IRX2_HUMAN
ID IRX2_HUMAN Reviewed; 471 AA.
AC Q9BZI1; Q68A19; Q7Z2I7;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Iroquois-class homeodomain protein IRX-2;
DE AltName: Full=Homeodomain protein IRXA2;
DE AltName: Full=Iroquois homeobox protein 2;
GN Name=IRX2; Synonyms=IRXA2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Hansen L., Wu Q., Tommerup N.;
RT "Characterization of the human homeobox two-cluster Iroquois gene family.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RA Sugiyama A., Inoue H., Oka M.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 101-181.
RX PubMed=11435706; DOI=10.1159/000056921;
RA Ogura K., Matsumoto K., Kuroiwa A., Isobe T., Otoguro T., Jurecic V.,
RA Baldini A., Matsuda Y., Ogura T.;
RT "Cloning and chromosome mapping of human and chicken Iroquois (IRX)
RT genes.";
RL Cytogenet. Cell Genet. 92:320-325(2001).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
CC -!- INTERACTION:
CC Q9BZI1; Q99471: PFDN5; NbExp=3; IntAct=EBI-12166369, EBI-357275;
CC Q9BZI1; Q9UPG8: PLAGL2; NbExp=3; IntAct=EBI-12166369, EBI-2876622;
CC Q9BZI1; Q08117-2: TLE5; NbExp=3; IntAct=EBI-12166369, EBI-11741437;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000305}.
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DR EMBL; AY335939; AAQ16545.1; -; Genomic_DNA.
DR EMBL; AY335940; AAQ16546.1; -; mRNA.
DR EMBL; AB188492; BAD37140.1; -; mRNA.
DR EMBL; BC065189; AAH65189.1; -; mRNA.
DR EMBL; AF319967; AAK01202.1; -; Genomic_DNA.
DR CCDS; CCDS3868.1; -.
DR RefSeq; NP_001127694.1; NM_001134222.1.
DR RefSeq; NP_150366.1; NM_033267.4.
DR RefSeq; XP_011512281.1; XM_011513979.2.
DR AlphaFoldDB; Q9BZI1; -.
DR SMR; Q9BZI1; -.
DR BioGRID; 127504; 6.
DR IntAct; Q9BZI1; 3.
DR STRING; 9606.ENSP00000372056; -.
DR iPTMnet; Q9BZI1; -.
DR PhosphoSitePlus; Q9BZI1; -.
DR BioMuta; IRX2; -.
DR DMDM; 47117908; -.
DR EPD; Q9BZI1; -.
DR jPOST; Q9BZI1; -.
DR MassIVE; Q9BZI1; -.
DR MaxQB; Q9BZI1; -.
DR PaxDb; Q9BZI1; -.
DR PeptideAtlas; Q9BZI1; -.
DR PRIDE; Q9BZI1; -.
DR ProteomicsDB; 79848; -.
DR Antibodypedia; 9121; 188 antibodies from 27 providers.
DR DNASU; 153572; -.
DR Ensembl; ENST00000302057.6; ENSP00000307006.5; ENSG00000170561.13.
DR Ensembl; ENST00000382611.10; ENSP00000372056.6; ENSG00000170561.13.
DR GeneID; 153572; -.
DR KEGG; hsa:153572; -.
DR MANE-Select; ENST00000302057.6; ENSP00000307006.5; NM_033267.5; NP_150366.1.
DR UCSC; uc003jda.4; human.
DR CTD; 153572; -.
DR DisGeNET; 153572; -.
DR GeneCards; IRX2; -.
DR HGNC; HGNC:14359; IRX2.
DR HPA; ENSG00000170561; Tissue enhanced (brain, breast, salivary gland, skin).
DR MalaCards; IRX2; -.
DR MIM; 606198; gene.
DR neXtProt; NX_Q9BZI1; -.
DR OpenTargets; ENSG00000170561; -.
DR Orphanet; 457246; Clear cell sarcoma of kidney.
DR PharmGKB; PA29924; -.
DR VEuPathDB; HostDB:ENSG00000170561; -.
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000161198; -.
DR HOGENOM; CLU_048118_1_0_1; -.
DR InParanoid; Q9BZI1; -.
DR OMA; HPHEDAS; -.
DR OrthoDB; 768315at2759; -.
DR PhylomeDB; Q9BZI1; -.
DR TreeFam; TF319371; -.
DR PathwayCommons; Q9BZI1; -.
DR SignaLink; Q9BZI1; -.
DR SIGNOR; Q9BZI1; -.
DR BioGRID-ORCS; 153572; 11 hits in 1090 CRISPR screens.
DR GeneWiki; IRX2; -.
DR GenomeRNAi; 153572; -.
DR Pharos; Q9BZI1; Tbio.
DR PRO; PR:Q9BZI1; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q9BZI1; protein.
DR Bgee; ENSG00000170561; Expressed in upper arm skin and 120 other tissues.
DR Genevisible; Q9BZI1; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0048468; P:cell development; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0072272; P:proximal/distal pattern formation involved in metanephric nephron development; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0072086; P:specification of loop of Henle identity; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR003893; Iroquois_homeo.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00548; IRO; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..471
FT /note="Iroquois-class homeodomain protein IRX-2"
FT /id="PRO_0000049153"
FT DNA_BIND 112..175
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 176..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..244
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 186
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
SQ SEQUENCE 471 AA; 49129 MW; 3B370608B5223C3C CRC64;
MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG SAAFTAQAAT
GFGSPLQYSA DAAAAAAGFP SYMGAPYDAH TTGMTGAISY HPYGSAAYPY QLNDPAYRKN
ATRDATATLK AWLNEHRKNP YPTKGEKIML AIITKMTLTQ VSTWFANARR RLKKENKMTW
APRNKSEDED EDEGDATRSK DESPDKAQEG TETSAEDEGI SLHVDSLTDH SCSAESDGEK
LPCRAGDPLC ESGSECKDKY DDLEDDEDDD EEGERGLAPP KPVTSSPLTG LEAPLLSPPP
EAAPRGGRKT PQGSRTSPGA PPPASKPKLW SLAEIATSDL KQPSLGPGCG PPGLPAAAAP
ASTGAPPGGS PYPASPLLGR PLYYTSPFYG NYTNYGNLNA ALQGQGLLRY NSAAAAPGEA
LHTAPKAASD AGKAGAHPLE SHYRSPGGGY EPKKDASEGC TVVGGGVQPY L
