ID   APRF_PSEAE              Reviewed;         481 AA.
AC   Q03027;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Alkaline protease secretion protein AprF;
GN   Name=aprF; OrderedLocusNames=PA1248;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=1427098; DOI=10.1016/0378-1119(92)90160-q;
RA   Duong F., Lazdunski A., Cami B., Murgier M.;
RT   "Sequence of a cluster of genes controlling synthesis and secretion of
RT   alkaline protease in Pseudomonas aeruginosa: relationships to other
RT   secretory pathways.";
RL   Gene 121:47-54(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Involved in the secretion of alkaline protease.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; X64558; CAA45857.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04637.1; -; Genomic_DNA.
DR   PIR; S26698; S26698.
DR   RefSeq; NP_249939.1; NC_002516.2.
DR   RefSeq; WP_003110827.1; NZ_QZGE01000005.1.
DR   AlphaFoldDB; Q03027; -.
DR   SMR; Q03027; -.
DR   STRING; 287.DR97_689; -.
DR   PaxDb; Q03027; -.
DR   PRIDE; Q03027; -.
DR   EnsemblBacteria; AAG04637; AAG04637; PA1248.
DR   GeneID; 881227; -.
DR   KEGG; pae:PA1248; -.
DR   PATRIC; fig|208964.12.peg.1295; -.
DR   PseudoCAP; PA1248; -.
DR   HOGENOM; CLU_012817_0_2_6; -.
DR   InParanoid; Q03027; -.
DR   OMA; LDCFESK; -.
DR   PhylomeDB; Q03027; -.
DR   BioCyc; PAER208964:G1FZ6-1273-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:1990281; C:efflux pump complex; IBA:GO_Central.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015288; F:porin activity; IBA:GO_Central.
DR   GO; GO:0046903; P:secretion; IDA:PseudoCAP.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010130; T1SS_OMP_TolC.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01844; type_I_sec_TolC; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   CHAIN           1..481
FT                   /note="Alkaline protease secretion protein AprF"
FT                   /id="PRO_0000196246"
FT   REGION          462..481
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   481 AA;  54471 MW;  514F4133946CDB70 CRC64;
     MTMRRLMTWL FGAFLLLLRE DAFALGLLDG YHLALENDPQ FQAAIQEHEA GRQYRALGRA
     ALLPRLVYSY NRGRSWSDVT QTTTRGDFKE DRDYDSYVST LSLQQPLFDY EAFSRYRKGV
     AQALLSDERF RSQSQELLVR VLEAYTGALL AQDQIELARA QKRSYREQFQ LNQRQFERGN
     GTRTDTLETQ ARFNLAQAQE IEARDSQDAA LRELERLVGA PLEIADLAPL GERFQVRPLS
     PASYTAWRDL ALAENPELAS LRHAVDVARY EVEQNRADFL PRLGLYASTG KSKSGSENTY
     NQRYETDSVG IQLSVPLFSG GETLAATRQA THRMEKSHYD LDDKVRETLN QVRKMYNQSS
     SSAAKIRAYE MTVDSARTLV MATRKSIAAG VRVNLDLLNA EQALYSAMNE LSKAKYDYLT
     AWARLRFYAG VLDEADLELV AANFVSGETP ARRRDCATTD CPAPLHTLSK TDTEENRSAL
     N