APPX_ECOLI
ID APPX_ECOLI Reviewed; 30 AA.
AC P24244; A5A610;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Putative cytochrome bd-II ubiquinol oxidase subunit AppX;
GN Name=appX; Synonyms=yccB; OrderedLocusNames=b4592, JW0961.1;
GN ORFNames=b0979.1;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2168385; DOI=10.1128/jb.172.9.5497-5500.1990;
RA Dassa J., Marck C., Boquet P.L.;
RT "The complete nucleotide sequence of the Escherichia coli gene appA reveals
RT significant homology between pH 2.5 acid phosphatase and glucose-1-
RT phosphatase.";
RL J. Bacteriol. 172:5497-5500(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1658595; DOI=10.1007/bf00267454;
RA Dassa J., Fsihi H., Marck C., Dion M., Kieffer-Bontemps M., Boquet P.L.;
RT "A new oxygen-regulated operon in Escherichia coli comprises the genes for
RT a putative third cytochrome oxidase and for pH 2.5 acid phosphatase
RT (appA).";
RL Mol. Gen. Genet. 229:341-352(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19121005; DOI=10.1111/j.1365-2958.2008.06495.x;
RA Hemm M.R., Paul B.J., Schneider T.D., Storz G., Rudd K.E.;
RT "Small membrane proteins found by comparative genomics and ribosome binding
RT site models.";
RL Mol. Microbiol. 70:1487-1501(2008).
RN [6]
RP INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19734316; DOI=10.1128/jb.00872-09;
RA Hemm M.R., Paul B.J., Miranda-Rios J., Zhang A., Soltanzad N., Storz G.;
RT "Small stress response proteins in Escherichia coli: proteins missed by
RT classical proteomic studies.";
RL J. Bacteriol. 192:46-58(2010).
RN [7]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=21778229; DOI=10.1074/jbc.m111.245696;
RA Fontaine F., Fuchs R.T., Storz G.;
RT "Membrane localization of small proteins in Escherichia coli.";
RL J. Biol. Chem. 286:32464-32474(2011).
RN [8]
RP FUNCTION, POSSIBLE INTERACTION WITH CYDA AND CYDB, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=23749980; DOI=10.1128/jb.00324-13;
RA Vanorsdel C.E., Bhatt S., Allen R.J., Brenner E.P., Hobson J.J., Jamil A.,
RA Haynes B.M., Genson A.M., Hemm M.R.;
RT "The Escherichia coli CydX protein is a member of the CydAB cytochrome bd
RT oxidase complex and is required for cytochrome bd oxidase activity.";
RL J. Bacteriol. 195:3640-3650(2013).
CC -!- FUNCTION: Might be part of cytochrome bd-II oxidase (appB and appC).
CC Able to restore reductant resistance to a cydX deletion mutant upon
CC overexpression. CydX and this protein may have some functional overlap.
CC {ECO:0000269|PubMed:23749980}.
CC -!- SUBUNIT: Able to interact with CydA and CydB upon overexpression.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}. Note=In sucrose cushions fractionates
CC with the outer membrane, in sarcosyl fractionation with the inner
CC membrane. May have its C-terminus in the cytoplasm (PubMed:21778229).
CC {ECO:0000269|PubMed:21778229}.
CC -!- INDUCTION: Expressed during stationary phase (PubMed:19121005) and in
CC minimal glycerol medium, at 45 degrees Celsius, strongly (30-fold)
CC induced in low oxygen (PubMed:19734316) (at protein level).
CC {ECO:0000269|PubMed:19121005, ECO:0000269|PubMed:19734316}.
CC -!- DISRUPTION PHENOTYPE: Not essential, no change in sensitivity to
CC reductant (beta-mercaptoethanol). {ECO:0000269|PubMed:23749980}.
CC -!- SIMILARITY: Belongs to the cytochrome ubiquinol oxidase subunit X
CC family. {ECO:0000305}.
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DR EMBL; M58708; AAA72085.1; -; Genomic_DNA.
DR EMBL; S63811; AAB20287.1; -; Genomic_DNA.
DR EMBL; U00096; ABP93439.1; -; Genomic_DNA.
DR EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A36733; A36733.
DR RefSeq; WP_000270305.1; NZ_STEB01000006.1.
DR RefSeq; YP_001165313.1; NC_000913.3.
DR PDB; 7OSE; EM; 3.00 A; C/F=1-30.
DR PDBsum; 7OSE; -.
DR AlphaFoldDB; P24244; -.
DR SMR; P24244; -.
DR STRING; 511145.b4592; -.
DR TCDB; 3.D.4.3.2; the proton-translocating cytochrome oxidase (cox) superfamily.
DR PaxDb; P24244; -.
DR PRIDE; P24244; -.
DR EnsemblBacteria; ABP93439; ABP93439; b4592.
DR GeneID; 66670744; -.
DR GeneID; 948955; -.
DR KEGG; eco:b4592; -.
DR PATRIC; fig|511145.12.peg.1014; -.
DR EchoBASE; EB1105; -.
DR PhylomeDB; P24244; -.
DR BioCyc; EcoCyc:MON0-2817; -.
DR BioCyc; MetaCyc:MON0-2817; -.
DR PRO; PR:P24244; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR012994; YbgT_YccB.
DR Pfam; PF08173; YbgT_YccB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Stress response; Transmembrane; Transmembrane helix.
FT CHAIN 1..30
FT /note="Putative cytochrome bd-II ubiquinol oxidase subunit
FT AppX"
FT /id="PRO_0000168781"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..24
FT /evidence="ECO:0007829|PDB:7OSE"
FT TURN 25..27
FT /evidence="ECO:0007829|PDB:7OSE"
SQ SEQUENCE 30 AA; 3597 MW; 9E27FCD533329606 CRC64;
MWYLLWFVGI LLMCSLSTLV LVWLDPRLKS
