ID   APRE_PSEAE              Reviewed;         432 AA.
AC   Q03025;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Alkaline protease secretion protein AprE;
GN   Name=aprE; OrderedLocusNames=PA1247;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=1427098; DOI=10.1016/0378-1119(92)90160-q;
RA   Duong F., Lazdunski A., Cami B., Murgier M.;
RT   "Sequence of a cluster of genes controlling synthesis and secretion of
RT   alkaline protease in Pseudomonas aeruginosa: relationships to other
RT   secretory pathways.";
RL   Gene 121:47-54(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Involved in the secretion of alkaline protease.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; X64558; CAA45856.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04636.1; -; Genomic_DNA.
DR   PIR; G83489; G83489.
DR   PIR; S26697; S26697.
DR   RefSeq; NP_249938.1; NC_002516.2.
DR   RefSeq; WP_003082540.1; NZ_QZGE01000005.1.
DR   AlphaFoldDB; Q03025; -.
DR   SMR; Q03025; -.
DR   STRING; 287.DR97_690; -.
DR   PaxDb; Q03025; -.
DR   PRIDE; Q03025; -.
DR   EnsemblBacteria; AAG04636; AAG04636; PA1247.
DR   GeneID; 881276; -.
DR   KEGG; pae:PA1247; -.
DR   PATRIC; fig|208964.12.peg.1294; -.
DR   PseudoCAP; PA1247; -.
DR   HOGENOM; CLU_023976_1_1_6; -.
DR   InParanoid; Q03025; -.
DR   OMA; WQSERDN; -.
DR   PhylomeDB; Q03025; -.
DR   BioCyc; PAER208964:G1FZ6-1272-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   GO; GO:0046903; P:secretion; IDA:PseudoCAP.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   InterPro; IPR010129; T1SS_HlyD.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   TIGRFAMs; TIGR01843; type_I_hlyD; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..432
FT                   /note="Alkaline protease secretion protein AprE"
FT                   /id="PRO_0000201862"
FT   TOPO_DOM        1..14
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        15..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..432
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        378..379
FT                   /note="AL -> RV (in Ref. 1; CAA45856)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   432 AA;  48053 MW;  4C845C65DECF15EE CRC64;
     MTRTVKRDEN AYARLGWLLV LFGFGGALLW AAFAPLDQGV AVPATVIISG QRKSVQHPLG
     GVVKHILVRD GQHVEAGEPL IRMEPTQARA NVDSLLNRYA NARLNQARLQ AEYDGRRTLE
     MPAGLAEQAP LPTLGERLEL QRQLLHSRQT ALANELSALR ANIEGLRAQL EGLRQTEGNQ
     RLQQRLLNSQ LSGARDLAEE GYMPRNQLLE QERQLAEVNA RLSESSGRFG QIRQSIAEAQ
     MRIAQREEEY RKEVNGQLAE TQVNARTLWE ELSSARYELR HAEIRAPVSG YVAGLKVFTD
     GGVIGPGELL MYIVPNSDSL EVEGQLAVNL VDRIHSGLPV EMLFTAFNQS KTPRVTGEVT
     MVSADRLLDE QNKQPYYALR AQVDAAAMGK LKGLQIRPGM AVQVFVRTGE RSLLNYLFKP
     LFDRAHVALA EN