IRC18_YEAST
ID IRC18_YEAST Reviewed; 224 AA.
AC P47056; D6VWE6;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Outer spore wall protein 6 {ECO:0000303|PubMed:23966878};
DE AltName: Full=Increased recombination centers protein 18 {ECO:0000303|PubMed:18085829};
DE Flags: Precursor;
GN Name=IRC18 {ECO:0000303|PubMed:18085829};
GN Synonyms=OSW6 {ECO:0000303|PubMed:23966878};
GN OrderedLocusNames=YJL037W {ECO:0000312|SGD:S000003574}; ORFNames=J1234;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP INDUCTION.
RX PubMed=11179416; DOI=10.1091/mbc.12.2.297;
RA Epstein C.B., Waddle J.A., Hale W. IV, Dave V., Thornton J., Macatee T.L.,
RA Garner H.R., Butow R.A.;
RT "Genome-wide responses to mitochondrial dysfunction.";
RL Mol. Biol. Cell 12:297-308(2001).
RN [5]
RP INDUCTION.
RX PubMed=14561723; DOI=10.1093/jb/mvg155;
RA Sakaki K., Tashiro K., Kuhara S., Mihara K.;
RT "Response of genes associated with mitochondrial function to mild heat
RT stress in yeast Saccharomyces cerevisiae.";
RL J. Biochem. 134:373-384(2003).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=18085829; DOI=10.1371/journal.pgen.0030228;
RA Alvaro D., Lisby M., Rothstein R.;
RT "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting
RT recombination.";
RL PLoS Genet. 3:E228-E228(2007).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23966878; DOI=10.1371/journal.pgen.1003700;
RA Lin C.P., Kim C., Smith S.O., Neiman A.M.;
RT "A highly redundant gene network controls assembly of the outer spore wall
RT in S. cerevisiae.";
RL PLoS Genet. 9:E1003700-E1003700(2013).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA He C., Jia C., Zhang Y., Xu P.;
RT "Enrichment-based proteogenomics identifies microproteins, missing
RT proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL J. Proteome Res. 17:2335-2344(2018).
CC -!- FUNCTION: Involved in spore wall assembly.
CC {ECO:0000269|PubMed:23966878}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: In respiratory-deficient cells and by heat.
CC {ECO:0000269|PubMed:11179416, ECO:0000269|PubMed:14561723}.
CC -!- DISRUPTION PHENOTYPE: Displays increased levels of spontaneous RAD52
CC foci in proliferating diploid cells (PubMed:18085829). A combined
CC deletion of the LOH1/OSW4 and IRC18/OSW6 has reduced dityrosine
CC incorporation in the outer spore wall (PubMed:23966878).
CC {ECO:0000269|PubMed:18085829, ECO:0000269|PubMed:23966878}.
CC -!- SIMILARITY: Belongs to the OSW4/6 family. {ECO:0000305}.
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DR EMBL; Z49312; CAA89328.1; -; Genomic_DNA.
DR EMBL; AY558244; AAS56570.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08762.1; -; Genomic_DNA.
DR PIR; S56809; S56809.
DR RefSeq; NP_012497.3; NM_001181471.3.
DR AlphaFoldDB; P47056; -.
DR SMR; P47056; -.
DR BioGRID; 33722; 20.
DR STRING; 4932.YJL037W; -.
DR iPTMnet; P47056; -.
DR PaxDb; P47056; -.
DR PRIDE; P47056; -.
DR EnsemblFungi; YJL037W_mRNA; YJL037W; YJL037W.
DR GeneID; 853414; -.
DR KEGG; sce:YJL037W; -.
DR SGD; S000003574; IRC18.
DR VEuPathDB; FungiDB:YJL037W; -.
DR GeneTree; ENSGT00940000182578; -.
DR HOGENOM; CLU_107727_0_0_1; -.
DR InParanoid; P47056; -.
DR OMA; GEXTEEQ; -.
DR BioCyc; YEAST:G3O-31503-MON; -.
DR PRO; PR:P47056; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47056; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030476; P:ascospore wall assembly; IGI:SGD.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Signal; Sporulation;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..224
FT /note="Outer spore wall protein 6"
FT /id="PRO_0000203069"
FT TOPO_DOM 27..174
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P47055"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P47055"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..224
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P47055"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 224 AA; 24746 MW; 6C17841176EADC1F CRC64;
MKVQMIERIF LIQLCLLTVV LASSRAVVEF ESTGTKLVNS LRVLAAYSQS SVCVDEKISG
IERQIEEVKD MYGNHSFILK GLNGILNNKV NMLTREIQME TVGNNTFETE TGKLTKGLNR
AVNISPFKYI KKFKTVSTKK FESLLNKYDL VAKKGGELTE EQKKKKEVLS RISRVVAATT
IEAGLAQGVV DLCITVTTSL CLVSASIGGV GFLIWLTIIY QALT
