APRF1_ARATH
ID APRF1_ARATH Reviewed; 330 AA.
AC Q9LYK6;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Protein ANTHESIS POMOTING FACTOR 1 {ECO:0000303|PubMed:27968983};
GN Name=APRF1 {ECO:0000303|PubMed:27968983};
GN OrderedLocusNames=At5g14530 {ECO:0000312|Araport:AT5G14530};
GN ORFNames=T15N1.20 {ECO:0000312|EMBL:CAB87620.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=27968983; DOI=10.1016/j.plantsci.2016.09.015;
RA Kapolas G., Beris D., Katsareli E., Livanos P., Zografidis A., Roussis A.,
RA Milioni D., Haralampidis K.;
RT "APRF1 promotes flowering under long days in Arabidopsis thaliana.";
RL Plant Sci. 253:141-153(2016).
CC -!- FUNCTION: Component of a chromatin regulatory complex involved in
CC regulating chromatin structure in the nucleus (By similarity). Promotes
CC flowering under long days (LD) via the regulation of bolting
CC (PubMed:27968983). {ECO:0000250|UniProtKB:Q9VLN1,
CC ECO:0000269|PubMed:27968983}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27968983}.
CC -!- TISSUE SPECIFICITY: Expressed in the shoot apical meristem (SAM),
CC embryos, seedlings, cotyledons, leaves primordia, young leaves and
CC roots. {ECO:0000269|PubMed:27968983}.
CC -!- DEVELOPMENTAL STAGE: During seed development, accumulates in the
CC endosperm and in the developing embryo. Strongly expressed following
CC germination throughout seedling growth, especially in aerial part.
CC Present in cotyledons, including the vascular system, hydathodes and
CC trichomes, as well as in young leaves and leaves primordia. In roots,
CC mostly expressed 7 days after sowing. Localized in the central cylinder
CC of roots and the lower hypocotyl area. Observed in the shoot apical
CC meristem (SAM) and in the vascular system, particularly in the xylem
CC parenchyma and phloem. During reproductive development, observed in
CC flowers of stages 13-15, predominantly in the male reproductive
CC tissues, mostly in the developing pollen grains, the anther tissues and
CC the filaments, and, to a lower extent, in the vascular system of sepals
CC and the gynoecium, and in petals. After fertilization, confined to the
CC placenta and the abscission zone of the siliques.
CC {ECO:0000269|PubMed:27968983}.
CC -!- DISRUPTION PHENOTYPE: Altered shoot apical meristem (SAM) and increased
CC growth rates, as well as prolonged vegetative phase and delayed bolting
CC resulting in an impairment of flowering under long days (LD). Altered
CC expression of FLC and SOC1. {ECO:0000269|PubMed:27968983}.
CC -!- SIMILARITY: Belongs to the WD repeat SWD2 family. {ECO:0000305}.
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DR EMBL; AL163792; CAB87620.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92044.1; -; Genomic_DNA.
DR EMBL; BT025881; ABF85783.1; -; mRNA.
DR EMBL; AY086271; AAM64344.1; -; mRNA.
DR PIR; T48626; T48626.
DR RefSeq; NP_196957.1; NM_121457.3.
DR AlphaFoldDB; Q9LYK6; -.
DR SMR; Q9LYK6; -.
DR STRING; 3702.AT5G14530.1; -.
DR iPTMnet; Q9LYK6; -.
DR PaxDb; Q9LYK6; -.
DR PRIDE; Q9LYK6; -.
DR ProteomicsDB; 244441; -.
DR EnsemblPlants; AT5G14530.1; AT5G14530.1; AT5G14530.
DR GeneID; 831304; -.
DR Gramene; AT5G14530.1; AT5G14530.1; AT5G14530.
DR KEGG; ath:AT5G14530; -.
DR Araport; AT5G14530; -.
DR TAIR; locus:2222612; AT5G14530.
DR eggNOG; KOG1446; Eukaryota.
DR HOGENOM; CLU_044117_1_0_1; -.
DR InParanoid; Q9LYK6; -.
DR OMA; CVLNGDH; -.
DR OrthoDB; 1146727at2759; -.
DR PhylomeDB; Q9LYK6; -.
DR PRO; PR:Q9LYK6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LYK6; baseline and differential.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0048188; C:Set1C/COMPASS complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0080182; P:histone H3-K4 trimethylation; IBA:GO_Central.
DR GO; GO:0016571; P:histone methylation; IGI:TAIR.
DR GO; GO:0048586; P:regulation of long-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037867; Swd2/WDR82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19861; PTHR19861; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Flowering; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..330
FT /note="Protein ANTHESIS POMOTING FACTOR 1"
FT /id="PRO_0000439059"
FT REPEAT 22..61
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 112..151
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 153..191
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 198..237
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 242..281
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 284..323
FT /note="WD 6"
FT /evidence="ECO:0000255"
SQ SEQUENCE 330 AA; 36628 MW; 16C6E9C3127FC1C4 CRC64;
MSLTELDDGL VRRMAMGAVF SDFGGKIHSV GFHRTDDLLV TSSEDDSLRL FDIANAKQLK
ITYHKKHGTD RVCFTHHPSS LICSSRYNLE STGESLRYLS MYDNRILRYF KGHKDRVVSL
CMSPINDSFM SGSLDRSVRL WDLRVNACQG ILHLRGRPAV AYDQQGLVFA IAMEGGAVKL
FDSRCYDKGP FDTFLVGGDT AEVNDIKFSN DGKSMLLTTT NNNIYVLDAY RGEKKCGFSL
EPSQGTPIEA TFTPDGKYVL SGSGDGTLHA WNIENPSEVA RWENNIGVVS CLKWAPRRAM
FVAASTVLTF WIPNDGESPA PADPPTDQQQ
