APRA_DICDI
ID APRA_DICDI Reviewed; 494 AA.
AC Q5XM24; Q54TM5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Autocrine proliferation repressor protein A;
DE AltName: Full=PhoPQ-activated pathogenicity-related protein;
DE Flags: Precursor;
GN Name=aprA; ORFNames=DDB_G0281663;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-31; 379-389 AND
RP 435-456, FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=16176950; DOI=10.1242/dev.02032;
RA Brock D.A., Gomer R.H.;
RT "A secreted factor represses cell proliferation in Dictyostelium.";
RL Development 132:4553-4562(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
RN [4]
RP INTERACTION WITH CFAD.
RX PubMed=18611962; DOI=10.1242/jcs.026682;
RA Bakthavatsalam D., Brock D.A., Nikravan N.N., Houston K.D., Hatton R.D.,
RA Gomer R.H.;
RT "The secreted Dictyostelium protein CfaD is a chalone.";
RL J. Cell Sci. 121:2473-2480(2008).
RN [5]
RP ERRATUM OF PUBMED:18611962.
RA Bakthavatsalam D., Brock D.A., Nikravan N.N., Houston K.D., Hatton R.D.,
RA Gomer R.H.;
RL J. Cell Sci. 121:2782-2782(2008).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=18550798; DOI=10.1091/mbc.e08-01-0075;
RA Calvo-Garrido J., Carilla-Latorre S., Lazaro-Dieguez F., Egea G.,
RA Escalante R.;
RT "Vacuole membrane protein 1 is an endoplasmic reticulum protein required
RT for organelle biogenesis, protein secretion, and development.";
RL Mol. Biol. Cell 19:3442-3453(2008).
RN [7]
RP FUNCTION, INTERACTION WITH CFAD, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19187549; DOI=10.1186/1471-2091-10-4;
RA Choe J.M., Bakthavatsalam D., Phillips J.E., Gomer R.H.;
RT "Dictyostelium cells bind a secreted autocrine factor that represses cell
RT proliferation.";
RL BMC Biochem. 10:4-4(2009).
CC -!- FUNCTION: Inhibitor that slows proliferation of secreting cells (also
CC known as chalone). May function by binding to cell surface receptors.
CC Requires cfaD for activity. Overexpression slows proliferation.
CC {ECO:0000269|PubMed:16176950, ECO:0000269|PubMed:19187549}.
CC -!- SUBUNIT: Interacts with cfaD. {ECO:0000269|PubMed:18611962,
CC ECO:0000269|PubMed:19187549}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18550798,
CC ECO:0000269|PubMed:19187549}.
CC -!- DEVELOPMENTAL STAGE: Expressed at highest levels in vegetatively
CC growing cells and declines during development (at protein level).
CC {ECO:0000269|PubMed:16176950}.
CC -!- INDUCTION: Up-regulated by phagocytic stimuli.
CC {ECO:0000269|PubMed:18559084}.
CC -!- DISRUPTION PHENOTYPE: Cells proliferate more rapidly than wild type
CC cells and exhibit supernumerary centrosomes and a cytokinesis defect.
CC They also produce fewer spores at culmination.
CC {ECO:0000269|PubMed:16176950, ECO:0000269|PubMed:19187549}.
CC -!- SIMILARITY: Belongs to the pqaA family. {ECO:0000305}.
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DR EMBL; AY750687; AAU95081.1; -; Genomic_DNA.
DR EMBL; AAFI02000042; EAL66593.1; -; Genomic_DNA.
DR RefSeq; XP_640566.1; XM_635474.1.
DR AlphaFoldDB; Q5XM24; -.
DR STRING; 44689.DDB0231036; -.
DR ESTHER; dicdi-apra; PhoPQ_related.
DR MEROPS; S82.001; -.
DR PaxDb; Q5XM24; -.
DR EnsemblProtists; EAL66593; EAL66593; DDB_G0281663.
DR GeneID; 8623176; -.
DR KEGG; ddi:DDB_G0281663; -.
DR dictyBase; DDB_G0281663; aprA.
DR eggNOG; ENOG502QU2G; Eukaryota.
DR HOGENOM; CLU_036488_1_0_1; -.
DR InParanoid; Q5XM24; -.
DR OMA; VPKYIVN; -.
DR PhylomeDB; Q5XM24; -.
DR PRO; PR:Q5XM24; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0032991; C:protein-containing complex; IDA:dictyBase.
DR GO; GO:0045499; F:chemorepellent activity; IDA:dictyBase.
DR GO; GO:0005096; F:GTPase activator activity; IDA:dictyBase.
DR GO; GO:0008233; F:peptidase activity; IDA:dictyBase.
DR GO; GO:0005102; F:signaling receptor binding; IDA:dictyBase.
DR GO; GO:0090630; P:activation of GTPase activity; IDA:dictyBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0050919; P:negative chemotaxis; IDA:dictyBase.
DR GO; GO:1903665; P:negative regulation of asexual reproduction; IMP:dictyBase.
DR GO; GO:0072659; P:protein localization to plasma membrane; IDA:dictyBase.
DR GO; GO:0031157; P:regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR009199; PhoPQ-act_pathogen-rel_PqaA.
DR PANTHER; PTHR31497; PTHR31497; 1.
DR Pfam; PF10142; PhoPQ_related; 1.
DR PIRSF; PIRSF014728; PqaA; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Direct protein sequencing; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000269|PubMed:16176950"
FT CHAIN 19..494
FT /note="Autocrine proliferation repressor protein A"
FT /id="PRO_0000328552"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 494 AA; 55402 MW; CEBB6B6520FFA6F8 CRC64;
MSKLLILLLL SLVASIFSTP LDDYVNAPDD TYKWTLNNTI EYETFTGYIL ELTSQTWMAE
KSDWPVWKHW VSICVPKGVT TTTTFIYVDG GSNDNWKVPG SMDQTIEIVC LSSGSVSVGL
TQIPNQPIIF NNDGVQRFED DLVAYTWRQF LGNTSEPLWL ARLPMTKAVV KCMDAVQEFG
KTIGYNSENF VIAGASKRGW TTWLAGVVDP RIIAIVPIVM PILNMIPNMG HQFYAYGEWS
FALNDYTGQG VMDYLNGPQM VELAAIVDPF SYRDRYTMPI YAIASSDDEF FLPDSPQFFW
NNLTATPEKH LRIVPNAEHS LMGHQIDIIL SIVTFVRLLI TNQPRPTFTW DITYSEDLNS
GTIVLTVPEG GIIPYKVKVW TAVTESTTRR DFRIITCMDI TKCIQFIIWD PSDITPTSTG
VYSITLSKPD AGWRAFFLEA EYLYAKNSID DEYTLKFTSE VAIVPNTLPF GSCSEYNACG
DGSQGDSASS TATL
