPFF RRKRVSANRR TLQKNNTQVL
     RIKEDWDDST VSSTISKSRI QSRVEAMTKR ERALAYAFSQ QLRICSKKKQ IDRSSEDDSN
     IGWSWLERWM ATRVPDSIPI EPRTDVATKN QSLIRKNRAF GTAGELESCA SNDLPLHFES
     ISETQGDATT VLQTEKSSFK PSISKRKSVP SYKSQRKHHK LQATKSDLQQ QTKKAKKAKT
     TPTSCKTGNE CEETSHKLNS ST