IRX2_MOUSE
ID IRX2_MOUSE Reviewed; 474 AA.
AC P81066; O55121; Q3UFG3; Q9ERN1; Q9JLL4;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2002, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Iroquois-class homeodomain protein IRX-2;
DE AltName: Full=Homeodomain protein IRXA2;
DE AltName: Full=Iroquois homeobox protein 2;
DE AltName: Full=Iroquois-class homeobox protein Irx6;
GN Name=Irx2; Synonyms=Irx6, Irxa2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=FVB/N; TISSUE=Embryonic heart;
RX PubMed=10926765; DOI=10.1006/dbio.2000.9801;
RA Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W.,
RA Moorman A.F.M.;
RT "Patterning the embryonic heart: identification of five mouse Iroquois
RT homeobox genes in the developing heart.";
RL Dev. Biol. 224:263-274(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Brain;
RX PubMed=10704856; DOI=10.1016/s0925-4773(99)00263-4;
RA Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.;
RT "Expression of two novel mouse Iroquois-class homeobox genes during
RT neurogenesis.";
RL Mech. Dev. 91:317-321(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 109-186, AND DEVELOPMENTAL STAGE.
RX PubMed=9486539; DOI=10.1016/s0925-4773(97)00165-2;
RA Bosse A., Zulch A., Becker M.B., Torres M., Gomez-Skarmeta J.-L.,
RA Modolell J., Gruss P.;
RT "Identification of the vertebrate Iroquois homeobox gene family with
RT overlapping expression during early development of the nervous system.";
RL Mech. Dev. 69:169-181(1997).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=17875669; DOI=10.1101/gad.450707;
RA Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.;
RT "The prepattern transcription factor Irx3 directs nephron segment
RT identity.";
RL Genes Dev. 21:2358-2370(2007).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in specific and overlapping patterns with
CC Irx1 and Irx3 in the developing and adult metanephric kidney. In the
CC adult metanephros, renal expression is found in the loop of Henle in
CC the S3 proximal tubule segment and in the thick ascending limb (TAL) of
CC the distal tubule. {ECO:0000269|PubMed:17875669}.
CC -!- DEVELOPMENTAL STAGE: First expressed at 8.0 dpc. During neural tube
CC closure (8.5 dpc), expression appears for the first time in the
CC rhombencephalon in the presumptive region of future rhombomere 4.
CC During neurogenesis (9.5 dpc to 10.5 dpc), predominantly expressed
CC along the anteroposterior axis of the CNS in the mesencephalon,
CC metencephalon and rhombencephalon. Expression is strong in the tectum
CC of the mesencephalon and in the hindbrain, expression is restricted to
CC rhombomeres. Expression in the spinal cord is weak and confined to the
CC alar plate. Beginning at 9.5 dpc, expressed in the epithelial component
CC of the branchial arches and foregut. At 10.5 dpc, expression extends
CC rostrally into the dorsal diencephalon. Starting at the otic vesicle
CC stage, shows regionalized expression in the developing inner ear with
CC expression in the entire otic vesicle from 10.5 dpc onwards. From 10.5
CC dpc onwards, weak expression begins in the limb bud. Also expressed in
CC other tissues during organogenesis; at 9.5 dpc, expressed in the
CC superficial ectoderm surrounding the body and in the region of the
CC foregut, which will form the pharynx and the lung bud. at 10.5 dpc,
CC found in the cephalic mesenchyme around the optic vesicle. By 12.5 dpc,
CC still expressed in the mesenchyme, and expression begins in specific
CC subsets of post-mitotic cells in the neuroretina. As development
CC ensues, expression increases in the neuroretina and mesenchymal
CC expression gradually decreases. At 16.5 dpc, expressed exclusively in
CC the inner neuroblast layers of the neuroretina. Expressed in the
CC developing heart in the ventricular septum from the onset of its
CC formation (10.5 dpc) onward. In fetal stages, expression becomes
CC confined to the myocardium of the atrioventricular bundle and bundle
CC branches of the forming ventricular conduction system.
CC {ECO:0000269|PubMed:10704856, ECO:0000269|PubMed:10926765,
CC ECO:0000269|PubMed:9486539}.
