IRX3_XENTR
ID IRX3_XENTR Reviewed; 448 AA.
AC Q6NVN3;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Iroquois-class homeodomain protein irx-3;
DE AltName: Full=Iroquois homeobox protein 3 {ECO:0000250|UniProtKB:P78415, ECO:0000312|EMBL:CAJ82766.1};
GN Name=irx3 {ECO:0000312|EMBL:AAH67972.1}; ORFNames=TNeu131p22.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000312|EMBL:CAJ82766.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:CAJ82766.1};
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:CAJ82766.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tail bud {ECO:0000312|EMBL:AAH67972.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19268445; DOI=10.1016/j.ydbio.2009.02.028;
RA Rodriguez-Seguel E., Alarcon P., Gomez-Skarmeta J.L.;
RT "The Xenopus Irx genes are essential for neural patterning and define the
RT border between prethalamus and thalamus through mutual antagonism with the
RT anterior repressors Fezf and Arx.";
RL Dev. Biol. 329:258-268(2009).
CC -!- FUNCTION: Acts partially redundantly with other irx members in neural
CC patterning. Required for formation of the posterior forebrain,
CC midbrain, hindbrain, and to a lesser extent, spinal cord. Both up-
CC regulates and down-regulates gene expression during neural development.
CC Acts early in neural plate development to induce proneural gene
CC expression and specify a neural precursor state. Also up-regulates
CC repressors that prevent neuronal differentiation. Required during at
CC least two stages of pronephros kidney development; during neurula
CC stages, maintains transcription of key renal genes to define the size
CC and identity of the pronephric anlage, probably in part through
CC regulation of bmp-signaling. Subsequently required for proper formation
CC of the intermediate tubule segment of the pronephros.
CC {ECO:0000269|PubMed:19268445}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the neural plate in overlapping
CC patterns with other irx members, which all share an anterior border of
CC expression. Outside the nervous system and at tailbud stages, expressed
CC in the developing otic vesicle, branchial arches, prospective heart
CC region and pronephros. {ECO:0000269|PubMed:19268445}.
CC -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR760274; CAJ82766.1; -; mRNA.
DR EMBL; BC067972; AAH67972.1; -; mRNA.
DR RefSeq; NP_001001216.1; NM_001001216.1.
DR AlphaFoldDB; Q6NVN3; -.
DR SMR; Q6NVN3; -.
DR PaxDb; Q6NVN3; -.
DR DNASU; 407888; -.
DR GeneID; 407888; -.
DR KEGG; xtr:407888; -.
DR CTD; 79191; -.
DR Xenbase; XB-GENE-480486; irx3.
DR eggNOG; KOG0773; Eukaryota.
DR HOGENOM; CLU_042927_0_1_1; -.
DR InParanoid; Q6NVN3; -.
DR OMA; PNENNAP; -.
DR OrthoDB; 814237at2759; -.
DR PhylomeDB; Q6NVN3; -.
DR TreeFam; TF319371; -.
DR Proteomes; UP000008143; Chromosome 4.
DR Proteomes; UP000790000; Unplaced.
DR ExpressionAtlas; Q6NVN3; baseline.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0048468; P:cell development; IBA:GO_Central.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; ISS:UniProtKB.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0001840; P:neural plate development; ISS:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0048793; P:pronephros development; ISS:UniProtKB.
DR GO; GO:0009954; P:proximal/distal pattern formation; IEA:UniProt.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR003893; Iroquois_homeo.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00548; IRO; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..448
FT /note="Iroquois-class homeodomain protein irx-3"
FT /id="PRO_0000386622"
FT DNA_BIND 108..170
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 171..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 387..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..193
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..218
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..250
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 448 AA; 49174 MW; B70831FFB2FE68A0 CRC64;
MSFPQLGYQY IRPLYPSDRQ NVGGTRSGTE LSPAGTLSNV LSSVYGTPYA AAAAAQAYGA
FLPYSAELPI FPQLGSQYEM KDSPGVQHAA FSHPHAAFYP YGQYQFGDPS RPKNATREST
STLKAWLNEH RKNPYPTKGE KIMLAIITKM TLTQVSTWFA NARRRLKKEN KMTWAPRSRT
DEEGNAYGSD HEEDKHEDDE EIDLENIDTE DIESKEDLDD PDTDIHSDSK TDARSDSEAS
DGFEDLNAPE DRLLKSVVGQ RQVLNEEPQD KCALSSDAKA SQPACEQIKL DRIPSSPPLE
NNIPAAHKPK IWSLAETATT PDNPRRSPNT GGSVNTQNLI AQHRLIASPG SRFQGWTGRA
FSAQQLSLLN SAHFLQGLSV SHTALGSGTA SFPKAAEPKH STDSLTDRSS TVDIEKKIPV
LNTAFQPVQR RSQNQLDAAM ILSALSSS
