ID   IRS4_PICST              Reviewed;         628 AA.
AC   A3LPY4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Increased rDNA silencing protein 4;
GN   Name=IRS4; ORFNames=PICST_35192;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Positive regulator of phosphatidylinositol 4,5-bisphosphate
CC       turnover and negatively regulates signaling through the cell integrity
CC       pathway. Involved in rDNA silencing (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the IRS4 family. {ECO:0000305}.
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DR   EMBL; CP000496; ABN64577.2; -; Genomic_DNA.
DR   RefSeq; XP_001382606.2; XM_001382569.1.
DR   AlphaFoldDB; A3LPY4; -.
DR   SMR; A3LPY4; -.
DR   STRING; 4924.XP_001382606.2; -.
DR   EnsemblFungi; ABN64577; ABN64577; PICST_35192.
DR   GeneID; 4837446; -.
DR   KEGG; pic:PICST_35192; -.
DR   eggNOG; KOG0998; Eukaryota.
DR   HOGENOM; CLU_020874_0_0_1; -.
DR   InParanoid; A3LPY4; -.
DR   OMA; PEFLVGM; -.
DR   OrthoDB; 1070563at2759; -.
DR   Proteomes; UP000002258; Chromosome 2.
DR   GO; GO:0016043; P:cellular component organization; IEA:UniProt.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd00052; EH; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR000261; EH_dom.
DR   Pfam; PF12763; EF-hand_4; 1.
DR   SMART; SM00027; EH; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50031; EH; 1.
PE   3: Inferred from homology;
KW   Lipid metabolism; Reference proteome.
FT   CHAIN           1..628
FT                   /note="Increased rDNA silencing protein 4"
FT                   /id="PRO_0000308764"
FT   DOMAIN          527..616
FT                   /note="EH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT   REGION          21..83
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          107..138
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          253..276
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          297..380
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          398..421
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          497..527
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..80
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        116..138
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        300..319
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        339..365
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        497..515
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   628 AA;  69743 MW;  5DC1D053C4C60547 CRC64;
     MSENSTASHA AAMAAFKSLA NNNTSSSSIP SSYKIPTSAE SAKIRSPTPL NSNAAVPQNR
     PKRAATSVGV KSTTKPMAKS IATPISVKPS KANGFVSGVN KQRAPLPLAR ADSSLKSSRR
     YPQSKGLQAS SSSSTMPKDM INSVKQSIES KSVGKDPSQK RISSLYEPQE MLKNLRSSIS
     AKTVSGTSVR PMISEKNQAM INGIRDRIDS KRIVTDVSKT SSLSSEDVSH LQGHDLANTH
     TYQPLNNNDS FSSFGSSFSE VEPSSLSKAN GEEDEESIID NASATVQEHP PVNEEVAKNM
     NMDKTPKAKP KRRPPPDLVS GPREETSIPM FHDKSVESLP DGYSSTDADI TLENDRNSSQ
     SKFPQFPDIK KHKKTEKKGN FLKKYSRPLK CVDNEVNLLD SDEAEEEDET DSSYGKRAPQ
     ILTPPTQQVR LKSTMRKTNR RKEKKYMFNE NKPWKNHNEL SSISEQERKR YEGLWASNKG
     NYMNSVVTRL VGVDYDSGKS EPEVEEEEDS RKAARLSSKA GTFDSGKMSD QENFHGLLSV
     EVDQLIHGVV VRRIWKRSRL PRETLEAIWN LVDFRKDGTL NKPEFVVGMW LVDQCLYGRK
     LPKKVENEVW DSLGSIGLNV VVRKKGKR