ID   ISA2_SCHPO              Reviewed;         205 AA.
AC   O43045;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Iron-sulfur assembly protein 2;
GN   Name=isa2; ORFNames=SPBC3B9.17;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Involved in the assembly of mitochondrial and cytoplasmic
CC       iron-sulfur proteins. Probably involved in the binding of an
CC       intermediate of Fe/S cluster assembly (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HesB/IscA family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA17797.1; -; Genomic_DNA.
DR   PIR; T40356; T40356.
DR   RefSeq; NP_596675.1; NM_001022597.2.
DR   AlphaFoldDB; O43045; -.
DR   SMR; O43045; -.
DR   BioGRID; 277500; 1.
DR   STRING; 4896.SPBC3B9.17.1; -.
DR   MaxQB; O43045; -.
DR   PaxDb; O43045; -.
DR   EnsemblFungi; SPBC3B9.17.1; SPBC3B9.17.1:pep; SPBC3B9.17.
DR   GeneID; 2540984; -.
DR   KEGG; spo:SPBC3B9.17; -.
DR   PomBase; SPBC3B9.17; isa2.
DR   VEuPathDB; FungiDB:SPBC3B9.17; -.
DR   eggNOG; KOG1119; Eukaryota.
DR   HOGENOM; CLU_069054_1_0_1; -.
DR   InParanoid; O43045; -.
DR   PhylomeDB; O43045; -.
DR   Reactome; R-SPO-1362409; Mitochondrial iron-sulfur cluster biogenesis.
DR   PRO; PR:O43045; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IDA:PomBase.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; ISO:PomBase.
DR   GO; GO:0044572; P:[4Fe-4S] cluster assembly; ISS:PomBase.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR   GO; GO:0106035; P:protein maturation by [4Fe-4S] cluster transfer; IBA:GO_Central.
DR   Gene3D; 2.60.300.12; -; 1.
DR   InterPro; IPR000361; FeS_biogenesis.
DR   InterPro; IPR016092; FeS_cluster_insertion.
DR   InterPro; IPR017870; FeS_cluster_insertion_CS.
DR   InterPro; IPR035903; HesB-like_dom_sf.
DR   Pfam; PF01521; Fe-S_biosyn; 1.
DR   SUPFAM; SSF89360; SSF89360; 1.
DR   TIGRFAMs; TIGR00049; TIGR00049; 1.
DR   PROSITE; PS01152; HESB; 1.
PE   3: Inferred from homology;
KW   Iron; Metal-binding; Mitochondrion; Reference proteome.
FT   CHAIN           1..205
FT                   /note="Iron-sulfur assembly protein 2"
FT                   /id="PRO_0000077037"
FT   BINDING         131
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         196
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         198
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
SQ   SEQUENCE   205 AA;  23239 MW;  03A02F4B2A2F2F59 CRC64;
     MLMKSRVNLL FKGPFFFRER CFQTLLNPVI AIRQLSHGSL RNSRSCRIDA KSNTLTKEIF
     PKINNEFKIK NRFYSSKLAN DQFTLQEQCD EKGIPFILYV KDSAKKQLEK IAERKPEENS
     VLRVTVDGGG CHGYQVSFRM DNKIGNADTV FVRGKARVVA DNISLPLISG SEIEYTNELI
     GSSFQLLNNP RAKTSCGCNV SFDVE