IRAM_ECOLI
ID IRAM_ECOLI Reviewed; 107 AA.
AC P75987; Q2MBH3;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Anti-adapter protein IraM;
DE AltName: Full=Enhancing lycopene biosynthesis protein 1;
GN Name=iraM; Synonyms=elb1, elbA, ycgW; OrderedLocusNames=b1160, JW1147;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP POSSIBLE FUNCTION.
RX PubMed=9603997; DOI=10.1093/oxfordjournals.jbchem.a022047;
RA Hemmi H., Ohnuma S., Nagaoka K., Nishino T.;
RT "Identification of genes affecting lycopene formation in Escherichia coli
RT transformed with carotenoid biosynthetic genes: candidates for early genes
RT in isoprenoid biosynthesis.";
RL J. Biochem. 123:1088-1096(1998).
RN [4]
RP MUTATOR PHENOTYPE.
RX PubMed=15225322; DOI=10.1111/j.1365-2958.2004.04125.x;
RA Yang H., Wolff E., Kim M., Diep A., Miller J.H.;
RT "Identification of mutator genes and mutational pathways in Escherichia
RT coli using a multicopy cloning approach.";
RL Mol. Microbiol. 53:283-295(2004).
RN [5]
RP FUNCTION IN THE STABILIZATION OF RPOS, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=18383615; DOI=10.1111/j.1365-2958.2008.06146.x;
RA Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S.;
RT "Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia
RT coli via the action of multiple anti-adaptors.";
RL Mol. Microbiol. 68:298-313(2008).
CC -!- FUNCTION: Inhibits RpoS proteolysis by regulating RssB activity,
CC thereby increasing the stability of the sigma stress factor RpoS during
CC magnesium starvation. May also be involved in the early steps of
CC isoprenoid biosynthesis, possibly through its role as RssB regulator.
CC {ECO:0000269|PubMed:18383615}.
CC -!- INTERACTION:
CC P75987; P0AEV1: rssB; NbExp=2; IntAct=EBI-6479798, EBI-1122979;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By magnesium and calcium starvation, via the transcriptional
CC regulator PhoP. {ECO:0000269|PubMed:18383615}.
CC -!- SIMILARITY: Belongs to the IraM/RssC family. {ECO:0000305}.
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DR EMBL; U00096; AAC74244.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76383.1; -; Genomic_DNA.
DR PIR; E64861; E64861.
DR RefSeq; NP_415678.1; NC_000913.3.
DR RefSeq; WP_001295666.1; NZ_SSZK01000010.1.
DR AlphaFoldDB; P75987; -.
DR SMR; P75987; -.
DR BioGRID; 4262861; 12.
DR IntAct; P75987; 1.
DR STRING; 511145.b1160; -.
DR PaxDb; P75987; -.
DR PRIDE; P75987; -.
DR EnsemblBacteria; AAC74244; AAC74244; b1160.
DR EnsemblBacteria; BAE76383; BAE76383; BAE76383.
DR GeneID; 945729; -.
DR KEGG; ecj:JW1147; -.
DR KEGG; eco:b1160; -.
DR PATRIC; fig|83333.103.peg.1949; -.
DR EchoBASE; EB3644; -.
DR eggNOG; ENOG50331R2; Bacteria.
DR HOGENOM; CLU_143527_1_0_6; -.
DR OMA; FSAIWGD; -.
DR PhylomeDB; P75987; -.
DR BioCyc; EcoCyc:G6600-MON; -.
DR PRO; PR:P75987; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043856; F:anti-sigma factor antagonist activity; IDA:EcoCyc.
DR GO; GO:0071468; P:cellular response to acidic pH; IMP:EcoCyc.
DR GO; GO:0010350; P:cellular response to magnesium starvation; IMP:EcoCyc.
DR GO; GO:0042177; P:negative regulation of protein catabolic process; IDA:EcoCyc.
DR Gene3D; 2.40.50.650; -; 1.
DR HAMAP; MF_01199; Anti_adapt_IraM; 1.
DR InterPro; IPR014448; Anti-adapter_IraM.
DR InterPro; IPR038679; PmrD_sf.
DR PIRSF; PIRSF007036; Elb1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Stress response.
FT CHAIN 1..107
FT /note="Anti-adapter protein IraM"
FT /id="PRO_0000086951"
SQ SEQUENCE 107 AA; 12133 MW; 27F8A8C64FA18984 CRC64;
MKWIVIDTVI QPTCGISFSA IWGNMKMIIW YQSTIFLPPG SIFTPVKSGI ILKDKEYPIT
IYHIAPFNKD LWSLLKSSQE CPPGESKITN KCLHNSCIIK ICPYGLK
