APRR3_ARATH
ID APRR3_ARATH Reviewed; 495 AA.
AC Q9LVG4; C0SVU8; Q4PRK5; Q4PRK6;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Two-component response regulator-like APRR3;
DE AltName: Full=Pseudo-response regulator 3;
GN Name=APRR3; OrderedLocusNames=At5g60100; ORFNames=MGO3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=11100772; DOI=10.1093/pcp/pcd043;
RA Matsushika A., Makino S., Kojima M., Mizuno T.;
RT "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response
RT regulators in Arabidopsis thaliana: insight into the plant circadian
RT clock.";
RL Plant Cell Physiol. 41:1002-1012(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=cv. Cvi-0, and cv. Landsberg erecta;
RX PubMed=16461388; DOI=10.1104/pp.105.074518;
RA Darrah C., Taylor B.L., Edwards K.D., Brown P.E., Hall A., McWatters H.G.;
RT "Analysis of phase of LUCIFERASE expression reveals novel circadian
RT quantitative trait loci in Arabidopsis.";
RL Plant Physiol. 140:1464-1474(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=10945350; DOI=10.1093/pcp/41.6.791;
RA Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T.,
RA Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.;
RT "Genes encoding pseudo-response regulators: insight into His-to-Asp
RT phosphorelay and circadian rhythm in Arabidopsis thaliana.";
RL Plant Cell Physiol. 41:791-803(2000).
RN [7]
RP INDUCTION, AND PHOSPHORYLATION BY WNK1.
RX PubMed=12091722; DOI=10.1093/pcp/pcf084;
RA Murakami-Kojima M., Nakamichi N., Yamashino T., Mizuno T.;
RT "The APRR3 component of the clock-associated APRR1/TOC1 quintet is
RT phosphorylated by a novel protein kinase belonging to the WNK family, the
RT gene for which is also transcribed rhythmically in Arabidopsis thaliana.";
RL Plant Cell Physiol. 43:675-683(2002).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=14605371; DOI=10.1126/science.1082971;
RA Michael T.P., Salome P.A., Yu H.J., Spencer T.R., Sharp E.L., McPeek M.A.,
RA Alonso J.M., Ecker J.R., McClung C.R.;
RT "Enhanced fitness conferred by naturally occurring variation in the
RT circadian clock.";
RL Science 302:1049-1053(2003).
RN [9]
RP INTERACTION WITH APRR1.
RX PubMed=18055606; DOI=10.1105/tpc.107.054775;
RA Para A., Farre E.M., Imaizumi T., Pruneda-Paz J.L., Harmon F.G., Kay S.A.;
RT "PRR3 Is a vascular regulator of TOC1 stability in the Arabidopsis
RT circadian clock.";
RL Plant Cell 19:3462-3473(2007).
RN [10]
RP PHOSPHORYLATION, INDUCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP APRR1.
RX PubMed=18562312; DOI=10.1074/jbc.m803471200;
RA Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R.,
RA Somers D.E.;
RT "Post-translational regulation of the Arabidopsis circadian clock through
RT selective proteolysis and phosphorylation of pseudo-response regulator
RT proteins.";
RL J. Biol. Chem. 283:23073-23083(2008).
CC -!- FUNCTION: Controls photoperiodic flowering response. Component of the
CC circadian clock. Controls the degradation of APRR1/TOC1 by the SCF(ZTL)
CC complex. Expression of several members of the ARR-like family is
CC controlled by circadian rhythm. The particular coordinated sequential
CC expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian
CC waves that may be at the basis of the endogenous circadian clock.
CC {ECO:0000269|PubMed:11100772}.
CC -!- SUBUNIT: Interacts with APRR1/TOC1 (via N-terminus).
CC {ECO:0000269|PubMed:18055606, ECO:0000269|PubMed:18562312}.
CC -!- INTERACTION:
CC Q9LVG4; Q9LKL2: APRR1; NbExp=3; IntAct=EBI-1606968, EBI-618423;
CC Q9LVG4; C0SVM5: BBX19; NbExp=4; IntAct=EBI-1606968, EBI-4430993;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00357,
CC ECO:0000269|PubMed:18562312}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LVG4-1; Sequence=Displayed;
CC -!- INDUCTION: Expressed with a circadian rhythm showing a peak in the
CC evening. {ECO:0000269|PubMed:12091722, ECO:0000269|PubMed:16461388,
CC ECO:0000269|PubMed:18562312}.
CC -!- PTM: Phosphorylated by WNK1; during the night. Phosphorylation is
CC required for optimal interaction with APRR1/TOC1.
CC {ECO:0000269|PubMed:12091722, ECO:0000269|PubMed:18562312}.
CC -!- DISRUPTION PHENOTYPE: Shorter clock period but no effect on clock
CC phase. {ECO:0000269|PubMed:14605371}.
