IRX4_MOUSE
ID IRX4_MOUSE Reviewed; 515 AA.
AC Q9QY61; A0JNT5; Q3V1B6;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Iroquois-class homeodomain protein IRX-4;
DE AltName: Full=Homeodomain protein IRXA3;
DE AltName: Full=Iroquois homeobox protein 4;
GN Name=Irx4; Synonyms=Irxa3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROBABLE FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryonic heart;
RX PubMed=10625552; DOI=10.1006/dbio.1999.9548;
RA Bruneau B.G., Bao Z.-Z., Tanaka M., Schott J.-J., Izumo S., Cepko C.L.,
RA Seidman J.G., Seidman C.E.;
RT "Cardiac expression of the ventricle-specific homeobox gene Irx4 is
RT modulated by Nkx2-5 and dHand.";
RL Dev. Biol. 217:266-277(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=FVB/N; TISSUE=Embryonic heart;
RX PubMed=10926765; DOI=10.1006/dbio.2000.9801;
RA Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W.,
RA Moorman A.F.M.;
RT "Patterning the embryonic heart: identification of five mouse Iroquois
RT homeobox genes in the developing heart.";
RL Dev. Biol. 224:263-274(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP LACK OF RENAL EXPRESSION.
RX PubMed=17875669; DOI=10.1101/gad.450707;
RA Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.;
RT "The prepattern transcription factor Irx3 directs nephron segment
RT identity.";
RL Genes Dev. 21:2358-2370(2007).
CC -!- FUNCTION: Likely to be an important mediator of ventricular
CC differentiation during cardiac development.
CC -!- SUBUNIT: Interacts with the vitamin D receptor VDR but doesn't affect
CC its transactivation activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing central nervous system,
CC skin, and vibrissae, but predominantly expressed in the cardiac
CC ventricles of the developing heart. Not expressed in the developing
CC metanephric kidney or adult kidney. {ECO:0000269|PubMed:10625552,
CC ECO:0000269|PubMed:10926765}.
CC -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000305}.
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DR EMBL; AF124732; AAF23886.1; -; mRNA.
DR EMBL; AK132571; BAE21235.1; -; mRNA.
DR EMBL; CH466563; EDL37045.1; -; Genomic_DNA.
DR EMBL; BC126942; AAI26943.1; -; mRNA.
DR CCDS; CCDS26629.1; -.
DR RefSeq; NP_061373.1; NM_018885.2.
DR RefSeq; XP_006517355.1; XM_006517292.2.
DR RefSeq; XP_006517356.1; XM_006517293.2.
DR RefSeq; XP_006517357.1; XM_006517294.3.
DR RefSeq; XP_006517358.1; XM_006517295.1.
DR RefSeq; XP_006517359.1; XM_006517296.1.
DR AlphaFoldDB; Q9QY61; -.
DR SMR; Q9QY61; -.
DR STRING; 10090.ENSMUSP00000022095; -.
DR iPTMnet; Q9QY61; -.
DR PhosphoSitePlus; Q9QY61; -.
DR PaxDb; Q9QY61; -.
DR PRIDE; Q9QY61; -.
DR Antibodypedia; 9116; 74 antibodies from 17 providers.
DR DNASU; 50916; -.
DR Ensembl; ENSMUST00000022095; ENSMUSP00000022095; ENSMUSG00000021604.
DR Ensembl; ENSMUST00000176684; ENSMUSP00000134738; ENSMUSG00000021604.
DR GeneID; 50916; -.
DR KEGG; mmu:50916; -.
DR UCSC; uc007rdf.2; mouse.
DR CTD; 50805; -.
DR MGI; MGI:1355275; Irx4.
DR VEuPathDB; HostDB:ENSMUSG00000021604; -.
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000158596; -.
DR HOGENOM; CLU_042927_1_1_1; -.
DR InParanoid; Q9QY61; -.
DR OMA; QQAKSCY; -.
DR OrthoDB; 814237at2759; -.
DR PhylomeDB; Q9QY61; -.
DR TreeFam; TF319371; -.
DR BioGRID-ORCS; 50916; 2 hits in 74 CRISPR screens.
DR PRO; PR:Q9QY61; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9QY61; protein.
DR Bgee; ENSMUSG00000021604; Expressed in lip and 66 other tissues.
DR Genevisible; Q9QY61; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; ISS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048468; P:cell development; IBA:GO_Central.
DR GO; GO:0048561; P:establishment of animal organ orientation; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR003893; Iroquois_homeo.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00548; IRO; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox; Nucleus;
KW Reference proteome.
FT CHAIN 1..515
FT /note="Iroquois-class homeodomain protein IRX-4"
FT /id="PRO_0000049158"
FT DNA_BIND 144..205
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 205..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..307
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..226
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 398..413
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 215
FT /note="C -> Y (in Ref. 3; BAE21235)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 515 AA; 54701 MW; A3E7C67B7D5334BE CRC64;
MSYPQFGYPY SSAPQFLMTT NSLSTCCESG GRTLADSGPA ASAQAPVYCP VYESRLLATA
RHELNSAAAL GVYGSPYGSS QGYGNYVTYG SEASAFYSLN SFESKDGTGS SHAGLPPTAA
AAYYPYEPAL SQYPYDRYGT VDSGTRRKNA TRETTSTLKA WLQEHRKNPY PTKGEKIMLA
IITKMTLTQV STWFANARRR LKKENKMTWP PRNKCADEKR PYGEGEEEEA GEEESREEPL
KSAKSEGHAG KDDKELELSD LEDFDPLDAE TSECELKTPF QSLDSGPERI PASSDGPGTG
KEASTTLRMP LGTAGGAVMD GDLERARNCL RSTVVVPDSG AEGGPPACEA KLTFAQAGAP
PNLETKPRIW SLAHTATAAA ATALSQTEFP SCMLKRQGPT GVSATTPASS PAVTAPSGAL
DRHQDSPVTS LRNWVDGVFH DPILRHSTLN QAWATAKGAL LDPGPLGRNL GAGTNVLTTP
LACSFPPTVP QDVPPAGASR ELLATPKAGG KPFCT
