IRX9H_ARATH
ID IRX9H_ARATH Reviewed; 394 AA.
AC Q9SXC4; Q8LD18; Q9SFZ7;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Probable beta-1,4-xylosyltransferase IRX9H;
DE EC=2.4.2.-;
DE AltName: Full=Protein IRREGULAR XYLEM 9 homolog;
DE AltName: Full=Xylan xylosyltransferase IRX9H;
GN Name=IRX9H; OrderedLocusNames=At1g27600; ORFNames=T17H3.10, T22C5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-394.
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20335400; DOI=10.1104/pp.110.155309;
RA Lee C., Teng Q., Huang W., Zhong R., Ye Z.H.;
RT "The Arabidopsis family GT43 glycosyltransferases form two functionally
RT nonredundant groups essential for the elongation of glucuronoxylan
RT backbone.";
RL Plant Physiol. 153:526-541(2010).
RN [5]
RP FUNCTION.
RX PubMed=20424005; DOI=10.1104/pp.110.154971;
RA Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P.,
RA Marchant A.;
RT "Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of
RT glycosyltransferase genes reveals critical contributions to biosynthesis of
RT the hemicellulose glucuronoxylan.";
RL Plant Physiol. 153:542-554(2010).
CC -!- FUNCTION: Involved in the synthesis of the hemicellulose
CC glucuronoxylan, a major component of secondary cell walls. Probably
CC involved in the elongation of glucuronoxylan xylosyl backbone.
CC {ECO:0000269|PubMed:20335400, ECO:0000269|PubMed:20424005}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000305|PubMed:20335400}; Single-pass type II membrane protein
CC {ECO:0000305|PubMed:20335400}.
CC -!- TISSUE SPECIFICITY: Expressed in developing interfascicular fibers and
CC xylem cells in stems and developing secondary xylem in roots.
CC {ECO:0000269|PubMed:20335400}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 43 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD45998.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF24965.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM64331.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC005916; AAD45998.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC012375; AAF24965.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30852.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30853.1; -; Genomic_DNA.
DR EMBL; AY086258; AAM64331.1; ALT_INIT; mRNA.
DR RefSeq; NP_564290.2; NM_102525.3.
DR RefSeq; NP_973922.1; NM_202193.1.
DR AlphaFoldDB; Q9SXC4; -.
DR SMR; Q9SXC4; -.
DR BioGRID; 24887; 1.
DR STRING; 3702.AT1G27600.1; -.
DR CAZy; GT43; Glycosyltransferase Family 43.
DR iPTMnet; Q9SXC4; -.
DR PaxDb; Q9SXC4; -.
DR PRIDE; Q9SXC4; -.
DR ProteomicsDB; 247054; -.
DR EnsemblPlants; AT1G27600.1; AT1G27600.1; AT1G27600.
DR EnsemblPlants; AT1G27600.2; AT1G27600.2; AT1G27600.
DR GeneID; 839652; -.
DR Gramene; AT1G27600.1; AT1G27600.1; AT1G27600.
DR Gramene; AT1G27600.2; AT1G27600.2; AT1G27600.
DR KEGG; ath:AT1G27600; -.
DR Araport; AT1G27600; -.
DR TAIR; locus:2196899; AT1G27600.
DR eggNOG; KOG1476; Eukaryota.
DR HOGENOM; CLU_044006_1_1_1; -.
DR InParanoid; Q9SXC4; -.
DR OMA; AFQAYFL; -.
DR OrthoDB; 901158at2759; -.
DR BioCyc; ARA:AT1G27600-MON; -.
DR PRO; PR:Q9SXC4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SXC4; baseline and differential.
DR Genevisible; Q9SXC4; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015018; F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; IEA:InterPro.
DR GO; GO:0042285; F:xylosyltransferase activity; IMP:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010417; P:glucuronoxylan biosynthetic process; IMP:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR GO; GO:0045492; P:xylan biosynthetic process; IMP:TAIR.
DR CDD; cd00218; GlcAT-I; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005027; Glyco_trans_43.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR10896; PTHR10896; 1.
DR Pfam; PF03360; Glyco_transf_43; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..394
FT /note="Probable beta-1,4-xylosyltransferase IRX9H"
FT /id="PRO_0000407564"
FT TOPO_DOM 1..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..394
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 280
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 309
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 394 AA; 45187 MW; B6D2C629147BA6E5 CRC64;
MASIRRTLSP MYHDRSHENG GSHKGFTIGG SSSKHNSSQF LSYLTKLLGV TSDPKSSRRG
PWRRPFYQFL VFFLLGFVLG LTPFGKMEDV NGSDRFSFEI KQPYVEERLE NRKREEAAVD
AVSFVAETEN GKKEVNFVPK KLLIVVTPTY NRAMQAYYLN RVAQTLRLVE SPVLWIVVEG
NVASFETSEI LRKTGVMYRH LVCKRNMTSI KDRGVHQRNT ALEHIELHKL DGIVYFADDD
NIYSLELFQS LRQISRFGTW PVAMLAQSKN KAILEGPVCN GSQVIGWHTN EKSKRLRRFH
VDMSGFAFNS TILWDPKRWR RPFSHPTRQL DTVKEGFQET SFIEQVVADE SEMEGVPPAC
SSILNWHLHL DALDVPYPQG WAIQKNLQAL ITMK
