APRR9_ARATH
ID APRR9_ARATH Reviewed; 468 AA.
AC Q8L500; O81035; Q9LKL1;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Two-component response regulator-like APRR9;
DE AltName: Full=Pseudo-response regulator 9;
GN Name=APRR9; OrderedLocusNames=At2g46790; ORFNames=F19D11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=11100772; DOI=10.1093/pcp/pcd043;
RA Matsushika A., Makino S., Kojima M., Mizuno T.;
RT "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response
RT regulators in Arabidopsis thaliana: insight into the plant circadian
RT clock.";
RL Plant Cell Physiol. 41:1002-1012(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10926537; DOI=10.1126/science.289.5480.768;
RA Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P.,
RA Panda S., Kreps J.A., Kay S.A.;
RT "Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response
RT regulator homolog.";
RL Science 289:768-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=10945350; DOI=10.1093/pcp/41.6.791;
RA Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T.,
RA Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.;
RT "Genes encoding pseudo-response regulators: insight into His-to-Asp
RT phosphorelay and circadian rhythm in Arabidopsis thaliana.";
RL Plant Cell Physiol. 41:791-803(2000).
RN [7]
RP PHOSPHORYLATION.
RX PubMed=18562312; DOI=10.1074/jbc.m803471200;
RA Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R.,
RA Somers D.E.;
RT "Post-translational regulation of the Arabidopsis circadian clock through
RT selective proteolysis and phosphorylation of pseudo-response regulator
RT proteins.";
RL J. Biol. Chem. 283:23073-23083(2008).
RN [8]
RP REGULATION.
RX PubMed=20962777; DOI=10.1038/nature09470;
RA Sanchez S.E., Petrillo E., Beckwith E.J., Zhang X., Rugnone M.L.,
RA Hernando C.E., Cuevas J.C., Godoy Herz M.A., Depetris-Chauvin A.,
RA Simpson C.G., Brown J.W., Cerdan P.D., Borevitz J.O., Mas P., Ceriani M.F.,
RA Kornblihtt A.R., Yanovsky M.J.;
RT "A methyl transferase links the circadian clock to the regulation of
RT alternative splicing.";
RL Nature 468:112-116(2010).
RN [9]
RP FUNCTION.
RX PubMed=20233950; DOI=10.1105/tpc.109.072892;
RA Nakamichi N., Kiba T., Henriques R., Mizuno T., Chua N.H., Sakakibara H.;
RT "PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in
RT the Arabidopsis circadian clock.";
RL Plant Cell 22:594-605(2010).
RN [10]
RP FUNCTION.
RX PubMed=21098730; DOI=10.1105/tpc.110.079087;
RA Salome P.A., Weigel D., McClung C.R.;
RT "The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and
RT PRR9 in temperature compensation.";
RL Plant Cell 22:3650-3661(2010).
RN [11]
RP FUNCTION, AND INDUCTION.
RX PubMed=21357491; DOI=10.1105/tpc.110.081661;
RA Wang Y., Wu J.F., Nakamichi N., Sakakibara H., Nam H.G., Wu S.H.;
RT "LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive
RT feedback regulatory loop in the Arabidopsis circadian clock.";
RL Plant Cell 23:486-498(2011).
RN [12]
RP REGULATION.
RX PubMed=22307044; DOI=10.4161/psb.18766;
RA Chow B.Y., Helfer A., Nusinow D.A., Kay S.A.;
RT "ELF3 recruitment to the PRR9 promoter requires other Evening Complex
RT members in the Arabidopsis circadian clock.";
RL Plant Signal. Behav. 7:170-173(2012).
RN [13]
RP REGULATION.
RX PubMed=22327739; DOI=10.1105/tpc.111.093807;
RA Herrero E., Kolmos E., Bujdoso N., Yuan Y., Wang M., Berns M.C.,
RA Uhlworm H., Coupland G., Saini R., Jaskolski M., Webb A., Goncalves J.,
RA Davis S.J.;
RT "EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains
RT the Arabidopsis circadian clock.";
RL Plant Cell 24:428-443(2012).
RN [14]
RP REGULATION.
RX PubMed=22942380; DOI=10.1105/tpc.112.100081;
RA Wang X., Wu F., Xie Q., Wang H., Wang Y., Yue Y., Gahura O., Ma S., Liu L.,
RA Cao Y., Jiao Y., Puta F., McClung C.R., Xu X., Ma L.;
RT "SKIP is a component of the spliceosome linking alternative splicing and
RT the circadian clock in Arabidopsis.";
RL Plant Cell 24:3278-3295(2012).
RN [15]
RP FUNCTION.
RX PubMed=23027938; DOI=10.1073/pnas.1205156109;
RA Nakamichi N., Kiba T., Kamioka M., Suzuki T., Yamashino T., Higashiyama T.,
RA Sakakibara H., Mizuno T.;
RT "Transcriptional repressor PRR5 directly regulates clock-output pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:17123-17128(2012).
RN [16]
RP INDUCTION BY HEAT-SHOCK.
RX PubMed=23933882; DOI=10.1105/tpc.113.114306;
RA Karayekov E., Sellaro R., Legris M., Yanovsky M.J., Casal J.J.;
RT "Heat shock-induced fluctuations in clock and light signaling enhance
RT phytochrome B-mediated Arabidopsis deetiolation.";
RL Plant Cell 25:2892-2906(2013).
