ID   ISAA_STAAC              Reviewed;         233 AA.
AC   Q5HCY1;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Probable transglycosylase IsaA;
DE            EC=3.2.-.-;
DE   AltName: Full=Immunodominant staphylococcal antigen A;
DE   Flags: Precursor;
GN   Name=isaA; OrderedLocusNames=SACOL2584;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 30-36, AND SUBCELLULAR LOCATION.
RX   PubMed=11681202;
RX   DOI=10.1002/1615-9861(200104)1:4<480::aid-prot480>3.0.co;2-o;
RA   Ziebandt A.-K., Weber H., Rudolph J., Schmid R., Hoeper D., Engelmann S.,
RA   Hecker M.;
RT   "Extracellular proteins of Staphylococcus aureus and the role of SarA and
RT   sigma B.";
RL   Proteomics 1:480-493(2001).
CC   -!- FUNCTION: Is able to cleave peptidoglycan. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11681202}.
CC   -!- SIMILARITY: Belongs to the transglycosylase family. IsaA subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000046; AAW38586.1; -; Genomic_DNA.
DR   RefSeq; WP_000751267.1; NC_002951.2.
DR   AlphaFoldDB; Q5HCY1; -.
DR   SMR; Q5HCY1; -.
DR   EnsemblBacteria; AAW38586; AAW38586; SACOL2584.
DR   KEGG; sac:SACOL2584; -.
DR   HOGENOM; CLU_099865_0_0_9; -.
DR   OMA; MWNTIVM; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR008258; Transglycosylase_SLT_dom_1.
DR   Pfam; PF01464; SLT; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosidase; Hydrolase; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000269|PubMed:11681202"
FT   CHAIN           30..233
FT                   /note="Probable transglycosylase IsaA"
FT                   /id="PRO_0000045204"
SQ   SEQUENCE   233 AA;  24203 MW;  A76C4C225D17DEB2 CRC64;
     MKKTIMASSL AVALGVTGYA AGTGHQAHAA EVNVDQAHLV DLAHNHQDQL NAAPIKDGAY
     DIHFVKDGFQ YNFTSNGTTW SWSYEAANGQ TAGFSNVAGA DYTTSYNQGS NVQSVSYNAQ
     SSNSNVEAVS APTYHNYSTS TTSSSVRLSN GNTAGATGSS AAQIMAQRTG VSASTWAAII
     ARESNGQVNA YNPSGASGLF QTMPGWGPTN TVDQQINAAV KAYKAQGLGA WGF