ID   IRGB_VIBCH              Reviewed;         298 AA.
AC   P0C6D1; P25543; Q9KUP1;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Iron-regulated virulence regulatory protein IrgB;
GN   Name=irgB; OrderedLocusNames=VC_0474;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Transcription activation of the irgA gene. In the presence of
CC       sufficient iron, transcription of both irgA and irgB is negatively
CC       regulated by a fur-like protein. In low iron conditions, negative
CC       regulation of transcription is removed, and production of irgB leads to
CC       positive transcriptional activation of irgA (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE003852; AAF93647.1; -; Genomic_DNA.
DR   PIR; C82317; C82317.
DR   RefSeq; NP_230128.1; NC_002505.1.
DR   RefSeq; WP_001153529.1; NZ_LT906614.1.
DR   AlphaFoldDB; P0C6D1; -.
DR   SMR; P0C6D1; -.
DR   STRING; 243277.VC_0474; -.
DR   DNASU; 2615136; -.
DR   EnsemblBacteria; AAF93647; AAF93647; VC_0474.
DR   GeneID; 57739220; -.
DR   GeneID; 66938815; -.
DR   KEGG; vch:VC_0474; -.
DR   PATRIC; fig|243277.26.peg.448; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_16_2_6; -.
DR   OMA; YSDNIAM; -.
DR   BioCyc; VCHO:VC0474-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006351; P:transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..298
FT                   /note="Iron-regulated virulence regulatory protein IrgB"
FT                   /id="PRO_0000105659"
FT   DOMAIN          1..59
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        19..38
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   298 AA;  34069 MW;  6549F821C130E91F CRC64;
     MQDLSAVKAF HALCQHKSLT AAAKALEQPK STLSRRLAQL EEDLGQSLLM RQGNRLTLTK
     AGEVFAVYSE QLLELANKSQ EALQELNNQV TGELTLVVHP NLIRGWLSQV LDEFMQQHST
     LKIRLLSQFQ HSDEVFEPDL IIWIEHAAPM GYRKERLGYW RYAAYASPKY LAHRDKPTHP
     RELIHHPWID FIACRRAELE LHHPEFGSYS LPALESRLQS DNLAMQADAI AKGRGIGLLP
     TWFANGFETA HPGSLIPCVN GWQSQPTEIN CFYPLGRHPL RLRLFIDALR QARPDEWQ