CC -!- FUNCTION: Transcriptional repressor of CCA1 and LHY, and positive
CC regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering
CC response and temperature compensation. Involved in the positive and
CC negative feedback loops of the circadian clock. Expression of several
CC members of the ARR-like family is controlled by circadian rhythm.
CC Regulated at the transcriptional level by a corepressor complex
CC consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately
CC act on the upstream region of the target genes to repress their
CC expression from noon until midnight. The particular coordinated
CC sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to
CC circadian waves that may be at the basis of the endogenous circadian
CC clock. {ECO:0000269|PubMed:11100772, ECO:0000269|PubMed:20233950,
CC ECO:0000269|PubMed:21098730, ECO:0000269|PubMed:21357491,
CC ECO:0000269|PubMed:23027938}.
CC -!- INTERACTION:
CC Q8L500; Q9SJU5: BBX18; NbExp=3; IntAct=EBI-7920168, EBI-15192077;
CC Q8L500; C0SVM5: BBX19; NbExp=3; IntAct=EBI-7920168, EBI-4430993;
CC Q8L500; Q8LCG7: NFYC2; NbExp=3; IntAct=EBI-7920168, EBI-2125983;
CC Q8L500; Q9FMV5: NFYC4; NbExp=3; IntAct=EBI-7920168, EBI-2466018;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8L500-1; Sequence=Displayed;
CC -!- INDUCTION: Up-regulated by heat-shock. {ECO:0000269|PubMed:21357491,
CC ECO:0000269|PubMed:23933882}.
CC -!- PTM: Phosphorylated. Phosphorylation varies throughout the diurnal
CC cycle. {ECO:0000269|PubMed:18562312}.
CC -!- MISCELLANEOUS: Regulated at the level of mRNA maturation and
CC alternative splicing by SKIP (PubMed:22942380) and PRMT5
CC (PubMed:20962777). The expression of APRR9, APRR7, and APRR5 requires
CC the presence of LWD1 and/or LWD2, indicating the existence of a
CC positive feedback loop within the circadian clock (PubMed:21357491).
CC {ECO:0000305|PubMed:20962777, ECO:0000305|PubMed:21357491,
CC ECO:0000305|PubMed:22942380}.
CC -!- SIMILARITY: Belongs to the ARR-like family. {ECO:0000305}.
CC -!- CAUTION: Lacks the phospho-accepting Asp (here Glu-89), present in the
CC receiver domain, which is one of the conserved features of two-
CC component response regulators (ARRs) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM20527.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAM91256.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB046953; BAB13741.1; -; mRNA.
DR EMBL; AF272040; AAF86253.1; -; mRNA.
DR EMBL; AC005310; AAC33497.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10754.1; -; Genomic_DNA.
DR EMBL; AY099676; AAM20527.1; ALT_FRAME; mRNA.
DR EMBL; AY128856; AAM91256.1; ALT_FRAME; mRNA.
DR PIR; T02680; T02680.
DR RefSeq; NP_001325091.1; NM_001337214.1.
DR RefSeq; NP_001325092.1; NM_001337213.1.
DR RefSeq; NP_566085.1; NM_130245.5. [Q8L500-1]
DR AlphaFoldDB; Q8L500; -.
DR SMR; Q8L500; -.
DR BioGRID; 4627; 18.
DR IntAct; Q8L500; 12.
DR MINT; Q8L500; -.
DR STRING; 3702.AT2G46790.1; -.
DR PaxDb; Q8L500; -.
DR PRIDE; Q8L500; -.
DR EnsemblPlants; AT2G46790.1; AT2G46790.1; AT2G46790. [Q8L500-1]
DR GeneID; 819292; -.
DR Gramene; AT2G46790.1; AT2G46790.1; AT2G46790. [Q8L500-1]
DR KEGG; ath:AT2G46790; -.
DR Araport; AT2G46790; -.
DR TAIR; locus:2044376; AT2G46790.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_041215_0_0_1; -.
DR InParanoid; Q8L500; -.
DR OMA; NNKLMED; -.
DR PhylomeDB; Q8L500; -.
DR PRO; PR:Q8L500; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L500; baseline and differential.
DR Genevisible; Q8L500; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IPI:TAIR.
DR GO; GO:0007623; P:circadian rhythm; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0010017; P:red or far-red light signaling pathway; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR045279; ARR-like.
DR InterPro; IPR010402; CCT_domain.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR43874; PTHR43874; 1.
DR Pfam; PF06203; CCT; 1.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51017; CCT; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Biological rhythms; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..468
FT /note="Two-component response regulator-like APRR9"
FT /id="PRO_0000081441"
FT DOMAIN 38..156
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 417..459
FT /note="CCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00357"
FT REGION 168..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 302..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..327
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..367
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 381..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..416
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 468 AA; 52565 MW; 19D6C9571CA77A40 CRC64;
MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ IITALLRKCC
YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL LALVMEHEAC KNIPVIMMSS
QDSIKMVLKC MLRGAADYLI KPMRKNELKN LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE
DTDETCEDSR YHSDQGSGAQ AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR
PDSIYKDKSR DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK
AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR NQVLQSTVTN
QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE KPKEEESAKQ RWSRSQREAA
LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP RVKGQFVRTV NSDASTKS
