IRT3_ARATH
ID IRT3_ARATH Reviewed; 425 AA.
AC Q8LE59; Q8W244; Q9C957;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 3.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Fe(2+) transport protein 3, chloroplastic;
DE AltName: Full=Fe(II) transport protein 3;
DE AltName: Full=Iron-regulated transporter 3;
DE Flags: Precursor;
GN Name=IRT3; OrderedLocusNames=At1g60960; ORFNames=T7P1.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a role in the transport of iron in the plastids.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51647.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM62825.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF369915; AAL38438.1; -; Genomic_DNA.
DR EMBL; AC018908; AAG51647.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33753.1; -; Genomic_DNA.
DR EMBL; AY085604; AAM62825.1; ALT_INIT; mRNA.
DR PIR; B96635; B96635.
DR RefSeq; NP_564766.1; NM_104776.5.
DR AlphaFoldDB; Q8LE59; -.
DR STRING; 3702.AT1G60960.1; -.
DR PaxDb; Q8LE59; -.
DR PRIDE; Q8LE59; -.
DR ProteomicsDB; 247049; -.
DR EnsemblPlants; AT1G60960.1; AT1G60960.1; AT1G60960.
DR GeneID; 842387; -.
DR Gramene; AT1G60960.1; AT1G60960.1; AT1G60960.
DR KEGG; ath:AT1G60960; -.
DR Araport; AT1G60960; -.
DR TAIR; locus:2206011; AT1G60960.
DR eggNOG; KOG1558; Eukaryota.
DR HOGENOM; CLU_027089_3_0_1; -.
DR InParanoid; Q8LE59; -.
DR OrthoDB; 981397at2759; -.
DR PRO; PR:Q8LE59; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LE59; baseline and differential.
DR Genevisible; Q8LE59; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0006826; P:iron ion transport; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IMP:TAIR.
DR InterPro; IPR003689; ZIP.
DR InterPro; IPR004698; Zn/Fe_permease_fun/pln.
DR Pfam; PF02535; Zip; 1.
DR TIGRFAMs; TIGR00820; zip; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Ion transport; Iron; Iron transport; Membrane; Plastid;
KW Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..425
FT /note="Fe(2+) transport protein 3, chloroplastic"
FT /id="PRO_0000041638"
FT TOPO_DOM ?..64
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..137
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..301
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..369
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 391..404
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 21
FT /note="K -> Q (in Ref. 4; AAM62825)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="R -> L (in Ref. 4; AAM62825)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="G -> C (in Ref. 4; AAM62825)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 425 AA; 45089 MW; 0A1EC9D7041D4CC9 CRC64;
MFFVDVLWKL VPLYLFGSET KSLSATESIL QIVPEAMAAT SSNVLCNASE SDLCRDDSAA
FLLKFVAIAS ILLAGAAGVT IPLIGRNRRF LQTDGNLFVT AKAFAAGVIL ATGFVHMLAG
GTEALKNPCL PDFPWSKFPF PGFFAMIAAL ITLFVDFMGT QYYERKQERE ASESVEPFGR
EQSPGIVVPM IGEGTNDGKV FGEEDSGGIH IVGIHAHAAH HRHSHPPGHD SCEGHSKIDI
GHAHAHGHGH GHGHGHVHGG LDAVNGARHI VVSQVLELGI VSHSIIIGLS LGVSQSPCTI
RPLIAALSFH QFFEGFALGG CISQAQFRNK SATIMACFFA LTTPIGIGIG TAVASSFNSH
SVGALVTEGI LDSLSAGILV YMALVDLIAA DFLSTKMRCN FRLQIVSYVM LFLGAGLMSS
LAIWA
