ID   IRGB_VIBC3              Reviewed;         298 AA.
AC   A5F9F9; C3LX27; P25543; Q9KUP1;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Iron-regulated virulence regulatory protein IrgB;
GN   Name=irgB; OrderedLocusNames=VC0395_A0027, VC395_0518;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1705025; DOI=10.1073/pnas.88.4.1125;
RA   Goldberg M.B., Boyko S.A., Calderwood S.B.;
RT   "Positive transcriptional regulation of an iron-regulated virulence gene in
RT   Vibrio cholerae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1125-1129(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Transcription activation of the irgA gene. In the presence of
CC       sufficient iron, transcription of both irgA and irgB is negatively
CC       regulated by a fur-like protein. In low iron conditions, negative
CC       regulation of transcription is removed, and production of IrgB leads to
CC       positive transcriptional activation of irgA.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; U72152; AAC44765.1; -; Genomic_DNA.
DR   EMBL; CP000627; ABQ21369.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08537.1; -; Genomic_DNA.
DR   PIR; A38579; A38579.
DR   RefSeq; WP_001153532.1; NZ_JAACZH010000029.1.
DR   AlphaFoldDB; A5F9F9; -.
DR   SMR; A5F9F9; -.
DR   STRING; 345073.VC395_0518; -.
DR   EnsemblBacteria; ABQ21369; ABQ21369; VC0395_A0027.
DR   KEGG; vco:VC0395_A0027; -.
DR   KEGG; vcr:VC395_0518; -.
DR   PATRIC; fig|345073.21.peg.506; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_16_2_6; -.
DR   OMA; YSDNIAM; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..298
FT                   /note="Iron-regulated virulence regulatory protein IrgB"
FT                   /id="PRO_0000321854"
FT   DOMAIN          1..59
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        19..38
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   298 AA;  34099 MW;  7548F821D131E56E CRC64;
     MQDLSAVKAF HALCQHKSLT AAAKALEQPK STLSRRLAQL EEDLGQSLLM RQGNRLTLTK
     AGEVFAVYSE QLLELANKSQ EALQELNNQV TGELTLVVHP NLIRGWLSQV LDEFMQQHST
     LKIRLLSQFQ HSDEVFEPDL IIWIEHAAPM GYRKERLGYW RYATYASPKY LAHRDKPTHP
     RELIHHPWID FIACRRAELE LHHPEFGSYS LPALESRLQS DNLAMQADAI AKGRGIGLLP
     TWFANGFETA HPGSLIPCVN GWQSQPTEIN CFYPLGRHPL RLRLFIDALR QARPDEWQ