IRC8_YEAST
ID IRC8_YEAST Reviewed; 822 AA.
AC P47046; D6VWD2;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Uncharacterized protein IRC8;
DE AltName: Full=Increased recombination centers protein 8;
GN Name=IRC8; OrderedLocusNames=YJL051W; ORFNames=J1156;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-690, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
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DR EMBL; Z49326; CAA89342.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08748.1; -; Genomic_DNA.
DR PIR; S56823; S56823.
DR RefSeq; NP_012484.1; NM_001181484.1.
DR AlphaFoldDB; P47046; -.
DR BioGRID; 33704; 112.
DR MINT; P47046; -.
DR STRING; 4932.YJL051W; -.
DR iPTMnet; P47046; -.
DR PaxDb; P47046; -.
DR PRIDE; P47046; -.
DR EnsemblFungi; YJL051W_mRNA; YJL051W; YJL051W.
DR GeneID; 853396; -.
DR KEGG; sce:YJL051W; -.
DR SGD; S000003587; IRC8.
DR VEuPathDB; FungiDB:YJL051W; -.
DR eggNOG; ENOG502RMJZ; Eukaryota.
DR HOGENOM; CLU_019067_0_0_1; -.
DR InParanoid; P47046; -.
DR OMA; SHTIDSP; -.
DR BioCyc; YEAST:G3O-31515-MON; -.
DR PRO; PR:P47046; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47046; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005933; C:cellular bud; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006312; P:mitotic recombination; IMP:SGD.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..822
FT /note="Uncharacterized protein IRC8"
FT /id="PRO_0000203064"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..44
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..120
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..822
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 271..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 698..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 751..783
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 751..777
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 690
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 822 AA; 91256 MW; BE850C5B409F8820 CRC64;
MCHNSVRSGN KAGFLGIKFG SALLSIATGA IAIALLCKFH DHEAVLIVIV CSTLLYGIPS
LISFITETVF APSKFHIGYF YNVLNFALPL ITMGCTVDYF HNTLRSPISV QSESHRVYIT
TLDSLLIFTL FINGIQLGFF LKDGNANNFG SSSNNISTDQ YDKEANAVEN GRFVPLKNSS
QTLTPDLELL HGSPKSMNGV AWLINELSTN SNTNANKTIS SDENSNSSVI RHKLGPISTS
KCPKKPSHSH FSKLKKYNSF FLGPKENRYK RNTQQATKVP TEKKSNHRSS QYVSRLSTIS
DISKSFLNFL ALNEKNGNST STARTPSEGR VSIIINEGNN TLKYKTPHDS HTIDSPNLEL
EREAIGRINS ALLPACLRVT DKMISPQQST QNEDSYQATP LIPQVEVDDD FYVGDILMTN
ELQDIPQVPR ISSDIEDDFE QQYTKHVDLP ARVTLEMWEK DQEKILQKVT TNRDKSKLLP
PFRFTSESDM DPSTSTELEV ELHAQNNFSF PFKSAGLQIA TSDQFNQQEF KTSDTISELD
EYLHDPSIQE EDASQLIESS LNQNNLSSTT IDNGPKDMSR FSTRHSPTKS IISMISGSGS
VKHQHSHSTL SNFFTGHSRN NSQINQLLQG SSSNMMSNTS PHSSPTKSLR MRFGKKLSLS
NISDTMSPYE GSTTDPINYS FGHGHNKNQS IDFSYVRTLQ SSHSPTKSTS GNSRRDSLNN
DRTQSTVNER ALRTASTLFY LQHNNATCTL NGEEPVLDTP QSIQSSSSGS EQESAGSGSG
YPEVVFSEYD REKWNVLRNL KEIAPEKTIE SGPVEELVSP SK
