ID   IRS4_ASHGO              Reviewed;         744 AA.
AC   Q754G7;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Increased rDNA silencing protein 4;
GN   Name=IRS4; OrderedLocusNames=AFR103W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Positive regulator of phosphatidylinositol 4,5-bisphosphate
CC       turnover and negatively regulates signaling through the cell integrity
CC       pathway. Involved in rDNA silencing (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the IRS4 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53474.1; -; Genomic_DNA.
DR   RefSeq; NP_985650.1; NM_211004.1.
DR   AlphaFoldDB; Q754G7; -.
DR   STRING; 33169.AAS53474; -.
DR   EnsemblFungi; AAS53474; AAS53474; AGOS_AFR103W.
DR   GeneID; 4621893; -.
DR   KEGG; ago:AGOS_AFR103W; -.
DR   eggNOG; KOG0998; Eukaryota.
DR   HOGENOM; CLU_373362_0_0_1; -.
DR   InParanoid; Q754G7; -.
DR   OMA; KHHHRTR; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0016043; P:cellular component organization; IEA:UniProt.
DR   GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR   GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd00052; EH; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR000261; EH_dom.
DR   Pfam; PF12763; EF-hand_4; 1.
DR   SMART; SM00027; EH; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50031; EH; 1.
PE   3: Inferred from homology;
KW   Lipid metabolism; Reference proteome.
FT   CHAIN           1..744
FT                   /note="Increased rDNA silencing protein 4"
FT                   /id="PRO_0000308747"
FT   DOMAIN          617..706
FT                   /note="EH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT   REGION          1..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          76..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          187..237
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          260..282
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          302..350
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          366..412
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          522..574
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..229
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        260..277
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        398..412
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   744 AA;  82631 MW;  7D8E05484729D7D1 CRC64;
     MRFRSERQSK ILSASNGEAG EVHKASLKAA QATFKKHADG ARVNPRVPSA PGNSKGSMDG
     LVDPIAGLQL DVSGRRSKRG LVGRSLPPGG SANNINPRRI SSGVSCGEYD YKVAAAAAAR
     AARNENESIW PPTNAQHASP LLALPPLKLA GTHTRSSSNI SMSTLTSGNG SHLSLDEIIA
     TIKVNDPNSQ ISLKPPTSRR STQTGAYLSS RESPRGGVDN STESLPAGGS QYSPGRLHIP
     PIVINPALLG KDSQAILACS ESQASPSHCS ETTSPPPNTD GPVLPVRPPA VSSPIQSTYS
     FRSFSSNRMP RRIPPPCDPL SQLTKNEQKA VPQAAPLLES SEYSGGDSDY DGKQYSKACI
     SETDSALEDD DDFGSGYENF SNAEDYLSSQ DDFRERNDNN DNDDDDELDD DNLVESENDA
     TMEQDSQSDD DTESAKEFTV ANVSYSTLNK IPMNMTYRGT LPDLIPNYQR KQSKWMKLFK
     RHGQTGLGPS KNHPPTSHIY TQNDNALVQS KLHVRLKTTM RGILNDSSSS EPHQLSDDSE
     DSEEDYGSDR NSRSRSPGRR SGKKREKIRQ NLRYRNSFNE DKPWKSHLDV GYVTERERKR
     YEGMWVSNRD TYLDLLPWWN NGNYDETREV PEDGLMLNLV VTDIWSRSNL PQDTLAQIYD
     MVDTRRDGTL DRNSFVVGMW LVDQSLYGRK LPRELDRRVW DSVDKYVINV NGSNKNPKHH
     HRTRKKLLKK ELKLIKKESK SHNV