ID   ISAA_STAAU              Reviewed;         233 AA.
AC   P60158; Q9LAB6; Q9LBE4;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2003, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Probable transglycosylase IsaA;
DE            EC=3.2.-.-;
DE   AltName: Full=Immunodominant staphylococcal antigen A;
DE   AltName: Full=SAI-2;
DE   AltName: Full=Secretory protein SAI-B;
DE   Flags: Precursor;
GN   Name=isaA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-59, SUBCELLULAR
RP   LOCATION, AND INDUCTION.
RC   STRAIN=M804;
RX   PubMed=11024354; DOI=10.1111/j.1574-695x.2000.tb01517.x;
RA   Lorenz U., Ohlsen K., Karch H., Hecker M., Thiede A., Hacker J.;
RT   "Human antibody response during sepsis against targets expressed by
RT   methicillin resistant Staphylococcus aureus.";
RL   FEMS Immunol. Med. Microbiol. 29:145-153(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530;
RA   Sakata N., Wadstrom T., Yamazaki K., Mukai T.;
RT   "Staphylococcal secretory protein.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INDUCTION, AND EXPRESSION.
RC   STRAIN=ATCC 6538P / DSM 346 / JCM 2151 / NBRC 12732 / NCIMB 8625 / NCTC
RC   7447 / NRRL B-313 / FDA 209P;
RX   PubMed=17328763; DOI=10.1111/j.1574-695x.2006.00200.x;
RA   Sakata N., Mukai T.;
RT   "Production profile of the soluble lytic transglycosylase homologue in
RT   Staphylococcus aureus during bacterial proliferation.";
RL   FEMS Immunol. Med. Microbiol. 49:288-295(2007).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=98S-1241;
RX   PubMed=17997515; DOI=10.1021/pr070521m;
RA   Drummelsmith J., Winstall E., Bergeron M.G., Poirier G.G., Ouellette M.;
RT   "Comparative proteomics analyses reveal a potential biomarker for the
RT   detection of vancomycin-intermediate Staphylococcus aureus strains.";
RL   J. Proteome Res. 6:4690-4702(2007).
CC   -!- FUNCTION: Is able to cleave peptidoglycan. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11024354}.
CC   -!- INDUCTION: Protein expression was detectable in the early exponential
CC       growth phase and its concentration constantly increased until the early
CC       stationary phase. Gene expression also greatly and rapidly increased
CC       during the early exponential growth, about 400-fold increase compared
CC       with its level in the cells before inoculation. Subsequently, the
CC       number of transcripts was reduced and a constant level was maintained
CC       until the last stage of exponential growth phase. In the stationary
CC       phase, the number of transcripts gradually decreased.
CC       {ECO:0000269|PubMed:11024354, ECO:0000269|PubMed:17328763}.
CC   -!- SIMILARITY: Belongs to the transglycosylase family. IsaA subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF144681; AAF66691.1; -; Genomic_DNA.
DR   EMBL; AB042839; BAA95959.1; -; Genomic_DNA.
DR   RefSeq; WP_000751267.1; NZ_WSFM01000002.1.
DR   AlphaFoldDB; P60158; -.
DR   SMR; P60158; -.
DR   OMA; MWNTIVM; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR008258; Transglycosylase_SLT_dom_1.
DR   Pfam; PF01464; SLT; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosidase; Hydrolase; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000269|PubMed:11024354"
FT   CHAIN           30..233
FT                   /note="Probable transglycosylase IsaA"
FT                   /id="PRO_0000021530"
FT   CONFLICT        172
FT                   /note="S -> P (in Ref. 2; BAA95959)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   233 AA;  24203 MW;  A76C4C225D17DEB2 CRC64;
     MKKTIMASSL AVALGVTGYA AGTGHQAHAA EVNVDQAHLV DLAHNHQDQL NAAPIKDGAY
     DIHFVKDGFQ YNFTSNGTTW SWSYEAANGQ TAGFSNVAGA DYTTSYNQGS NVQSVSYNAQ
     SSNSNVEAVS APTYHNYSTS TTSSSVRLSN GNTAGATGSS AAQIMAQRTG VSASTWAAII
     ARESNGQVNA YNPSGASGLF QTMPGWGPTN TVDQQINAAV KAYKAQGLGA WGF