ISC1_PHYSP
ID ISC1_PHYSP Reviewed; 210 AA.
AC G4YEI5;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Secreted isochorismatase effector Isc1 {ECO:0000303|PubMed:25156390};
DE EC=3.3.2.1 {ECO:0000269|PubMed:25156390};
GN Name=Isc1 {ECO:0000303|PubMed:25156390}; ORFNames=PHYSODRAFT_467899;
OS Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS (Phytophthora megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1094619;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6497;
RX PubMed=16946064; DOI=10.1126/science.1128796;
RA Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H.Y., Aerts A.,
RA Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA Damasceno C.M.B., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.-K.,
RA McDonald W.H., Medina M., Meijer H.J.G., Nordberg E.K., Maclean D.J.,
RA Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA Rose J.K.C., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA Smith B.M., Sobral B.W.S., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT of pathogenesis.";
RL Science 313:1261-1266(2006).
RN [2]
RP INDUCTION, DISRUPTION PHENOTYPE, FUNCTION, ACTIVE SITE, MUTAGENESIS OF
RP ASP-25; LYS-90 AND CYS-124, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=25156390; DOI=10.1038/ncomms5686;
RA Liu T., Song T., Zhang X., Yuan H., Su L., Li W., Xu J., Liu S., Chen L.,
RA Chen T., Zhang M., Gu L., Zhang B., Dou D.;
RT "Unconventionally secreted effectors of two filamentous pathogens target
RT plant salicylate biosynthesis.";
RL Nat. Commun. 5:4686-4686(2014).
CC -!- FUNCTION: Secreted isochorismatase required for full virulence of
CC P.sojae (PubMed:25156390). Suppresses salicylate-mediated innate
CC immunity of the host by disrupting the plant salicylate metabolism
CC pathway via hydrolysis of its isochorismate precursor
CC (PubMed:25156390). {ECO:0000269|PubMed:25156390}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC Evidence={ECO:0000269|PubMed:25156390};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25156390}. Host
CC cytoplasm {ECO:0000269|PubMed:25156390}. Host nucleus
CC {ECO:0000269|PubMed:25156390}. Note=Lacks a signal peptide and uses an
CC unconventional secretion pathway for delivering effectors which plays
CC an important role in host-pathogen interactions.
CC {ECO:0000269|PubMed:25156390}.
CC -!- INDUCTION: Expression is up-regulated during the infection stages.
CC {ECO:0000269|PubMed:25156390}.
CC -!- DISRUPTION PHENOTYPE: Leads to a significant reduction in virulence on
CC soybean seedling and increases the concentrations of both free
CC salicylate and its glucoside (SAG) by about 3-fold in soybean seedling.
CC {ECO:0000269|PubMed:25156390}.
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR EMBL; JH159151; EGZ27262.1; -; Genomic_DNA.
DR RefSeq; XP_009514537.1; XM_009516242.1.
DR AlphaFoldDB; G4YEI5; -.
DR SMR; G4YEI5; -.
DR STRING; 1094619.G4YEI5; -.
DR EnsemblProtists; EGZ27262; EGZ27262; PHYSODRAFT_467899.
DR GeneID; 20653615; -.
DR KEGG; psoj:PHYSODRAFT_467899; -.
DR InParanoid; G4YEI5; -.
DR OMA; LIVTEHY; -.
DR Proteomes; UP000002640; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Hydrolase; Reference proteome; Secreted;
KW Virulence.
FT CHAIN 1..210
FT /note="Secreted isochorismatase effector Isc1"
FT /id="PRO_0000448092"
FT ACT_SITE 25
FT /evidence="ECO:0000269|PubMed:25156390"
FT ACT_SITE 90
FT /evidence="ECO:0000269|PubMed:25156390"
FT ACT_SITE 124
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 25
FT /note="D->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-90 and A-
FT 124."
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 90
FT /note="K->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-25 and A-
FT 124."
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 124
FT /note="C->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-25 and A-90."
FT /evidence="ECO:0000269|PubMed:25156390"
SQ SEQUENCE 210 AA; 23015 MW; 4E8CD2E5937318EA CRC64;
MAALSSATKR LGRLIPHSSV LFVCDVQEVF RGLTFQLPTV IHGTNTMVSA AKLLNVPVVV
TTQYGSRLGS TVSEISKNLE DAPDVKVFDK MKFSMLVPEV ERHLTTNMPQ RKSVLLCGIE
THVCVLQTCL DLLDKGYDVH VVSDAVSSST SYNRSMALER IRQSGAYITS VESAIFQLAN
DAGNPEFKSI SKLIKEHLKV QNGFDTGARI
