ID   IROK_ECOLI              Reviewed;          21 AA.
AC   U3PVA8;
DT   11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT   11-DEC-2013, sequence version 1.
DT   03-AUG-2022, entry version 24.
DE   RecName: Full=Protein IroK;
DE   AltName: Full=3-hydroxypropionic acid resistance peptide;
GN   Name=iroK; OrderedLocusNames=b4706;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   IDENTIFICATION, AND FUNCTION.
RC   STRAIN=K12 / MG1655 / ATCC 29425;
RX   PubMed=22161628; DOI=10.1002/bit.24398;
RA   Warnecke T.E., Lynch M.D., Lipscomb M.L., Gill R.T.;
RT   "Identification of a 21 amino acid peptide conferring 3-hydroxypropionic
RT   acid stress-tolerance to Escherichia coli.";
RL   Biotechnol. Bioeng. 109:1347-1352(2012).
CC   -!- FUNCTION: Possible increased expression of this protein (due to
CC       mutations upstream of the start codon) is proposed to be responsible
CC       for resistance to 3-hydroxypropionic acid (3-HP).
CC       {ECO:0000269|PubMed:22161628}.
CC   -!- MISCELLANEOUS: Mutagenesis of the start codon from ATG to TTG, which
CC       should decrease protein expression, results in loss of 3-HP resistance.
CC       {ECO:0000305|PubMed:22161628}.
CC   -!- CAUTION: Encoded by the 21 C-terminal amino acids of the HcaR
CC       transcriptional activator, it is suggested to be produced by an
CC       independent promoter within the hcaR gene. However the peptide's
CC       existence has not been proven, nor has mutagenesis of HcaR itself
CC       rather than overexpression of IroK been ruled out as the cause of 3-HP
CC       resistance. {ECO:0000305}.
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DR   EMBL; U00096; AGW80435.1; -; Genomic_DNA.
DR   RefSeq; YP_008592385.1; NC_000913.3.
DR   AlphaFoldDB; U3PVA8; -.
DR   EnsemblBacteria; AGW80435; AGW80435; b4706.
DR   GeneID; 14678510; -.
DR   KEGG; eco:b4706; -.
DR   BioCyc; EcoCyc:MON0-4224; -.
DR   PRO; PR:U3PVA8; -.
DR   Proteomes; UP000000625; Chromosome.
PE   4: Predicted;
KW   Reference proteome.
FT   CHAIN           1..21
FT                   /note="Protein IroK"
FT                   /id="PRO_0000429438"
SQ   SEQUENCE   21 AA;  2330 MW;  DA2B4AC9F60D8E18 CRC64;
     MKPALRDFIA IVQERLASVT A