ID   IRX15_ARATH             Reviewed;         322 AA.
AC   Q9SNE5; A0MF18; Q56WV6; Q8LB40;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Protein IRREGULAR XYLEM 15;
DE            Short=AtIRX15;
GN   Name=IRX15; OrderedLocusNames=At3g50220; ORFNames=F11C160;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16969662; DOI=10.1007/s00438-006-0157-1;
RA   Ko J.H., Beers E.P., Han K.H.;
RT   "Global comparative transcriptome analysis identifies gene network
RT   regulating secondary xylem development in Arabidopsis thaliana.";
RL   Mol. Genet. Genomics 276:517-531(2006).
RN   [7]
RP   FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=21288268; DOI=10.1111/j.1365-313x.2010.04475.x;
RA   Jensen J.K., Kim H., Cocuron J.C., Orler R., Ralph J., Wilkerson C.G.;
RT   "The DUF579 domain containing proteins IRX15 and IRX15-L affect xylan
RT   synthesis in Arabidopsis.";
RL   Plant J. 66:387-400(2011).
RN   [8]
RP   FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE,
RP   AND SUBCELLULAR LOCATION.
RX   PubMed=21251108; DOI=10.1111/j.1365-313x.2011.04501.x;
RA   Brown D., Wightman R., Zhang Z., Gomez L.D., Atanassov I., Bukowski J.P.,
RA   Tryfona T., McQueen-Mason S.J., Dupree P., Turner S.;
RT   "Arabidopsis genes IRREGULAR XYLEM (IRX15) and IRX15L encode DUF579-
RT   containing proteins that are essential for normal xylan deposition in the
RT   secondary cell wall.";
RL   Plant J. 66:401-413(2011).
CC   -!- FUNCTION: Required for xylan biosynthesis, but not directly involved in
CC       catalyzing the addition of sugars to the growing polymer.
CC       {ECO:0000269|PubMed:21251108, ECO:0000269|PubMed:21288268}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass membrane protein {ECO:0000305}. Note=Also located in an unknown
CC       intracellular compartment that may have some role in the transport of
CC       cell wall polysaccharides into the cell wall.
CC       {ECO:0000269|PubMed:21251108, ECO:0000269|PubMed:21288268}.
CC   -!- TISSUE SPECIFICITY: Expressed in rosette leaves, stems and siliques.
CC       Expressed in the xylem. {ECO:0000269|PubMed:21251108,
CC       ECO:0000269|PubMed:21288268}.
CC   -!- DEVELOPMENTAL STAGE: Up-regulated during secondary cell wall
CC       deposition. {ECO:0000269|PubMed:16969662, ECO:0000269|PubMed:21251108}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype; due to the redundancy with
CC       IRX15-L. Irx15 and irx15-l double mutants have a mild collapsed xylem
CC       phenotype, irregular secondary cell wall margins in fiber cells, uneven
CC       distribution of xylan in the cell wall and a lower degree of xylan
CC       polymerization, but no visible growth phenotype.
CC       {ECO:0000269|PubMed:21251108, ECO:0000269|PubMed:21288268}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK28594.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL132976; CAB62301.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE78642.1; -; Genomic_DNA.
DR   EMBL; AK221924; BAD94344.1; -; mRNA.
DR   EMBL; DQ446752; ABE66005.1; -; mRNA.
DR   EMBL; DQ653142; ABK28594.1; ALT_SEQ; mRNA.
DR   EMBL; AY087437; AAM64983.1; -; mRNA.
DR   PIR; T45568; T45568.
DR   RefSeq; NP_190591.1; NM_114882.2.
DR   AlphaFoldDB; Q9SNE5; -.
DR   BioGRID; 9502; 9.
DR   IntAct; Q9SNE5; 9.
DR   STRING; 3702.AT3G50220.1; -.
DR   PaxDb; Q9SNE5; -.
DR   PRIDE; Q9SNE5; -.
DR   ProteomicsDB; 247052; -.
DR   EnsemblPlants; AT3G50220.1; AT3G50220.1; AT3G50220.
DR   GeneID; 824184; -.
DR   Gramene; AT3G50220.1; AT3G50220.1; AT3G50220.
DR   KEGG; ath:AT3G50220; -.
DR   Araport; AT3G50220; -.
DR   TAIR; locus:2074815; AT3G50220.
DR   eggNOG; ENOG502QRED; Eukaryota.
DR   HOGENOM; CLU_053427_2_0_1; -.
DR   InParanoid; Q9SNE5; -.
DR   OMA; RRCCAWL; -.
DR   OrthoDB; 1108083at2759; -.
DR   PhylomeDB; Q9SNE5; -.
DR   PRO; PR:Q9SNE5; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9SNE5; baseline and differential.
DR   Genevisible; Q9SNE5; AT.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR   GO; GO:0045492; P:xylan biosynthetic process; IMP:TAIR.
DR   InterPro; IPR006514; IRX15/IRX15L/IGXM.
DR   InterPro; IPR021148; Polysacc_synth_dom.
DR   PANTHER; PTHR31444; PTHR31444; 1.
DR   Pfam; PF04669; Polysacc_synt_4; 1.
DR   TIGRFAMs; TIGR01627; A_thal_3515; 1.
PE   2: Evidence at transcript level;
KW   Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..322
FT                   /note="Protein IRREGULAR XYLEM 15"
FT                   /id="PRO_0000420833"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        148
FT                   /note="V -> L (in Ref. 5; AAM64983)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        259
FT                   /note="T -> N (in Ref. 5; AAM64983)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        301
FT                   /note="K -> E (in Ref. 3; BAD94344)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   322 AA;  35807 MW;  14C39A59FB951CE2 CRC64;
     MKNGSGNTNT KLILLHPYIQ KQTSTTRLWL LAFVSFFTIV FLLTLLYTRD TIPSKNTSVA
     AAVAAVVTGG STPSASSPIS NSNLPTSAIN ALLHYASRSN DSFHMSYGEM KSISDVLRRC
     APPCNLLVFG LTHETLLWKS LNHNGRTVFI EENRYYAAYF EEIHPEIDVF DVQYTTKAHE
     AGELVTAAKE AAGNECRPVQ NLLFSDCKLG LNDLPNHVYD VDWDVIFVDG PRGDAHEGPG
     RMSSIFTAAV LARSKKGGTP KTHVFVHDYY RDVERLCGDE FLCRENLVES NDLLAHYVLD
     KMDKNSTKFC NGRKKRSVSS LS