IRC22_YEAST
ID IRC22_YEAST Reviewed; 225 AA.
AC P40006; D3DLP6; Q05243;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Increased recombination centers protein 22;
DE Flags: Precursor;
GN Name=IRC22; OrderedLocusNames=YEL001C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1293887; DOI=10.1002/yea.320081212;
RA te Heesen S., Aebi M.;
RT "Genetic and physical mapping of the WBP1 locus close to CENV.";
RL Yeast 8:1101-1103(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=18085829; DOI=10.1371/journal.pgen.0030228;
RA Alvaro D., Lisby M., Rothstein R.;
RT "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting
RT recombination.";
RL PLoS Genet. 3:E228-E228(2007).
CC -!- FUNCTION: Is probably involved in a pathway contributing to genomic
CC integrity.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:14562095}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:14562095}.
CC -!- DISRUPTION PHENOTYPE: Displays increased levels of spontaneous RAD52
CC foci in proliferating diploid cells. {ECO:0000269|PubMed:18085829}.
CC -!- MISCELLANEOUS: Present with 7110 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the IRC22 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA45869.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X64582; CAA45869.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18530; AAB64478.1; -; Genomic_DNA.
DR EMBL; AY692686; AAT92705.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07650.1; -; Genomic_DNA.
DR PIR; S50458; S50458.
DR RefSeq; NP_010915.1; NM_001178816.1.
DR AlphaFoldDB; P40006; -.
DR BioGRID; 36730; 50.
DR MINT; P40006; -.
DR STRING; 4932.YEL001C; -.
DR MaxQB; P40006; -.
DR PaxDb; P40006; -.
DR PRIDE; P40006; -.
DR EnsemblFungi; YEL001C_mRNA; YEL001C; YEL001C.
DR GeneID; 856717; -.
DR KEGG; sce:YEL001C; -.
DR SGD; S000000727; IRC22.
DR VEuPathDB; FungiDB:YEL001C; -.
DR eggNOG; ENOG502S3VP; Eukaryota.
DR HOGENOM; CLU_078554_1_0_1; -.
DR InParanoid; P40006; -.
DR OMA; YQEEWIP; -.
DR BioCyc; YEAST:G3O-30131-MON; -.
DR PRO; PR:P40006; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40006; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..225
FT /note="Increased recombination centers protein 22"
FT /id="PRO_0000014315"
FT TOPO_DOM 25..169
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 203..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..225
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 225 AA; 24992 MW; 94C23FF5C98B8C39 CRC64;
MRFSMLIGFN LLTALSSFCA AISANNSDNV EHEQEVAEAV APPSINIEVK YDVVGKESEN
HDSFLEFYAE DTATLAYNVT NWEDTNITIF GVNGTIVTYP HGYPVADITG ASIGPYEMEV
NGTSKFGQDV TLNLPEGQYF LIPFLLASRF DEIVRIAAPP TLFEIVSPPI SFFNPQFLSV
QVIFLAIIGG VSYYYMKSKT NQRPSKKSAT VKKVDESWLP ETYKK
