ID   IRX4_XENTR              Reviewed;         496 AA.
AC   Q688D0;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Iroquois-class homeodomain protein irx-4 {ECO:0000250|UniProtKB:Q90XW6};
DE   AltName: Full=Iroquois homeobox protein 4 {ECO:0000250|UniProtKB:Q90XW6};
GN   Name=irx4 {ECO:0000250|UniProtKB:Q90XW6};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1] {ECO:0000312|EMBL:AAU12854.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Qin S., Dors M., Johnson E., Bloom S., Hood L., Rowen L.;
RT   "Sequence of Xenopus tropicalis development genes.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=19268445; DOI=10.1016/j.ydbio.2009.02.028;
RA   Rodriguez-Seguel E., Alarcon P., Gomez-Skarmeta J.L.;
RT   "The Xenopus Irx genes are essential for neural patterning and define the
RT   border between prethalamus and thalamus through mutual antagonism with the
RT   anterior repressors Fezf and Arx.";
RL   Dev. Biol. 329:258-268(2009).
CC   -!- FUNCTION: Acts partially redundantly with other irx members in neural
CC       patterning. Required for formation of the posterior forebrain,
CC       midbrain, hindbrain, and to a lesser extent, spinal cord. Patterns the
CC       neuroectoderm in both the anterior/posterior and dorsal/ventral axes.
CC       Does not appear to play a role in pronephros kidney development.
CC       {ECO:0000250|UniProtKB:Q90XW6, ECO:0000269|PubMed:19268445}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in the neural plate in overlapping
CC       patterns with other irx members, which all share an anterior border of
CC       expression. Broadly expressed in the tailbud rhombencephalon
CC       (hindbrain). Outside the nervous system and at tailbud stages,
CC       expressed in the developing otic vesicle, branchial arches and
CC       prospective heart region. {ECO:0000269|PubMed:19268445}.
CC   -!- SIMILARITY: Belongs to the TALE/IRO homeobox family. {ECO:0000255}.
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DR   EMBL; AC149071; AAU12854.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q688D0; -.
DR   SMR; Q688D0; -.
DR   PaxDb; Q688D0; -.
DR   Ensembl; ENSXETT00000019512; ENSXETP00000019512; ENSXETG00000008892.
DR   eggNOG; KOG0773; Eukaryota.
DR   HOGENOM; CLU_042927_1_1_1; -.
DR   InParanoid; Q688D0; -.
DR   OMA; DRTKICH; -.
DR   TreeFam; TF319371; -.
DR   Proteomes; UP000008143; Genome assembly.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000008892; Expressed in heart and 2 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0007420; P:brain development; IEA:UniProt.
DR   GO; GO:0048468; P:cell development; IBA:GO_Central.
DR   GO; GO:0009953; P:dorsal/ventral pattern formation; IEA:UniProt.
DR   GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0009954; P:proximal/distal pattern formation; IEA:UniProt.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR008422; Homeobox_KN_domain.
DR   InterPro; IPR003893; Iroquois_homeo.
DR   Pfam; PF05920; Homeobox_KN; 1.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00548; IRO; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Homeobox;
KW   Neurogenesis; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..496
FT                   /note="Iroquois-class homeodomain protein irx-4"
FT                   /id="PRO_0000388720"
FT   DNA_BIND        141..203
FT                   /note="Homeobox; TALE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          203..236
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        203..219
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   496 AA;  55393 MW;  40F1E43C7C5B7A9A CRC64;
     MSYPQFGYPY SSTPQFLMTT NSLSTCCESS GRSLSDSAAA ASAQTPVYCP VYESRLLATA
     RHELNSAAAL GVYGSPYTST QGYGNYVTYG ADASAFYSLN AFESKDGTGS AHAGIPQTAA
     YYPYEHTLSQ YQYDRYGTMD GSTRRKNATR ETTSTLKAWL QEHRKNPYPT KGEKIMLAII
     TKMTLTQVST WFANARRRLK KENKMTWPPR NKCSDEKRPY DEEEEEEEEE DSQKATIKNE
     KKIVDEEVAR EDKALDLSDL EDFDAIESES SECELKQPFH HQPQDGHQLR QRDCVNDHCK
     DVILKMPITP AANQELDRTK ICHKPGVDQC EQEVLRGRQR GGESKACFQQ QQILDSKPRI
     WSLAHTATSL NQTEYPSCML KHQGLSSPSS SSSSSAVSTP VCVIDRRQDS PVTSLRNWVD
     GVFHDPLFRH STLNQALTNT TVSWATTKGT LIDSGSLGRS VGNPTNAIKG QLPNIPHDTN
     KEFIAFQKSG SKMFCS