IRT1_ORYSJ
ID IRT1_ORYSJ Reviewed; 374 AA.
AC Q75HB1; A0A0P0W166; Q7XJ41; Q8SBD0;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Fe(2+) transport protein 1;
DE AltName: Full=Fe(II) transport protein 1;
DE AltName: Full=Iron-regulated transporter 1;
DE Short=OsIRT1;
DE Flags: Precursor;
GN Name=IRT1; OrderedLocusNames=Os03g0667500, LOC_Os03g46470;
GN ORFNames=OSJNBa0056E06.2, OSJNBb0036M02.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC TISSUE=Root;
RX PubMed=12096107; DOI=10.1093/jxb/erf004;
RA Bughio N., Yamaguchi H., Nishizawa N.K., Nakanishi H., Mori S.;
RT "Cloning an iron-regulated metal transporter from rice.";
RL J. Exp. Bot. 53:1677-1682(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Quanhong Y., Rihe P., Aisheng X.;
RT "Isolation and characterization of a rice iron transporter gene.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16412081; DOI=10.1111/j.1365-313x.2005.02624.x;
RA Ishimaru Y., Suzuki M., Tsukamoto T., Suzuki K., Nakazono M., Kobayashi T.,
RA Wada Y., Watanabe S., Matsuhashi S., Takahashi M., Nakanishi H., Mori S.,
RA Nishizawa N.K.;
RT "Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.";
RL Plant J. 45:335-346(2006).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=19183299; DOI=10.1111/j.1365-3040.2009.01935.x;
RA Lee S., An G.;
RT "Over-expression of OsIRT1 leads to increased iron and zinc accumulations
RT in rice.";
RL Plant Cell Environ. 32:408-416(2009).
CC -!- FUNCTION: Iron transporter involved in the uptake of iron from the
CC rhizosphere across the plasma membrane in the root epidermal layer. May
CC also transport other divalent cations. {ECO:0000269|PubMed:16412081,
CC ECO:0000269|PubMed:19183299}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16412081};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16412081}.
CC -!- TISSUE SPECIFICITY: Expressed in companion cells in the upper region of
CC the root. {ECO:0000269|PubMed:16412081}.
CC -!- INDUCTION: By iron and zinc deficiency in roots.
CC {ECO:0000269|PubMed:12096107, ECO:0000269|PubMed:16412081,
CC ECO:0000269|PubMed:19183299}.
CC -!- MISCELLANEOUS: Plants overexpressing IRT1 show enhanced tolerance to
CC iron deficiency at the seedling stage, elevated content of cadmium,
CC iron and zinc in roots, shoots and mature seeds, and are sensitive to
CC an excess of zinc or cadmium.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC {ECO:0000305}.
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DR EMBL; AB070226; BAB85123.1; -; mRNA.
DR EMBL; AY327039; AAP92124.1; -; mRNA.
DR EMBL; AC135792; AAR87178.1; -; Genomic_DNA.
DR EMBL; AC145388; AAU89145.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98084.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12767.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS85654.1; -; Genomic_DNA.
DR EMBL; AK107681; BAG98121.1; -; mRNA.
DR RefSeq; XP_015632375.1; XM_015776889.1.
DR AlphaFoldDB; Q75HB1; -.
DR SMR; Q75HB1; -.
DR STRING; 4530.OS03T0667500-01; -.
DR PaxDb; Q75HB1; -.
DR PRIDE; Q75HB1; -.
DR EnsemblPlants; Os03t0667500-01; Os03t0667500-01; Os03g0667500.
DR GeneID; 4333669; -.
DR Gramene; Os03t0667500-01; Os03t0667500-01; Os03g0667500.
DR KEGG; osa:4333669; -.
DR eggNOG; KOG1558; Eukaryota.
DR HOGENOM; CLU_027089_3_0_1; -.
DR InParanoid; Q75HB1; -.
DR OMA; FGFAPLC; -.
DR OrthoDB; 981397at2759; -.
DR BioCyc; MetaCyc:MON-14000; -.
DR PlantReactome; R-OSA-9025727; Iron uptake and transport in root vascular system.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q75HB1; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015691; P:cadmium ion transport; IMP:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0006826; P:iron ion transport; IMP:UniProtKB.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IMP:UniProtKB.
DR InterPro; IPR003689; ZIP.
DR InterPro; IPR004698; Zn/Fe_permease_fun/pln.
DR Pfam; PF02535; Zip; 1.
DR TIGRFAMs; TIGR00820; zip; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..374
FT /note="Fe(2+) transport protein 1"
FT /id="PRO_0000398323"
FT TOPO_DOM 34..62
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..251
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..313
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 166..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 21
FT /note="L -> F (in Ref. 2; AAP92124)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="N -> K (in Ref. 2; AAP92124)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="L -> V (in Ref. 2; AAP92124)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 374 AA; 39057 MW; 4002BD6CDF85DD78 CRC64;
MATPRTLVPI LPPVAALLLL LVAASSIPIL AAAQPADACG GAPDQAAADG ACHDVPRALR
LKLIAIPTIL VSSVVGVCLP LLSRSVPALR PDGGLFAVVK AFASGVILAT GYMHVLPDAF
NNLTSPCLPR KPWSEFPFAA FVAMLAAVST LMADSLMLTY YNRSKPRPSS GGDVAAVADH
GESPDQGHRH GHGHGHGHGM AVAKPDDVEA TQVQLRRNRV VVQVLEIGIV VHSVVIGLGM
GASQNVCTIR PLVAAMCFHQ MFEGMGLGGC ILQAEYGRRM RSVLVFFFST TTPFGIALGL
ALTRVYRDNS PTALIVVGLL NAASAGLLHY MALVELLAAD FMGPKLQGNV RLQLAAFLAV
LLGAGGMSVM AKWA