CC -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000305}.
CC -!- CAUTION: Called Irx6 by PubMed:10704856. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF63956.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF295369; AAG10083.1; -; mRNA.
DR EMBL; AF165986; AAF63956.1; ALT_FRAME; mRNA.
DR EMBL; AK148520; BAE28598.1; -; mRNA.
DR EMBL; BC029750; AAH29750.1; -; mRNA.
DR EMBL; Y15000; CAA75232.1; ALT_SEQ; mRNA.
DR CCDS; CCDS26628.1; -.
DR RefSeq; NP_034704.1; NM_010574.3.
DR AlphaFoldDB; P81066; -.
DR SMR; P81066; -.
DR STRING; 10090.ENSMUSP00000073976; -.
DR iPTMnet; P81066; -.
DR PhosphoSitePlus; P81066; -.
DR PaxDb; P81066; -.
DR PRIDE; P81066; -.
DR ProteomicsDB; 269335; -.
DR Antibodypedia; 9121; 188 antibodies from 27 providers.
DR DNASU; 16372; -.
DR Ensembl; ENSMUST00000074372; ENSMUSP00000073976; ENSMUSG00000001504.
DR GeneID; 16372; -.
DR KEGG; mmu:16372; -.
DR UCSC; uc007rde.1; mouse.
DR CTD; 153572; -.
DR MGI; MGI:1197526; Irx2.
DR VEuPathDB; HostDB:ENSMUSG00000001504; -.
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000161198; -.
DR HOGENOM; CLU_048118_1_0_1; -.
DR InParanoid; P81066; -.
DR OMA; HPHEDAS; -.
DR OrthoDB; 768315at2759; -.
DR PhylomeDB; P81066; -.
DR TreeFam; TF319371; -.
DR BioGRID-ORCS; 16372; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Irx2; mouse.
DR PRO; PR:P81066; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P81066; protein.
DR Bgee; ENSMUSG00000001504; Expressed in pretectal region and 245 other tissues.
DR ExpressionAtlas; P81066; baseline and differential.
DR Genevisible; P81066; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0048468; P:cell development; IBA:GO_Central.
DR GO; GO:0001656; P:metanephros development; IEP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0072272; P:proximal/distal pattern formation involved in metanephric nephron development; IEP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0072086; P:specification of loop of Henle identity; IEP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR003893; Iroquois_homeo.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00548; IRO; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..474
FT /note="Iroquois-class homeodomain protein IRX-2"
FT /id="PRO_0000049154"
FT DNA_BIND 115..177
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 177..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 262..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZI1"
FT CONFLICT 27..33
FT /note="ALAAPRS -> GGARA (in Ref. 2; AAF63956)"
FT /evidence="ECO:0000305"
FT CONFLICT 185..186
FT /note="NK -> EQ (in Ref. 5)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 474 AA; 49484 MW; 04E39C42E717F98B CRC64;
MSYPQGYLYQ APGSLALYSC PAYGASALAA PRSEELARSA SGSAFSPYPG SAAFTAQAAT
GFGSPLQYSA DAAAAAAAGF PSYVGSPYDT HTTGMTGAIS YHPYGSAAYP YQLNDPAYRK
NATRDATATL KAWLNEHRKN PYPTKGEKIM LAIITKMTLT QVSTWFANAR RRLKKENKMT
WAPRNKSEDE DEDEGDASRS KEESSDKAQD GTETSAEDEG ISLHVDSLTD HSCSAESDGE
KLPCRAGDAL CESGSECKDK FEDLEDEEDE EDECERDLAP PKPVTSSPLT GVEAPLLSPA
PEAAPRGGSG GKTPLGSRTS PGAPPPASKP KLWSLAEIAT SDLKQPSLGP GCGPPGLPAA
AAPASTGAPP GGSPYSASPL LGRHLYYTSP FYGNYTNYGN LNAALQGQGL LRYNTAASSP
GETLHAMPKA ASDTGKAGSH SLESHYRPPG GGYEPKKDTS EGCAVVGAGV QTYL
