IRX2_XENTR
ID IRX2_XENTR Reviewed; 456 AA.
AC Q66IK1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Iroquois-class homeodomain protein irx-2 {ECO:0000250|UniProtKB:Q9BZI1};
DE AltName: Full=Iroquois homeobox protein 2 {ECO:0000250|UniProtKB:Q9BZI1};
GN Name=irx2 {ECO:0000312|EMBL:AAH81317.1};
GN Synonyms=iro2 {ECO:0000250|UniProtKB:Q6DCQ1};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000312|EMBL:AAH81317.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAH81317.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19268445; DOI=10.1016/j.ydbio.2009.02.028;
RA Rodriguez-Seguel E., Alarcon P., Gomez-Skarmeta J.L.;
RT "The Xenopus Irx genes are essential for neural patterning and define the
RT border between prethalamus and thalamus through mutual antagonism with the
RT anterior repressors Fezf and Arx.";
RL Dev. Biol. 329:258-268(2009).
CC -!- FUNCTION: Acts partially redundantly with other irx members in neural
CC patterning. Required for formation of the posterior forebrain,
CC midbrain, hindbrain, and to a lesser extent, spinal cord. Acts early in
CC neural plate development to induce expression of some but not all
CC proneural genes, and specify a neural precursor state. Also up-
CC regulates repressors that prevent neuronal differentiation. Patterns
CC the neuroectoderm in both the anterior/posterior and dorsal/ventral
CC axes. Probably dispensable for pronephric kidney development.
CC {ECO:0000250|UniProtKB:Q6DCQ1, ECO:0000269|PubMed:19268445}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the neural plate in overlapping
CC patterns with other irx members, which all share an anterior border of
CC expression. Also expressed in the placodes. Broadly expressed in the
CC tailbud rhombencephalon (hindbrain). Outside the nervous system and at
CC tailbud stages, expressed in the developing otic vesicle, branchial
CC arches, prospective heart region and pronephros.
CC {ECO:0000269|PubMed:19268445}.
CC -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000255}.
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DR EMBL; BC081317; AAH81317.1; -; mRNA.
DR RefSeq; NP_001008113.1; NM_001008112.1.
DR AlphaFoldDB; Q66IK1; -.
DR SMR; Q66IK1; -.
DR STRING; 8364.ENSXETP00000027698; -.
DR PaxDb; Q66IK1; -.
DR DNASU; 493475; -.
DR Ensembl; ENSXETT00000027698; ENSXETP00000027698; ENSXETG00000012653.
DR GeneID; 493475; -.
DR KEGG; xtr:493475; -.
DR CTD; 153572; -.
DR Xenbase; XB-GENE-480618; irx2.
DR eggNOG; KOG0773; Eukaryota.
DR HOGENOM; CLU_048118_1_0_1; -.
DR InParanoid; Q66IK1; -.
DR OMA; NTHACSY; -.
DR OrthoDB; 768315at2759; -.
DR PhylomeDB; Q66IK1; -.
DR TreeFam; TF319371; -.
DR Proteomes; UP000008143; Chromosome 6.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000012653; Expressed in heart and 13 other tissues.
DR ExpressionAtlas; Q66IK1; differential.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0048468; P:cell development; IBA:GO_Central.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; ISS:UniProtKB.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0001840; P:neural plate development; ISS:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0009954; P:proximal/distal pattern formation; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR003893; Iroquois_homeo.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00548; IRO; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..456
FT /note="Iroquois-class homeodomain protein irx-2"
FT /id="PRO_0000388717"
FT DNA_BIND 110..172
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 172..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 434..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..214
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..268
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 456 AA; 49593 MW; D9FCBFEA8CCD86D5 CRC64;
MSYPQGYLYQ PPGSLALYSC PAYGASALAA PRSEELARSS SGSAFSPYPG SAAFTAQAAT
GFSSPLQYSS DPAGFPSYMG SPYDAHTTGM TGALSYHPYG SAAYPYQLND PAYRKNATRD
ATATLKAWLQ EHRKNPYPTK GEKIMLAIIT KMTLTQVSTW FANARRRLKK ENKMTWAPRN
KSEDEDDDEG DGERVKEEQS EKAQDCNETS AEDEGISLHV DSLTDHSCSA DSDGEKLPCR
ATDHLCESGS ESKEKYDDDE DEEEGDEEDR VLPVKPATSS PLTGVEAPIL NHQQDGSPRN
SNKTSLDNGM SPSSQTPASK PKLWSLAEIA TSDHKHSNLG SVLSSATSSA AHNPSYPSSS
LLGRHIYYTS PFYSNYTNYG NFNALQSQGI LRYSSAAVTA NEGLNQTVLS TSSMHKHTSD
SVRTASNQLD QHYRPTNFES KKDPSEVCTV GVQPYP
