IRE4_ARATH
ID IRE4_ARATH Reviewed; 1042 AA.
AC F4HPN2; Q0WLU7; Q9MAJ4;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Probable serine/threonine protein kinase IRE4 {ECO:0000305};
DE Short=AtIRE4 {ECO:0000303|PubMed:17237187};
DE EC=2.7.11.1 {ECO:0000305};
GN Name=IRE4 {ECO:0000303|PubMed:17237187};
GN OrderedLocusNames=At1g45160 {ECO:0000312|Araport:AT1G45160};
GN ORFNames=F27F5.23 {ECO:0000312|EMBL:AAF69167.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=17237187; DOI=10.1104/pp.106.092494;
RA Pislariu C.I., Dickstein R.;
RT "An IRE-like AGC kinase gene, MtIRE, has unique expression in the invasion
RT zone of developing root nodules in Medicago truncatula.";
RL Plant Physiol. 144:682-694(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000305};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=F4HPN2-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr
CC protein kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF69167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007915; AAF69167.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32085.1; -; Genomic_DNA.
DR EMBL; AK230091; BAF01910.1; -; mRNA.
DR PIR; H96509; H96509.
DR RefSeq; NP_175130.2; NM_103590.3. [F4HPN2-1]
DR AlphaFoldDB; F4HPN2; -.
DR SMR; F4HPN2; -.
DR STRING; 3702.AT1G45160.2; -.
DR iPTMnet; F4HPN2; -.
DR PaxDb; F4HPN2; -.
DR PRIDE; F4HPN2; -.
DR EnsemblPlants; AT1G45160.1; AT1G45160.1; AT1G45160. [F4HPN2-1]
DR GeneID; 841084; -.
DR Gramene; AT1G45160.1; AT1G45160.1; AT1G45160. [F4HPN2-1]
DR KEGG; ath:AT1G45160; -.
DR Araport; AT1G45160; -.
DR eggNOG; KOG0606; Eukaryota.
DR PRO; PR:F4HPN2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HPN2; baseline and differential.
DR Genevisible; F4HPN2; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Metal-binding;
KW Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.
FT CHAIN 1..1042
FT /note="Probable serine/threonine protein kinase IRE4"
FT /id="PRO_0000431356"
FT DOMAIN 670..955
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 956..1042
FT /note="AGC-kinase C-terminal"
FT /evidence="ECO:0000255"
FT ZN_FING 402..421
FT /note="C2H2-type; atypical"
FT /evidence="ECO:0000250|UniProtKB:Q9LE81"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 830..850
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 834..850
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 793
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 676..684
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 699
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 854
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P42818"
FT CONFLICT 313
FT /note="Y -> C (in Ref. 3; BAF01910)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1042 AA; 117997 MW; 2D7FA2E06004E77B CRC64;
MAEENRKDRG VSSTVAIPSG LNRIKTRLAS SGPRPEDSSD TVLKPPFNRN QKTIVPRGHG
RTTGSSKQER KGTKLSRWLA SYKPKYSCHP PKYACSSTTS SEEIKLRGKN SGKDEEKMIK
ISETNPPCSK SMGIKSFSHE LGPRGGVQTP YPRPHSYNDL KELLGSLHSR FDVAKETVDK
KLDVFVRDVK EAMEKMDPSC PEDREMAEQL LDVARACMEM TSAQLRATCE SIVQDLTRKR
KQCQAGLVKW LFSQLLFILT HCTRVVMFQK ETEPIDESSF RKFKECLERI PALETDWGST
PRVDDSGSGY PEYQRNEAGQ KFKRRDKESL ESETALDYVV PNDHGNNAAR EGYAAAKQEF
PSHEPQFDSK VVEQRFYLSD EYEDKMSNEP GKELGGSDYV ICRICEEEVP LFHLEPHSYI
CAYADKCEIN CVDVDERLLK LEEILEQIID SRSLNSFTQA GGLENSVLRK SGVASEGCSP
KINEWRNKGL EGMFEDLHEM DTAFIDESYT YPIHLKSHVG AKFCHHATSS STGSITSVSS
TNTPRTSHFD SYWLERHCPE QEDLRLMMDL SDIARCGAST DFSKEGSCDY IMACMQDIQA
VLKQGKLKAL VIDTFGGRIE KLLCEKYLHA RELTADKSSV GNIKESEDVL EHASATPQLL
LKDRISIDDF EIIKPISRGA FGKVFLARKR TTGDFFAIKV LKKLDMIRKN DIERILQERN
ILITVRYPFL VRFFYSFTCR DNLYLVMEYL NGGDLYSLLQ KVGCLDEEIA RIYIAELVLA
LEYLHSLKIV HRDLKPDNLL IAYNGHIKLT DFGLSKIGLI NNTIDLSGHE SDVSPRTNSH
HFQKNQEEER IRHSAVGTPD YLAPEILLGT EHGYAADWWS AGIVLFELLT GIPPFTASRP
EKIFDNILNG KMPWPDVPGE MSYEAQDLIN RLLVHEPEKR LGANGAAEVK SHPFFQGVDW
ENLALQKAAF VPQPESINDT SYFVSRFSES SCSDTETGNN SGSNPDSGDE VGIWKLHPFL
SRYSICNHRI YRKLFFLLLC VF
