APPY_ECOLI
ID APPY_ECOLI Reviewed; 249 AA.
AC P05052; Q2MBL7;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 25-MAY-2022, entry version 159.
DE RecName: Full=HTH-type transcriptional regulator AppY;
DE AltName: Full=M5 polypeptide;
GN Name=appY; OrderedLocusNames=b0564, JW0553;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / ATCC 35607 / JM83;
RX PubMed=3295784; DOI=10.1093/nar/15.9.3924;
RA Kemp E.H., Minton N.P., Mann N.H.;
RT "Complete nucleotide sequence and deduced amino acid sequence of the M5
RT polypeptide gene of Escherichia coli.";
RL Nucleic Acids Res. 15:3924-3924(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2537825; DOI=10.1128/jb.171.3.1683-1691.1989;
RA Atlung T., Nielsen A., Hansen F.G.;
RT "Isolation, characterization, and nucleotide sequence of appY, a regulatory
RT gene for growth-phase-dependent gene expression in Escherichia coli.";
RL J. Bacteriol. 171:1683-1691(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP CHARACTERIZATION.
RX PubMed=8071219; DOI=10.1128/jb.176.17.5414-5422.1994;
RA Atlung T., Brondsted L.;
RT "Role of the transcriptional activator AppY in regulation of the cyx appA
RT operon of Escherichia coli by anaerobiosis, phosphate starvation, and
RT growth phase.";
RL J. Bacteriol. 176:5414-5422(1994).
RN [7]
RP FUNCTION IN THE STABILIZATION OF RPOS.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=18383615; DOI=10.1111/j.1365-2958.2008.06146.x;
RA Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S.;
RT "Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia
RT coli via the action of multiple anti-adaptors.";
RL Mol. Microbiol. 68:298-313(2008).
CC -!- FUNCTION: Induces the synthesis of acid phosphatase (AppA) and several
CC other polypeptides (such as AppBC) during the deceleration phase of
CC growth. It also acts as a transcriptional repressor for one group of
CC proteins that are synthesized preferentially in exponential growth and
CC for one group synthesized only in the stationary phase. Also involved
CC in the stabilization of the sigma stress factor RpoS during stress
CC conditions. {ECO:0000269|PubMed:18383615}.
CC -!- MISCELLANEOUS: Encoded by the cryptic lambdoid prophage DLP12.
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DR EMBL; Y00138; CAA68331.1; -; Genomic_DNA.
DR EMBL; M24530; AAA23454.1; -; Genomic_DNA.
DR EMBL; U82598; AAB40760.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73665.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76339.1; -; Genomic_DNA.
DR PIR; A94498; BVECM5.
DR RefSeq; NP_415096.1; NC_000913.3.
DR RefSeq; WP_000386784.1; NZ_LN832404.1.
DR AlphaFoldDB; P05052; -.
DR SMR; P05052; -.
DR BioGRID; 4263522; 5.
DR BioGRID; 853080; 1.
DR IntAct; P05052; 26.
DR STRING; 511145.b0564; -.
DR jPOST; P05052; -.
DR PaxDb; P05052; -.
DR PRIDE; P05052; -.
DR EnsemblBacteria; AAC73665; AAC73665; b0564.
DR EnsemblBacteria; BAE76339; BAE76339; BAE76339.
DR GeneID; 948797; -.
DR KEGG; ecj:JW0553; -.
DR KEGG; eco:b0564; -.
DR PATRIC; fig|511145.12.peg.587; -.
DR EchoBASE; EB0048; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_000445_81_4_6; -.
DR InParanoid; P05052; -.
DR OMA; GEFSAIC; -.
DR PhylomeDB; P05052; -.
DR BioCyc; EcoCyc:PD00967; -.
DR PRO; PR:P05052; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IDA:EcoCyc.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0010165; P:response to X-ray; IMP:EcoCyc.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..249
FT /note="HTH-type transcriptional regulator AppY"
FT /id="PRO_0000194497"
FT DOMAIN 139..236
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 156..177
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 203..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT CONFLICT 26..31
FT /note="Missing (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 28763 MW; 3985FF0ECCF03D8A CRC64;
MDYVCSVVFI CQSFDLIINR RVISFKKNSL FIVSDKIRRE LPVCPSKLRI VDIDKKTCLS
FFIDVNNELP GKFTLDKNGY IAEEEPPLSL VFSLFEGIKI ADSHSLWLKE RLCISLLAMF
KKRESVNSFI LTNINTFTCK ITGIISFNIE RQWHLKDIAE LIYTSESLIK KRLRDEGTSF
TEILRDTRMR YAKKLITSNS YSINVVAQKC GYNSTSYFIC AFKDYYGVTP SHYFEKIIGV
TDGINKTID
