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IRE1_CAEEL
ID   IRE1_CAEEL              Reviewed;         967 AA.
AC   Q09499; Q8WRG0;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 2.
DT   03-AUG-2022, entry version 171.
DE   RecName: Full=Serine/threonine-protein kinase/endoribonuclease ire-1;
DE   AltName: Full=Inositol-requiring protein 2;
DE   Includes:
DE     RecName: Full=Serine/threonine-protein kinase;
DE              EC=2.7.11.1;
DE   Includes:
DE     RecName: Full=Endoribonuclease;
DE              EC=3.1.26.-;
DE   Flags: Precursor;
GN   Name=ire-1 {ECO:0000312|WormBase:C41C4.4a};
GN   ORFNames=C41C4.4 {ECO:0000312|WormBase:C41C4.4a};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=11779465; DOI=10.1016/s0092-8674(01)00612-2;
RA   Shen X., Ellis R.E., Lee K., Liu C.-Y., Yang K., Solomon A., Yoshida H.,
RA   Morimoto R., Kurnit D.M., Mori K., Kaufman R.J.;
RT   "Complementary signaling pathways regulate the unfolded protein response
RT   and are required for C. elegans development.";
RL   Cell 107:893-903(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16184190; DOI=10.1371/journal.pgen.0010037;
RA   Shen X., Ellis R.E., Sakaki K., Kaufman R.J.;
RT   "Genetic interactions due to constitutive and inducible gene regulation
RT   mediated by the unfolded protein response in C. elegans.";
RL   PLoS Genet. 1:e37-e37(2005).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF GLY-723.
RX   PubMed=18216284; DOI=10.1091/mbc.e07-06-0547;
RA   Uccelletti D., Pascoli A., Farina F., Alberti A., Mancini P.,
RA   Hirschberg C.B., Palleschi C.;
RT   "APY-1, a novel Caenorhabditis elegans apyrase involved in unfolded protein
RT   response signalling and stress responses.";
RL   Mol. Biol. Cell 19:1337-1345(2008).
RN   [5]
RP   FUNCTION, AND MUTAGENESIS OF GLY-723.
RX   PubMed=20733002; DOI=10.1128/mcb.00922-10;
RA   Mao X.R., Crowder C.M.;
RT   "Protein misfolding induces hypoxic preconditioning via a subset of the
RT   unfolded protein response machinery.";
RL   Mol. Cell. Biol. 30:5033-5042(2010).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23149939; DOI=10.1128/mcb.01298-12;
RA   Shiimori M., Inoue K., Sakamoto H.;
RT   "A specific set of exon junction complex subunits is required for the
RT   nuclear retention of unspliced RNAs in Caenorhabditis elegans.";
RL   Mol. Cell. Biol. 33:444-456(2013).
CC   -!- FUNCTION: Senses unfolded proteins in the lumen of the endoplasmic
CC       reticulum via its N-terminal domain which leads to enzyme auto-
CC       activation (PubMed:11779465). The active endoribonuclease domain
CC       splices xbp-1 precursor mRNA to produce the mature form which then
CC       induces transcription of UPR target genes (PubMed:11779465). Unfolded
CC       protein response (UPR) transcriptional activation by ire-1, as well as
CC       translational attenuation by pek-1 in a complementary pathway,
CC       maintains ER homeostasis (PubMed:11779465, PubMed:16184190). Regulates
CC       the transcriptional up-regulation of nucleoside-diphosphatase apy-1 and
CC       many other genes, upon ER stress (PubMed:18216284, PubMed:16184190). By
CC       activating the UPR pathway during non-lethal hypoxia pre-conditioning,
CC       confers adaptive protection to subsequent exposure to hypoxia
CC       (PubMed:20733002). ire-1 and pek-1 are redundant genes that control a
CC       pathway essential for larval development and survival (PubMed:11779465,
CC       PubMed:16184190). Plays a role in the nuclear retention of unspliced
CC       mRNAs (PubMed:23149939). {ECO:0000269|PubMed:11779465,
CC       ECO:0000269|PubMed:16184190, ECO:0000269|PubMed:18216284,
CC       ECO:0000269|PubMed:20733002, ECO:0000269|PubMed:23149939}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: The kinase domain is activated by trans-
CC       autophosphorylation. Kinase activity is required for activation of the
CC       endoribonuclease domain (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:11779465}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:11779465}.
