ID   IRX4_CHICK              Reviewed;         485 AA.
AC   Q9YGS0;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Iroquois-class homeodomain protein IRX-4;
DE   AltName: Full=Iroquois homeobox protein 4;
GN   Name=IRX4;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryonic retina;
RX   PubMed=10024241; DOI=10.1126/science.283.5405.1161;
RA   Bao Z.-Z., Bruneau B.G., Seidman J.G., Seidman C.E., Cepko C.L.;
RT   "Regulation of chamber-specific gene expression in the developing heart by
RT   Irx4.";
RL   Science 283:1161-1164(1999).
CC   -!- FUNCTION: Regulates the chamber-specific expression of myosin isoforms
CC       by activating the expression of the ventricle myosin heavy chain-1
CC       (Vmhc1) and suppressing the expression of the atrial myosin heavy
CC       chain-1 (Amhc1) in the ventricles. May play a critical role in
CC       establishing chamber-specific gene expression in the developing heart.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC   -!- TISSUE SPECIFICITY: Ventricles of the heart, developing feather buds,
CC       retina, hindbrain.
CC   -!- DEVELOPMENTAL STAGE: All stages of heart development.
CC   -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000305}.
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DR   EMBL; AF091504; AAD16100.1; -; mRNA.
DR   RefSeq; NP_001001744.1; NM_001001744.1.
DR   RefSeq; XP_015137809.1; XM_015282323.1.
DR   RefSeq; XP_015137810.1; XM_015282324.1.
DR   AlphaFoldDB; Q9YGS0; -.
DR   SMR; Q9YGS0; -.
DR   STRING; 9031.ENSGALP00000036193; -.
DR   Ensembl; ENSGALT00000036981; ENSGALP00000036193; ENSGALG00000022896.
DR   GeneID; 373974; -.
DR   KEGG; gga:373974; -.
DR   CTD; 50805; -.
DR   VEuPathDB; HostDB:geneid_373974; -.
DR   GeneTree; ENSGT00940000158596; -.
DR   InParanoid; Q9YGS0; -.
DR   OMA; QQAKSCY; -.
DR   OrthoDB; 814237at2759; -.
DR   PhylomeDB; Q9YGS0; -.
DR   PRO; PR:Q9YGS0; -.
DR   Proteomes; UP000000539; Chromosome 2.
DR   Bgee; ENSGALG00000022896; Expressed in heart.
DR   ExpressionAtlas; Q9YGS0; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0048468; P:cell development; IBA:GO_Central.
DR   GO; GO:0007507; P:heart development; IEA:Ensembl.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR008422; Homeobox_KN_domain.
DR   InterPro; IPR003893; Iroquois_homeo.
DR   Pfam; PF05920; Homeobox_KN; 1.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00548; IRO; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..485
FT                   /note="Iroquois-class homeodomain protein IRX-4"
FT                   /id="PRO_0000049159"
FT   DNA_BIND        142..203
FT                   /note="Homeobox; TALE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          206..313
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        233..253
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        279..296
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   485 AA;  52774 MW;  81F5A72F9B30C208 CRC64;
     MSYPQFGYPY SSAPQFLMST NSLTPCCESG GRTLPESGPA APAQTPVYCP VYESRLLATA
     RHELNSAAAL GVYGGPYGGP QGYGNYVTYG TEAPAFYSLN TLEAKDGGGS AHAGISPAAA
     YYPYEHSLSQ YQYDRYGAMD GGTRRKNATR ETTSTLKAWL QEHRKNPYPT KGEKIMLAII
     TKMTLTQVST WFANARRRLK KENKMTWPPR NKCSDEKRPY EEEEEEEEEC SQEDAMKSEK
     AEEPTGKEEK ELELSDLEDL DAAESESSEC EMRRPFPHPH PHPLPGGGPP PRAAEPPAAA
     AEEEEEEAAE RARGCLKPAA EECEAALLGA RPRGCEAKLC FPQGQPLLEA KPRIWSLAHT
     ATSLNQAEYP SCMLKRSGGS AAAAVSAPVS VMDRHQDSPV TNLRNWVDGV FHDPLFRHST
     LNQALSNTTV SWATTKGAIL ETGALGRSVG NGANVLKGQL ANLAHQDSSK EFLAFPKAGS
     KMFCS