ID   4HYPE_BURCH             Reviewed;         310 AA.
AC   A0AZQ0;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=4-hydroxyproline epimerase;
DE            EC=5.1.1.8;
DE   AltName: Full=Hydroxyproline-2-epimerase;
DE            Short=HyPRE;
GN   OrderedLocusNames=Bcen2424_4140;
OS   Burkholderia cenocepacia (strain HI2424).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=331272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI2424;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., LiPuma J.J., Gonzalez C.F.,
RA   Konstantinidis K., Tiedje J.M., Richardson P.;
RT   "Complete sequence of chromosome 2 of Burkholderia cenocepacia HI2424.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Allows intracellular utilization of 4-hydroxyproline, one of
CC       the major constituents of host collagen, by converting 4-hydroxy-L-
CC       proline to 4-hydroxy-D-proline, which can be further metabolized by
CC       intracellular 4-hydroxy-D-proline oxidases. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline;
CC         Xref=Rhea:RHEA:21152, ChEBI:CHEBI:57690, ChEBI:CHEBI:58375;
CC         EC=5.1.1.8;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK10876.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000459; ABK10876.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_063828181.1; NC_008543.1.
DR   AlphaFoldDB; A0AZQ0; -.
DR   SMR; A0AZQ0; -.
DR   KEGG; bch:Bcen2424_4140; -.
DR   HOGENOM; CLU_036729_1_0_4; -.
DR   OrthoDB; 559014at2; -.
DR   GO; GO:0047580; F:4-hydroxyproline epimerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   3: Inferred from homology;
KW   Isomerase.
FT   CHAIN           1..310
FT                   /note="4-hydroxyproline epimerase"
FT                   /id="PRO_0000354033"
FT   ACT_SITE        88
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   ACT_SITE        236
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         89..90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         208
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         237..238
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
SQ   SEQUENCE   310 AA;  32684 MW;  8A5DDDFE72E5C963 CRC64;
     MKRIQIIDSH TGGEPTRLVV SGFPSLGRGT MAERRDVLAR EHDRYRTACI LEPRGSDVMV
     GALLCDPVAP DAAAGVIFFN NSGYLGMCGH GTIGVVRTLH HMGRIEPGVH RIETPVGTVE
     ATLHDDLSVS VRNVPAYRHA QGVVLDVPGY GPVKGDIAWG GNWFFLISDH GQRVAGDNVA
     ALTAYASAVR EGLERAGITG ANGGEIDHIE LFADDPEHDS RSFVLCPGLA YDRSPCGTGT
     SAKLACLAAD GKLAPGVVWR QASVIGSVFH ASYELADGGI VPTIRGSAHL SAEATLLIDE
     DDPFGWGIGS