CC   -!- PTM: Autophosphorylated mainly on serine residues. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: In combination with RNAi-mediated knockdown of
CC       atf-6, causes sluggish movement, arrested development at the L2 larval
CC       stage, and lethality soon thereafter; larvae have intestinal
CC       degeneration and develop many vacuoles in the intestinal cells
CC       (PubMed:16184190). In combination with RNAi-mediated knockdown of pek-
CC       1, larvae have intestinal degeneration and develop many vacuoles in the
CC       intestinal cells (PubMed:16184190). RNAi-mediated knockdown restores
CC       fertility, reduces the accumulation of unspliced tra-2 in the cytoplasm
CC       and suppresses germ line masculinization caused by RNAi-mediated
CC       knockdown of rnp-4/RBM8A (PubMed:23149939).
CC       {ECO:0000269|PubMed:16184190, ECO:0000269|PubMed:23149939}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR   EMBL; AF435952; AAL30828.1; -; mRNA.
DR   EMBL; BX284602; CAA88100.2; -; Genomic_DNA.
DR   PIR; T19874; T19874.
DR   RefSeq; NP_001254135.1; NM_001267206.1.
DR   AlphaFoldDB; Q09499; -.
DR   SMR; Q09499; -.
DR   BioGRID; 39634; 3.
DR   STRING; 6239.C41C4.4a; -.
DR   iPTMnet; Q09499; -.
DR   EPD; Q09499; -.
DR   PaxDb; Q09499; -.
DR   PeptideAtlas; Q09499; -.
DR   PRIDE; Q09499; -.
DR   EnsemblMetazoa; C41C4.4a.1; C41C4.4a.1; WBGene00002147.
DR   GeneID; 174305; -.
DR   KEGG; cel:CELE_C41C4.4; -.
DR   UCSC; C41C4.4; c. elegans.
DR   CTD; 174305; -.
DR   WormBase; C41C4.4a; CE35836; WBGene00002147; ire-1.
DR   eggNOG; KOG1027; Eukaryota.
DR   GeneTree; ENSGT00940000167966; -.
DR   HOGENOM; CLU_004875_1_1_1; -.
DR   InParanoid; Q09499; -.
DR   OMA; HYLPDPR; -.
DR   OrthoDB; 1019877at2759; -.
DR   PhylomeDB; Q09499; -.
DR   Reactome; R-CEL-381070; IRE1alpha activates chaperones.
DR   PRO; PR:Q09499; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00002147; Expressed in germ line (C elegans) and 4 other tissues.
DR   ExpressionAtlas; Q09499; baseline and differential.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:WormBase.
DR   GO; GO:1990604; C:IRE1-TRAF2-ASK1 complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004521; F:endoribonuclease activity; ISS:WormBase.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:WormBase.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:WormBase.
DR   GO; GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IBA:GO_Central.
DR   GO; GO:0036498; P:IRE1-mediated unfolded protein response; IBA:GO_Central.
DR   GO; GO:0098787; P:mRNA cleavage involved in mRNA processing; IGI:UniProtKB.
DR   GO; GO:0002119; P:nematode larval development; IGI:WormBase.
DR   GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; ISS:WormBase.
DR   GO; GO:1990440; P:positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; IMP:UniProtKB.
DR   GO; GO:0006990; P:positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response; IMP:WormBase.
DR   GO; GO:0006468; P:protein phosphorylation; ISS:WormBase.
DR   GO; GO:0001666; P:response to hypoxia; IMP:UniProtKB.
DR   GO; GO:0035966; P:response to topologically incorrect protein; IMP:WormBase.
DR   GO; GO:1904576; P:response to tunicamycin; IMP:UniProtKB.
DR   Gene3D; 1.20.1440.180; -; 1.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR045133; IRE1/2-like.
DR   InterPro; IPR033523; IRE2.
