IRO2_ORYSI
ID IRO2_ORYSI Reviewed; 248 AA.
AC A2WZ60;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Protein IRON-RELATED TRANSCRIPTION FACTOR 2 {ECO:0000305};
DE Short=OsIRO2 {ECO:0000305};
GN Name=IRO2 {ECO:0000305}; ORFNames=OsI_05230 {ECO:0000312|EMBL:EAY77256.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Transcription activator that binds to the DNA motif 5'-
CC CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as
CC well as of genes involved in iron homeostasis, thus contributing to
CC basal tolerance to iron deficiency, iron uptake from soil and iron
CC transport, particularly during seed maturation and germination.
CC Promotes the accumulation of mugineic acid family phytosiderophores
CC (MAs). Required for ethylene-mediated signaling during iron deficiency
CC responses. Improves growth and yield, especially in calcareous soil
CC with low iron availability. Promotes iron concentration in shoots and
CC grain. {ECO:0000250|UniProtKB:Q0JFZ0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
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DR EMBL; CM000126; EAY77256.1; -; Genomic_DNA.
DR AlphaFoldDB; A2WZ60; -.
DR SMR; A2WZ60; -.
DR STRING; 39946.A2WZ60; -.
DR EnsemblPlants; BGIOSGA000097-TA; BGIOSGA000097-PA; BGIOSGA000097.
DR Gramene; BGIOSGA000097-TA; BGIOSGA000097-PA; BGIOSGA000097.
DR HOGENOM; CLU_089779_0_0_1; -.
DR OMA; AIQISMR; -.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:1900706; P:positive regulation of siderophore biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0046685; P:response to arsenic-containing substance; IEA:EnsemblPlants.
DR GO; GO:0046686; P:response to cadmium ion; IEA:EnsemblPlants.
DR GO; GO:1990641; P:response to iron ion starvation; ISS:UniProtKB.
DR GO; GO:0071731; P:response to nitric oxide; IEA:EnsemblPlants.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 3: Inferred from homology;
KW Activator; DNA-binding; Ethylene signaling pathway; Iron; Nucleus;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..248
FT /note="Protein IRON-RELATED TRANSCRIPTION FACTOR 2"
FT /id="PRO_0000442064"
FT DOMAIN 68..120
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 68..81
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 82..120
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT MOTIF 68..75
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ SEQUENCE 248 AA; 27090 MW; 86B71AED6A6685D3 CRC64;
MEQLFVDDPA FASSMSSLEA DIFSGAGQLP SSPWLDLDLD DDVQDLSMAP TTANAVSSGY
GSGGSGSHRK LSHNAYERDR RKQLNELYSS LRALLPDADH TKKLSIPTTV SRVLKYIPEL
QKQVENLERK KKELTTTSTT NCKPGVLGSQ LMSEGMAPIV SATCINDMEI MVQVSLLSNV
AGSVLPLSKC IKVLENEGLH FISSSTSSGF GNRTFYSIHL QRSEGTINEE CPAFCERLEK
VVRNKAKL
