IRO3_ORYSJ
ID IRO3_ORYSJ Reviewed; 252 AA.
AC Q10KL8; Q8W397;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein IRON-RELATED TRANSCRIPTION FACTOR 3 {ECO:0000303|PubMed:20699001};
DE Short=OsIRO2 {ECO:0000303|PubMed:20699001};
DE AltName: Full=Basic helix-loop-helix protein 63 {ECO:0000303|PubMed:16896230};
DE Short=OsbHLH063 {ECO:0000303|PubMed:16896230};
DE AltName: Full=Transcription factor BHLH063 {ECO:0000305};
DE AltName: Full=bHLH transcription factor bHLH063 {ECO:0000305};
GN Name=IRO3 {ECO:0000303|PubMed:20699001};
GN Synonyms=BHLH063 {ECO:0000303|PubMed:16896230};
GN OrderedLocusNames=Os03g0379300 {ECO:0000312|EMBL:BAF12148.1},
GN LOC_Os03g26210 {ECO:0000312|EMBL:ABF96248.1};
GN ORFNames=OsJ_11051 {ECO:0000312|EMBL:EEE59148.1},
GN OSJNBa0013O08.3 {ECO:0000312|EMBL:AAL58252.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY IRON DEFICIENCY.
RX PubMed=20699001; DOI=10.1186/1471-2229-10-166;
RA Zheng L., Ying Y., Wang L., Wang F., Whelan J., Shou H.;
RT "Identification of a novel iron regulated basic helix-loop-helix protein
RT involved in Fe homeostasis in Oryza sativa.";
RL BMC Plant Biol. 10:166-166(2010).
CC -!- FUNCTION: Transcription factor that acts as negative regulator of the
CC iron deficiency response (PubMed:20699001). Suppresses the induction of
CC iron deficiency responsive genes, such as NAS1, NAS2, IRO2, IRT1,
CC YSL15, and NRAMP1 (PubMed:20699001). {ECO:0000269|PubMed:20699001}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:20699001}.
CC -!- INDUCTION: Strongly induced by iron deficiency, both in roots and
CC shoots. {ECO:0000269|PubMed:20699001}.
CC -!- MISCELLANEOUS: Plants overexpressing IRO3 are hypersensitive to iron
CC deficiency. {ECO:0000269|PubMed:20699001}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
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DR EMBL; AC084762; AAL58252.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96248.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96249.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12148.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE59148.1; -; Genomic_DNA.
DR EMBL; AK061515; BAG87978.1; -; mRNA.
DR EMBL; AK068704; BAG91038.1; -; mRNA.
DR EMBL; AP014959; BAS84435.1; -; Genomic_DNA.
DR RefSeq; XP_015633301.1; XM_015777815.1.
DR RefSeq; XP_015633302.1; XM_015777816.1.
DR AlphaFoldDB; Q10KL8; -.
DR SMR; Q10KL8; -.
DR STRING; 4530.OS03T0379300-01; -.
DR PaxDb; Q10KL8; -.
DR PRIDE; Q10KL8; -.
DR EnsemblPlants; Os03t0379300-01; Os03t0379300-01; Os03g0379300.
DR EnsemblPlants; Os03t0379300-02; Os03t0379300-02; Os03g0379300.
DR GeneID; 4332972; -.
DR Gramene; Os03t0379300-01; Os03t0379300-01; Os03g0379300.
DR Gramene; Os03t0379300-02; Os03t0379300-02; Os03g0379300.
DR KEGG; osa:4332972; -.
DR eggNOG; ENOG502RYQ6; Eukaryota.
DR HOGENOM; CLU_053417_1_0_1; -.
DR InParanoid; Q10KL8; -.
DR OMA; KPQPRYP; -.
DR OrthoDB; 1207684at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; DNA-binding; Nucleus; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..252
FT /note="Protein IRON-RELATED TRANSCRIPTION FACTOR 3"
FT /id="PRO_0000448627"
FT DOMAIN 36..86
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 36..49
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 50..86
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 135..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 76..131
FT /evidence="ECO:0000255"
FT COMPBIAS 150..164
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 252 AA; 27890 MW; 44F3B76EF3520BC1 CRC64;
MVPSERGDVA TAIRPAAADK LVHGPISDKK CRKKVPRKVH KSEREKLKRG HLNDLFGELG
NMLEADRQSN GKACILTDTT RILRDLLSQV KSLRQENSTL QNESNYVTME RNELQDENGA
LRSEISDLQN ELRMRATGSP GWGHGATGSP LPVPPSPGTV FPSQQPMQPS PMTTSTVFPL
QQPLPQPTVI EPSARQPLEL KLFLEAPPAE DPEPSEDQEA PNNVARPQPR YPTEASSWPI
SLGLPRMEDE QM
