IRA1_YEAST
ID IRA1_YEAST Reviewed; 3092 AA.
AC P18963; D6VQD6;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Inhibitory regulator protein IRA1;
GN Name=IRA1; Synonyms=GLC1, PPD1; OrderedLocusNames=YBR140C;
GN ORFNames=YBR1016;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7754712; DOI=10.1002/yea.320100911;
RA Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A.,
RA Slonimski P.P., Sokolowska B., Herbert C.J.;
RT "The sequence of 12.5 kb from the right arm of chromosome II predicts a new
RT N-terminal sequence for the IRA1 protein and reveals two new genes, one of
RT which is a DEAD-box helicase.";
RL Yeast 10:1227-1234(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
RX PubMed=2540426; DOI=10.1128/mcb.9.2.757-768.1989;
RA Tanaka K., Matsumoto K., Toh-e A.;
RT "IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in
RT Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 9:757-768(1989).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091856; DOI=10.1002/yea.320100002;
RA Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F.,
RA Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M.,
RA Herbert C.J.;
RT "The sequence of 29.7 kb from the right arm of chromosome II reveals 13
RT complete open reading frames, of which ten correspond to new genes.";
RL Yeast 10:S1-S11(1994).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-915 AND SER-1342,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway in
CC S.cerevisiae. Stimulates the GTPase activity of Ras proteins.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
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DR EMBL; Z36009; CAA85098.1; -; Genomic_DNA.
DR EMBL; M24378; AAA34709.1; -; Genomic_DNA.
DR EMBL; X75891; CAA53498.1; -; Genomic_DNA.
DR EMBL; X78937; CAA55537.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07256.1; -; Genomic_DNA.
DR PIR; S46009; S46009.
DR RefSeq; NP_009698.3; NM_001178488.3.
DR SMR; P18963; -.
DR BioGRID; 32840; 59.
DR DIP; DIP-2318N; -.
DR IntAct; P18963; 5.
DR MINT; P18963; -.
DR STRING; 4932.YBR140C; -.
DR iPTMnet; P18963; -.
DR MaxQB; P18963; -.
DR PaxDb; P18963; -.
DR PRIDE; P18963; -.
DR EnsemblFungi; YBR140C_mRNA; YBR140C; YBR140C.
DR GeneID; 852437; -.
DR KEGG; sce:YBR140C; -.
DR SGD; S000000344; IRA1.
DR VEuPathDB; FungiDB:YBR140C; -.
DR eggNOG; KOG1826; Eukaryota.
DR GeneTree; ENSGT00940000176574; -.
DR HOGENOM; CLU_000439_0_0_1; -.
DR InParanoid; P18963; -.
DR OMA; RLYNIVF; -.
DR BioCyc; YEAST:G3O-29094-MON; -.
DR Reactome; R-SCE-9696273; RND1 GTPase cycle.
DR PRO; PR:P18963; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P18963; protein.
DR GO; GO:0016020; C:membrane; IMP:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005096; F:GTPase activator activity; IMP:SGD.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IMP:SGD.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:SGD.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IMP:SGD.
DR GO; GO:0045761; P:regulation of adenylate cyclase activity; IMP:SGD.
DR Gene3D; 1.10.506.10; -; 1.
DR Gene3D; 3.40.525.10; -; 1.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR039360; Ras_GTPase.
DR InterPro; IPR023152; RasGAP_CS.
DR InterPro; IPR001936; RasGAP_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR PANTHER; PTHR10194; PTHR10194; 1.
DR Pfam; PF00616; RasGAP; 2.
DR SMART; SM00323; RasGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS00509; RAS_GTPASE_ACTIV_1; 1.
DR PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; GTPase activation; Phosphoprotein; Reference proteome.
FT CHAIN 1..3092
FT /note="Inhibitory regulator protein IRA1"
FT /id="PRO_0000056657"
FT DOMAIN 1709..1898
FT /note="Ras-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT REGION 375..430
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1003..1023
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 951..988
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 497
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 915
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 1342
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 1753
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
FT MOD_RES 3004
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
FT CONFLICT 361
FT /note="V -> G (in Ref. 4; AAA34709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 3092 AA; 350980 MW; 291339AE5BD26E08 CRC64;
MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN SCRSIILNIA
ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV LLRLLSDVFE YYWDQNNDFK
KIRNDNYKPG FSSHRPNFHT SRPKHTRINP ALATMLLCKI SKLKFNTRTL KVLQNMSHHL
SGSATISKSS ILPDSQEFLQ KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA
PLLISHTSTE LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA
FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL LFDDVYSTFS
VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK QSSVNASGNV SPSQFSTGND
ASPTSPMASL SSPLNTNILG YPLSPITSTL GQANTSTSTT AATTKTDADT PSTMNTNNNN
NNNNSANLNN IPQRIFSLDD ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG
VNNSQSQNDQ SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY
RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK FLTYLIRNLN
GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS KRFYNLLGQN LEVGTNWNSA
TANTFISHCV ERNPLTHRRL QLEFFASGLQ LDSDLFLRHL QLEKELNHID LPKISLYTEG
FRVFFHLVST KKLHEDIAEK TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL
DQFDAARTLS NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR
TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT TKRYASPNDS
ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA TPTILKNIKS PKPNKTKKIA
DDKQLKQPSY SRVILSDNDE ARKIMMNIFS IFKRMTNWFI RPDANTEFPK TFTDIIKPLF
VSILDSNQRL QVTARAFIEI PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD
LKLENAKREM LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS
LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD NFTASGSVAL
QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT QEELVDFRSL AGILASMSGI
LSDMQELEKS KSAPDNEGDS LSFESRNPAY EVHKSLKLEL TKKMNFFISK QCQWLNNPNL
LTRENSRDIL SIELHPLSFN LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI
LKRDDDEKIM LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG
ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY IDTSIESAKA
LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL EKSADLNQFP VSLRHKISIL
NENVIIALTN LSNANVNVSL KFTLPMGYSP NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK
MLAIDDFLKY IIKNPILAFF GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK
RAARSDDILR RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN
SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY IAVGSFVFLR
FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR ENIFKKDILV SKEEFLKTCS
DKIFNFLSEL CKIPTNNFTV NVREDPTPIS FDYSFLHKFF YLNEFTIRKE IINESKLPGE
FSFLKNTVML NDKILGVLGQ PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE
EEDNAPFVHE AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT
TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV ENPYLVTTIP
RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD KEKKKFCPVS LKIGNKYFQV
LHEIPQLYKV TVSNRTFSIK FNNVYKISNL ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP
KYLEIVKMFY YAQLKMEEDF GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV
GLFNEDDLVK NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST
ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG PEAISRIIYS
LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH ALDRDSENRD WMKAVSILTS
FPTTEIACQV IEKLINMIKS FLPSLAVEAS AHSWSELTIL SKISVSIFFE SPLLSQMYLP
EILFAVSLLI DVGPSEIRVS LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF
ISGFSQEKGR VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA
IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM FIIIAHVFTY
SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM VNCLKRLYTV HLQLGFDGKS
LAKKLMESRN FAATLLAKLE SYNGCIWNED NFPHIILGFI ANGLSIPVVK GAALDCLQAL
FKNTYYERKS NPKSSDYLCY LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI
NWLSSDSDKS NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF
RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV VKPLDIFDQE
HIEKLKGEGE HQVAIYERKR LATMILARMS CS