CC -!- SIMILARITY: Belongs to the ARR-like family. {ECO:0000305}.
CC -!- CAUTION: Lacks the phospho-accepting Asp (here Glu-116), present in the
CC receiver domain, which is one of the conserved features of two-
CC component response regulators (ARRs) family. {ECO:0000305}.
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DR EMBL; AB046956; BAB13744.1; -; mRNA.
DR EMBL; DQ060154; AAY62604.1; -; Genomic_DNA.
DR EMBL; DQ060155; AAY62605.1; -; Genomic_DNA.
DR EMBL; AB019231; BAA96939.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97276.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97277.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70160.1; -; Genomic_DNA.
DR EMBL; AB493801; BAH30639.1; -; mRNA.
DR RefSeq; NP_001190574.1; NM_001203645.2. [Q9LVG4-1]
DR RefSeq; NP_001331791.1; NM_001345387.1. [Q9LVG4-1]
DR RefSeq; NP_568919.1; NM_125403.3. [Q9LVG4-1]
DR AlphaFoldDB; Q9LVG4; -.
DR SMR; Q9LVG4; -.
DR BioGRID; 21376; 8.
DR IntAct; Q9LVG4; 5.
DR STRING; 3702.AT5G60100.2; -.
DR PRIDE; Q9LVG4; -.
DR EnsemblPlants; AT5G60100.1; AT5G60100.1; AT5G60100. [Q9LVG4-1]
DR EnsemblPlants; AT5G60100.3; AT5G60100.3; AT5G60100. [Q9LVG4-1]
DR EnsemblPlants; AT5G60100.5; AT5G60100.5; AT5G60100. [Q9LVG4-1]
DR GeneID; 836132; -.
DR Gramene; AT5G60100.1; AT5G60100.1; AT5G60100. [Q9LVG4-1]
DR Gramene; AT5G60100.3; AT5G60100.3; AT5G60100. [Q9LVG4-1]
DR Gramene; AT5G60100.5; AT5G60100.5; AT5G60100. [Q9LVG4-1]
DR KEGG; ath:AT5G60100; -.
DR Araport; AT5G60100; -.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_041215_0_0_1; -.
DR InParanoid; Q9LVG4; -.
DR OMA; EHAVMED; -.
DR PhylomeDB; Q9LVG4; -.
DR PRO; PR:Q9LVG4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVG4; baseline and differential.
DR Genevisible; Q9LVG4; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR InterPro; IPR045279; ARR-like.
DR InterPro; IPR010402; CCT_domain.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR43874; PTHR43874; 3.
DR Pfam; PF06203; CCT; 1.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51017; CCT; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Biological rhythms; Coiled coil; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..495
FT /note="Two-component response regulator-like APRR3"
FT /id="PRO_0000081436"
FT DOMAIN 65..183
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 442..484
FT /note="CCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00357"
FT REGION 188..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 465..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 349..372
FT /evidence="ECO:0000255"
FT COMPBIAS 223..270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..356
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..377
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 381..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..439
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..495
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 90
FT /note="V -> I (in strain: cv. Cvi-0)"
FT VARIANT 210
FT /note="Q -> E (in strain: cv. Cvi-0 and cv. Landsberg
FT erecta)"
FT VARIANT 267..268
FT /note="DA -> ES (in strain: cv. Cvi-0 and cv. Landsberg
FT erecta)"
FT VARIANT 329
FT /note="N -> K (in strain: cv. Cvi-0 and cv. Landsberg
FT erecta)"
SQ SEQUENCE 495 AA; 55286 MW; 933B89129FA1974F CRC64;
MCFNNIETGD EVETERQVFG SSEEDEFRVE DTARNTNNVQ ISQQQQQPLA HVVKWERYLP
VRSLKVLLVE NDDSTRHIVT ALLKNCSYEV TAVPDVLEAW RILEDEKSCI DLVLTEVDMP
VHSGTGLLSK IMSHKTLKNI PVIMMSSHDS MVLVFKCLSN GAVDFLVKPI RKNELKNLWQ
HVWRRCHSSS GSGSESGIHD KKSVKPESTQ GSENDASISD EHRNESGSSG GLSNQDGGSD
NGSGTQSSWT KRASDTKSTS PSNQFPDAPN KKGTYENGCA HVNRLKEAED QKEQIGTGSQ
TGMSMSKKAE EPGDLEKNAK YSVQALERNN DDTLNRSSGN SQVESKAPSS NREDLQSLEQ
TLKKTREDRD YKVGDRSVLR HSNLSAFSKY NNGATSAKKA PEENVESCSP HDSPIAKLLG
SSSSSDNPLK QQSSGSDRWA QREAALMKFR LKRKERCFEK KVRYHSRKKL AEQRPHVKGQ
FIRKRDDHKS GSEDN
