ID   IRGA_VIBC3              Reviewed;         652 AA.
AC   A5F9G0; C3LX28; P27772; Q9KUP0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Iron-regulated outer membrane virulence protein;
DE   Flags: Precursor;
GN   Name=irgA; OrderedLocusNames=VC0395_A0028, VC395_0519;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1406279; DOI=10.1111/j.1365-2958.1992.tb01415.x;
RA   Goldberg M.B., Boyko S.A., Butterton J.R., Stoebner J.A., Payne S.M.,
RA   Calderwood S.B.;
RT   "Characterization of a Vibrio cholerae virulence factor homologous to the
RT   family of TonB-dependent proteins.";
RL   Mol. Microbiol. 6:2407-2418(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-151.
RX   PubMed=2174861; DOI=10.1128/jb.172.12.6863-6870.1990;
RA   Goldberg M.B., Boyko S.A., Calderwood S.B.;
RT   "Transcriptional regulation by iron of a Vibrio cholerae virulence gene and
RT   homology of the gene to the Escherichia coli fur system.";
RL   J. Bacteriol. 172:6863-6870(1990).
CC   -!- FUNCTION: Involved in the initial step of iron uptake by binding ferric
CC       vibriobactin, an iron chelatin siderophore that allows V.cholerae to
CC       extract iron from the environment.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- MISCELLANEOUS: Regulation of the irgA expression is negatively
CC       regulated at the transcriptional level by iron.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC       {ECO:0000305}.
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DR   EMBL; U72152; AAC44766.1; -; Genomic_DNA.
DR   EMBL; CP000627; ABQ21321.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08538.1; -; Genomic_DNA.
DR   PIR; S25265; S25265.
DR   RefSeq; WP_000086051.1; NZ_JAACZH010000029.1.
DR   AlphaFoldDB; A5F9G0; -.
DR   SMR; A5F9G0; -.
DR   STRING; 345073.VC395_0519; -.
DR   TCDB; 1.B.14.1.32; the outer membrane receptor (omr) family.
DR   EnsemblBacteria; ABQ21321; ABQ21321; VC0395_A0028.
DR   KEGG; vco:VC0395_A0028; -.
DR   KEGG; vcr:VC395_0519; -.
DR   PATRIC; fig|345073.21.peg.507; -.
DR   eggNOG; COG4771; Bacteria.
DR   HOGENOM; CLU_008287_18_2_6; -.
DR   OMA; PDKEKMM; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR   GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR   Gene3D; 2.170.130.10; -; 1.
DR   Gene3D; 2.40.170.20; -; 1.
DR   InterPro; IPR039426; BtuB-like.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR010916; TonB_box_CS.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   InterPro; IPR010917; TonB_rcpt_CS.
DR   PANTHER; PTHR30069; PTHR30069; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
DR   PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR   PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ion transport; Iron; Iron transport; Membrane;
KW   Receptor; Signal; TonB box; Transmembrane; Transmembrane beta strand;
KW   Transport; Virulence.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..652
FT                   /note="Iron-regulated outer membrane virulence protein"
FT                   /id="PRO_0000324812"
FT   MOTIF           33..40
FT                   /note="TonB box"
FT   MOTIF           635..652
FT                   /note="TonB C-terminal box"
SQ   SEQUENCE   652 AA;  71641 MW;  76789A0BEB6099C7 CRC64;
     MSRFNPSPVS LSVTLGLMFS ASAFAQDATK TDETMVVTAA GYAQVIQNAP ASISVISRED
     LESRYYRDVT DALKSVPGVT VTGGGDTTDI SIRGMGSNYT LILVDGKRQT SRQTRPNSDG
     PGIEQGWLPP LQAIERIEVI RGPMSTLYGS DAIGGVINII TRKDQQQWSG NVQLSTVVQE
     NRASGDEQSA NFFVTGPLSD ALSLQVYGQT TQRDEDEIEH GYGDKSLRSL TSKLNYQLNP
     DHQLQLEAGV SAQDRENNVG KSAQSSGCRG TCSNTDNQYR RNHVAVSHQG DWQGVGQSDT
     YLQYEENTNK SREMSIDNTV FKSTLVAPIG EHMLSFGVEG KHESLEDKTS NKISSRTHIS
     NTQWAGFIED EWALAEQFRL TFGGRLDHDK NYGSHFSPRV YGVWNLDPLW TVKGGVSTGF
     RAPQLREVTP DWGQVSGGGN IYGNPDLQPE TSINKELSLM YSTGSGLAAS LTAFHNDFKD
     KITRVACPAN ICTAGPNQWG ATPTYRVNID EAETYGAEAT LSLPITESVE LSSSYTYTHS
     EQKSGNFAGR PLLQLPKHLF NANLSWQTTD RLNSWANLNY RGKEMQPEGG ASNDDFIAPS
     YTFIDTGVTY ALTDTATIKA AVYNLFDQEV NYAEYGYVED GRRYWLGLDI AF