IRC5_YEAST
ID IRC5_YEAST Reviewed; 853 AA.
AC P43610; D6VTS1;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Uncharacterized ATP-dependent helicase IRC5;
DE EC=3.6.4.-;
DE AltName: Full=Increased recombination centers protein 5;
GN Name=IRC5; OrderedLocusNames=YFR038W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8686381;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<177::aid-yea896>3.0.co;2-a;
RA Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M.,
RA Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H.,
RA Hanaoka F., Murakami Y.;
RT "Fifteen open reading frames in a 30.8 kb region of the right arm of
RT chromosome VI from Saccharomyces cerevisiae.";
RL Yeast 12:177-190(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP IDENTIFICATION OF FRAMESHIFT.
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA Gates K., Gaffney T.D., Philippsen P.;
RT "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT comparison to the genome of a related fungus: Ashbya gossypii.";
RL Genome Biol. 4:R45.1-R45.13(2003).
RN [5]
RP IDENTIFICATION OF FRAMESHIFT.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=18085829; DOI=10.1371/journal.pgen.0030228;
RA Alvaro D., Lisby M., Rothstein R.;
RT "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting
RT recombination.";
RL PLoS Genet. 3:E228-E228(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Is probably involved in a pathway contributing to genomic
CC integrity.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Displays increased levels of spontaneous RAD52
CC foci in proliferating diploid cells. {ECO:0000269|PubMed:18085829}.
CC -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA09277.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D50617; BAA09277.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006940; DAA12481.1; -; Genomic_DNA.
DR PIR; S56293; S56293.
DR RefSeq; NP_116696.2; NM_001180003.1.
DR AlphaFoldDB; P43610; -.
DR SMR; P43610; -.
DR BioGRID; 31196; 72.
DR DIP; DIP-5300N; -.
DR IntAct; P43610; 1.
DR MINT; P43610; -.
DR STRING; 4932.YFR038W; -.
DR iPTMnet; P43610; -.
DR MaxQB; P43610; -.
DR PaxDb; P43610; -.
DR PRIDE; P43610; -.
DR EnsemblFungi; YFR038W_mRNA; YFR038W; YFR038W.
DR GeneID; 850599; -.
DR KEGG; sce:YFR038W; -.
DR SGD; S000001934; IRC5.
DR VEuPathDB; FungiDB:YFR038W; -.
DR eggNOG; KOG0385; Eukaryota.
DR GeneTree; ENSGT00940000173484; -.
DR HOGENOM; CLU_000315_17_3_1; -.
DR InParanoid; P43610; -.
DR OMA; MNDRKYL; -.
DR BioCyc; YEAST:G3O-30485-MON; -.
DR PRO; PR:P43610; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43610; protein.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:SGD.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IBA:GO_Central.
DR GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0006312; P:mitotic recombination; IMP:SGD.
DR GO; GO:0044030; P:regulation of DNA methylation; IBA:GO_Central.
DR CDD; cd18009; DEXHc_HELLS_SMARCA6; 1.
DR Gene3D; 3.40.50.10810; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR044753; HELLS_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038718; SNF2-like_sf.
DR InterPro; IPR000330; SNF2_N.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00176; SNF2-rel_dom; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..853
FT /note="Uncharacterized ATP-dependent helicase IRC5"
FT /id="PRO_0000074385"
FT DOMAIN 234..401
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 607..758
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 43..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 548..580
FT /evidence="ECO:0000255"
FT MOTIF 352..355
FT /note="DEGH box"
FT BINDING 247..254
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 853 AA; 96966 MW; 91EF0E200197FF28 CRC64;
MSRCSNAALM TVVEDAVGAR VAARTRNMSN GVNYREKEVN DLTADISDSD SDLDSEDNKH
GKGDNDTAPI WLQDDVHSDE DIQLDSEDDS DTEAVQAQVV DKLAKDTKSE QKSLDDELSE
MDTKTVSLKL KKLNEFVRQS QVYSSIIADT LLHRSNEVAN ANTKDNSNSD DEEHSSKKRK
TKKKSITDFF KKQKKNEDTT TQNGAPDDAA IKQPRLLKNC ILKPYQLEGL NWLITLYENG
LNGILADEMG LGKTVQSIAL LAFIYEMDTK GPFLVTAPLS TLDNWMNEFA KFAPDLPVLK
YYGTNGYKER SAKLKNFFKQ HGGTGIVITS YEIILRDTDL IMSQNWKFLI VDEGHRLKNI
NCRLIKELKK INTSNRLLLT GTPLQNNLAE LWSLLNFIMP DIFADFEIFN KWFDFDSLNL
GSGSNSEALN KLINDELQKN LISNLHTILK PFLLRRLKKV VLANILPPKR EYIINCPMTS
AQEKFYKAGL NGKLKKTMFK ELIKDFFTLN DEYIGHVSNR SIRDFINYKL SGNETSNTDN
KINPTLLQMD KLYKKNLQME ISNKKLQNMM MQLRQIIDST FLFYFPYLHP EDLTLETLLK
TSGKLQILQK LIPPLISEGH KVLIYSQFVN MLDLIEDWCD LNSFATFRID GSVNNETRKD
QLEKFNSSKD KHNIFLLSTR AAGLGINLVG ADTVVLFDSD WNPQVDLQAM DRCHRIGQES
PVIVYRLCCD NTIEHVILTR AANKRNLERM VIQMGKFNNL KKLALNEGSF LKANKAGVNV
TNKDLVQELS MLLMSDESNI GFENGGQKEN KATEGQLTDK EVEELTNRSL EAYKANRVVD
LPHVKLFETT SGL
