ID   ISAMP_IXOSC             Reviewed;          69 AA.
AC   Q8MVA6;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Antimicrobial peptide ISAMP {ECO:0000303|PubMed:19852941};
DE   Flags: Precursor;
OS   Ixodes scapularis (Black-legged tick) (Deer tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX   NCBI_TaxID=6945;
RN   [1] {ECO:0000312|EMBL:AAM93656.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=Rhode Island {ECO:0000312|EMBL:AAM93656.1};
RC   TISSUE=Salivary gland {ECO:0000312|EMBL:AAM93656.1};
RX   PubMed=12177149; DOI=10.1242/jeb.205.18.2843;
RA   Valenzuela J.G., Francischetti I.M., Pham V.M., Garfield M.K., Mather T.N.,
RA   Ribeiro J.M.;
RT   "Exploring the sialome of the tick Ixodes scapularis.";
RL   J. Exp. Biol. 205:2843-2864(2002).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 24-42, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND MASS SPECTROMETRY.
RC   STRAIN=Rhode Island {ECO:0000269|PubMed:19852941};
RC   TISSUE=Saliva {ECO:0000269|PubMed:19852941};
RX   PubMed=19852941; DOI=10.1016/j.bbrc.2009.09.127;
RA   Pichu S., Ribeiro J.M., Mather T.N.;
RT   "Purification and characterization of a novel salivary antimicrobial
RT   peptide from the tick, Ixodes scapularis.";
RL   Biochem. Biophys. Res. Commun. 390:511-515(2009).
CC   -!- FUNCTION: Has antimicrobial activity against B.cereus (MIC=5.8 ug/ml),
CC       B.subtilis (MIC=12.3 ug/ml), S.aureus (MIC=10.4 ug/ml), E.coli Edl 933
CC       (MIC=3.2 ug/ml) and E.coli MG/655 (MIC=4.2 ug/ml). Non-hemolytic.
CC       {ECO:0000269|PubMed:19852941}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12177149,
CC       ECO:0000269|PubMed:19852941}.
CC   -!- TISSUE SPECIFICITY: Expressed in the fat body, hemocytes and salivary
CC       glands of partially-fed female ticks. Not expressed in the midgut.
CC       {ECO:0000269|PubMed:19852941}.
CC   -!- MASS SPECTROMETRY: Mass=5300.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19852941};
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DR   EMBL; AF483734; AAM93656.1; -; mRNA.
DR   AlphaFoldDB; Q8MVA6; -.
DR   VEuPathDB; VectorBase:ISCI008031; -.
DR   VEuPathDB; VectorBase:ISCW008031; -.
DR   Proteomes; UP000001555; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0042742; P:defense response to bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:19852941"
FT   PEPTIDE         24..69
FT                   /note="Antimicrobial peptide ISAMP"
FT                   /evidence="ECO:0000269|PubMed:19852941"
FT                   /id="PRO_0000391699"
SQ   SEQUENCE   69 AA;  7817 MW;  EBB93FDBFA754E24 CRC64;
     MRAVAIFIVT LLVLECVYFV MSEPDPGQPW QVKAGRPPCY SIPCRKHDEC RVGSCSRCNN
     GLWGDRTCR