DR   InterPro; IPR010513; KEN_dom.
DR   InterPro; IPR038357; KEN_sf.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR   InterPro; IPR002372; PQQ_repeat.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR13954; PTHR13954; 1.
DR   PANTHER; PTHR13954:SF15; PTHR13954:SF15; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   Pfam; PF01011; PQQ; 2.
DR   Pfam; PF06479; Ribonuc_2-5A; 1.
DR   SMART; SM00564; PQQ; 5.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS51392; KEN; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Endoplasmic reticulum; Glycoprotein; Hydrolase; Kinase;
KW   Membrane; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Serine/threonine-protein kinase; Signal;
KW   Stress response; Transferase; Transmembrane; Transmembrane helix;
KW   Unfolded protein response.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..967
FT                   /note="Serine/threonine-protein kinase/endoribonuclease
FT                   ire-1"
FT                   /id="PRO_0000024390"
FT   TOPO_DOM        22..438
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        439..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        456..967
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          518..778
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   DOMAIN          781..909
FT                   /note="KEN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00725"
FT   REGION          474..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          948..967
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        636
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         524..532
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         546
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   MOD_RES         672
FT                   /note="Phosphoserine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        100
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        188
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         723
FT                   /note="G->R: In zc14; loss of adaptive protection conferred
FT                   by non-lethal hypoxia treatment. Prevents transcriptional
FT                   up-regulation of apy-1 upon ER stress. Reduced UDPase
FT                   activity upon ER stress."
FT                   /evidence="ECO:0000269|PubMed:18216284,
FT                   ECO:0000269|PubMed:20733002"
SQ   SEQUENCE   967 AA;  109822 MW;  94947E3DED9C8687 CRC64;
     MRATFHLFTF IFLLLFSSVI CISTPGFRND HESIGDDEEK TSSTILVSTI DGRLRALDSE
     TGEIKWTLQE EPVLRSPSAV KQGFTFLPNP LDGSLYVLKN SSLKKLPFNI PQLVHASPCK
     GNDGILYAGS KKDVWFGIDP KTGLKVETLS SASADRICPA NQKQTIFLGR TEYRVSMFDE
     KNRGKTWNAT FNDYSAHLLP EVNTWPFKHY ASSSHGYILT FDRETGEMRW EQDLKQPVVA
     LYLLRDDGLH KLPFEVMGKE TMENVAKNIF TVDQWPTVLG VNAADPQTTS LTNQFFPALF
     VGESSFGLYA IEALVDHQTI TYSPKLLGPP LLEGPAPIAL TEMEKEEYLP PRRPIIRNIP
     PSITHKTSDG EYLLLGYHDR PMMTMATIIP TRYPVPGPHK AIGSTIERPP PQLLGPVEPQ
     KHEDTSFILL LLNNHPIPFY ATLVTMFALL LTVIWQCGRQ WDQQKSTSRM DSFEIVNNPG
     ESRSAQTSKQ SNRGSFGWAN RKIEIPEGWM AVGSKLMYSP SDILGTGCEG TVVYRGTFDG
     REVAVKRVVS EFVKFAHREA DLLRESDTHP HVIRYFCMES DSQFRYLALE LCIASLNDYV
     EQKEVQQNVT IALRDIMKQA TDGLAHLHAS KIVHRDMKPQ NVLITMASQR GEMRAVISDF
     GLCKRVQPGK NSISRGIASG LAGTDGWIAP EVLISASTSY PVDIFSLGCI FYYVLTSGTH
     PFGKSLHRQA NIVNGEYTLN KLADLDDWSL ADDLISSMLN VEPLHRLTAD AVLNHPFFWT
     SEKRLAYFSD VSDRVEKEED NSPVVRRIET DARIVVCGGW REKICDALKE DLRKFRTYKS
     FSVRDLLRAM RNKKHHYREL PEDVRQSLGD IPDQFLHYFT SRFPRLLLHV YKATEYCSGE
     AVFKRYYSDD VRARMYPIVE EEERVRKKIK EEMANEVWAR APKPVEQRTP LKLDKRNIKK
     KSNPNTD
 
 
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