ISAM3_ARATH
ID ISAM3_ARATH Reviewed; 109 AA.
AC Q8L8C0; Q9SJN1;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Iron-sulfur assembly protein IscA-like 3, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At2g36260; ORFNames=F2H17.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the assembly of mitochondrial iron-sulfur
CC proteins. Probably involved in the binding of an intermediate of Fe/S
CC cluster assembly (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC Note=Binds 2 iron ions per dimer. The dimer may bind additional iron
CC ions. {ECO:0000250};
CC -!- SUBUNIT: Homodimer; may form tetramers and higher multimers.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the HesB/IscA family. {ECO:0000305}.
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DR EMBL; AC006921; AAD21439.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09224.1; -; Genomic_DNA.
DR EMBL; AY102552; AAM76757.1; -; mRNA.
DR EMBL; AY773862; AAV63891.1; -; mRNA.
DR PIR; F84778; F84778.
DR RefSeq; NP_181168.1; NM_129184.3.
DR AlphaFoldDB; Q8L8C0; -.
DR SMR; Q8L8C0; -.
DR STRING; 3702.AT2G36260.1; -.
DR PaxDb; Q8L8C0; -.
DR PRIDE; Q8L8C0; -.
DR ProteomicsDB; 247297; -.
DR EnsemblPlants; AT2G36260.1; AT2G36260.1; AT2G36260.
DR GeneID; 818198; -.
DR Gramene; AT2G36260.1; AT2G36260.1; AT2G36260.
DR KEGG; ath:AT2G36260; -.
DR Araport; AT2G36260; -.
DR TAIR; locus:2049440; AT2G36260.
DR eggNOG; KOG1120; Eukaryota.
DR HOGENOM; CLU_069054_4_2_1; -.
DR InParanoid; Q8L8C0; -.
DR OMA; GYQYGMA; -.
DR OrthoDB; 1578799at2759; -.
DR PhylomeDB; Q8L8C0; -.
DR PRO; PR:Q8L8C0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L8C0; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IBA:GO_Central.
DR Gene3D; 2.60.300.12; -; 1.
DR InterPro; IPR000361; FeS_biogenesis.
DR InterPro; IPR016092; FeS_cluster_insertion.
DR InterPro; IPR035903; HesB-like_dom_sf.
DR Pfam; PF01521; Fe-S_biosyn; 1.
DR SUPFAM; SSF89360; SSF89360; 1.
DR TIGRFAMs; TIGR00049; TIGR00049; 1.
PE 3: Inferred from homology;
KW Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..18
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 19..109
FT /note="Iron-sulfur assembly protein IscA-like 3,
FT mitochondrial"
FT /id="PRO_0000223686"
FT BINDING 37
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT BINDING 100
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT BINDING 102
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250|UniProtKB:P0AAC8"
SQ SEQUENCE 109 AA; 12162 MW; 238252575A598905 CRC64;
MRKQVLALSD TAAARIRQLL QHQQKPFLRL AVEAKGCNGL SYVLNYAQEK GKFDEVVEEK
GVKILVDPKA VMHVIGTEMD FVDDKLRSEF VFVNPNATKC GCGESFTTT
